公开(公告)号：US06965113B2

公开(公告)日：2005-11-15

申请号：US09779461

申请日：2001-02-09

申请人： Peet Kask ,  Stefan Jäger ,  Kaupo Palo ,  Ülo Mets ,  Karsten Gall

发明人： Peet Kask ,  Stefan Jäger ,  Kaupo Palo ,  Ülo Mets ,  Karsten Gall

IPC分类号： G01J3/28 ,  G01J3/44 ,  G01J3/457 ,  G01N21/64

CPC分类号： G01N21/64 ,  G01J3/4406 ,  G01J3/457 ,  G01J2003/2866 ,  G01N21/6408 ,  G01N21/6458 ,  G01N2021/6421 ,  G01N2021/6463

摘要： A method for distinguishing samples having flourescent particles includes (a) monitoring intensity fluctuations of fluorescence emitted by the particles in at least one measurement volume by detecting sequences of photon counts by at least one photon detector, (b) determining, from the sequences of photon counts, intermediate statistical data involving at least two probability functions of the number of photon counts detected in different sets of counting time intervals, and (c) determining from the intermediate statistical data a distribution of particles as a function of at least two arguments, wherein one argument is a specific brightness of the particles, or a measure thereof, and another argument is a diffusion coefficient of the particles, or a measure thereof.

摘要翻译： 用于区分具有荧光颗粒的样品的方法包括（a）通过至少一个光子检测器检测光子计数序列来监测由至少一个测量体积中的颗粒发射的荧光的强度波动，（b）从光子序列 计数涉及在不同的计数时间间隔集中检测的光子计数的至少两个概率函数的中间统计数据，以及（c）从中间统计数据确定作为至少两个参数的函数的粒子分布，其中 一个参数是颗粒的特定亮度或其度量，另一个参数是颗粒的扩散系数或其度量。