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公开(公告)号：US20220389434A1
公开(公告)日：2022-12-08
申请号：US17844371
申请日：2022-06-20
Applicant: Promega Corporation
Inventor： Lance P. Encell , Mary Hall , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kristopher Zimmerman
IPC: C12N15/52
Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO: 1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
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公开(公告)号：US20190185908A1
公开(公告)日：2019-06-20
申请号：US16294464
申请日：2019-03-06
Applicant: Promega Corporation
Inventor： Lance P. Encell , Mary Hall , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kristopher Zimmerman
CPC classification number: C12Q1/66 , C12N9/0004
Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
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3.发明申请公开(公告)号：US20170137793A1
公开(公告)日：2017-05-18
申请号：US15414291
申请日：2017-01-24
Applicant: Promega Corporation
Inventor： Aldis Darzins , Lance P. Encell , Rachel Friedman Ohana , Paul Otto , Michael R. Slater , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kate Qin Zhao , Kris Zimmerman
CPC classification number: C12N9/14 , C12N9/96 , C12N11/00 , C12Q1/34 , C12Y308/01 , G01N33/581
Abstract: The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
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公开(公告)号：US20170115283A1
公开(公告)日：2017-04-27
申请号：US15402879
申请日：2017-01-10
Applicant: Promega Corporation
Inventor： Carolyn W. Hitko , Robin Hurst , Thomas Kirkland , Dieter Klaubert , Poncho Meisenheimer , Nidhi Nath , Rachel Friedman Ohana , Paul Otto , Marjeta Urh , Harry Tetsuo Uyeda , Keith Wood , Ji Zhu
IPC: G01N33/543
CPC classification number: G01N33/54333 , G01N33/54306 , G01N33/54326
Abstract: The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture fusions), surfaces (e.g., displaying, capture ligands, capture proteins, or capture fusions), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.
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公开(公告)号：US11365436B2
公开(公告)日：2022-06-21
申请号：US17075415
申请日：2020-10-20
Applicant: Promega Corporation
Inventor： Lance P. Encell , Mary Hall , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kristopher Zimmerman
Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
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Patent Agency Ranking
6.发明授权Polynucleotides encoding mutant hydrolase proteins with enhanced kinetics and functional expression 有权编码突变体水解酶蛋白的多核苷酸具有增强的动力学和功能表达公开(公告)号：US09593316B2
公开(公告)日：2017-03-14
申请号：US14285327
申请日：2014-05-22
Applicant: Promega Corporation
Inventor： Aldis Darzins , Lance P. Encell , Rachel Friedman Ohana , Paul Otto , Michael R. Slater , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kate Qin Zhao , Kris Zimmerman
CPC classification number: C12N9/14 , C12N9/96 , C12N11/00 , C12Q1/34 , C12Y308/01 , G01N33/581
Abstract: The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
Abstract translation: 本发明提供具有增强的动力学和功能表达的突变水解酶蛋白，以及编码突变蛋白的多核苷酸以及使用多核苷酸和突变蛋白的方法。
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公开(公告)号：US09339561B2
公开(公告)日：2016-05-17
申请号：US14269669
申请日：2014-05-05
Applicant: Promega Corporation
Inventor： Brock Binkowski , Braeden Butler , Lance P. Encell , Frank Fan , Brad Hook , Paul Otto , Gediminas Vidugiris , Susan Wigdal , Kristopher Zimmerman
IPC: A61K49/00 , C12N9/02 , C12Q1/37 , C12Q1/66 , A01K67/027
CPC classification number: A61K49/0045 , A01K67/0275 , A61K49/0013 , C12N9/0069 , C12Q1/37 , C12Q1/66 , C12Y113/12007
Abstract: A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.
Abstract translation: 编码包含修饰的环状置换的热稳定性荧光素酶的生物传感器多肽的多核苷酸和将热稳定性荧光素酶的C末端部分连接到热稳定性萤光素酶的N末端部分的接头。 相对于亲本循环置换的热稳定性荧光素酶，修饰的环状置换的热稳定性荧光素酶被修饰。 接头包含能够与细胞中的靶分子相互作用的传感器区域。 经修饰的环状置换的热稳定荧光素酶在目标分子存在下相对于亲本循环置换的热稳定性荧光素酶在生物传感器与靶分子相互作用后具有增强的响应。 或者，修饰的环状置换的热稳定荧光素酶在生物传感器与目标分子相互作用后相对于在不存在目标分子的情况下相对于经修饰的环状置换的热稳定性荧光素酶具有增强的响应。
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公开(公告)号：US09290794B2
公开(公告)日：2016-03-22
申请号：US13674655
申请日：2012-11-12
Applicant: PROMEGA CORPORATION
Inventor： Brock Binkowski , Braeden Butler , Lance P. Encell , Frank Fan , Brad Hook , Paul Otto , Kimberly Risma , Gediminas Vidugiris , Susan Wigdal , Kristopher Zimmerman
CPC classification number: C12Q1/66 , C12N9/0069 , C12Q1/37
Abstract: A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.
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公开(公告)号：US20180051318A1
公开(公告)日：2018-02-22
申请号：US15714210
申请日：2017-09-25
Applicant: Promega Corporation
Inventor： Lance P. Encell , Mary Hall , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kristopher Zimmerman
CPC classification number: C12Q1/66 , C12N9/0004
Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
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公开(公告)号：US09777311B2
公开(公告)日：2017-10-03
申请号：US14053252
申请日：2013-10-14
Applicant: PROMEGA CORPORATION
Inventor： Lance P. Encell , Mary Hall , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kristopher Zimmerman
CPC classification number: C12Q1/66 , C12N9/0004
Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
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