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    Method for detecting an analyte in a sample
    2.
    发明授权
    Method for detecting an analyte in a sample 有权
    检测样品中分析物的方法

    公开(公告)号：US08580577B2

    公开(公告)日：2013-11-12

    申请号：US12662896

    申请日：2010-05-10

    申请人： Silke Hinnah ,  Dagmar Lambrü ,  Sonja Dröge ,  Stefan Jäger ,  Karsten Gall ,  Werner Stürmer ,  Michaela Schäfer

    发明人： Silke Hinnah ,  Dagmar Lambrü ,  Sonja Dröge ,  Stefan Jäger ,  Karsten Gall ,  Werner Stürmer ,  Michaela Schäfer

    IPC分类号： G01N33/566 ,  G01N33/53 ,  G01N33/543 ,  G01N33/554 ,  G01N21/00 ,  G01N21/76 ,  C12Q1/68

    CPC分类号： C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6837 ,  G01N33/54306 ,  C12Q2565/101 ,  C12Q2545/114 ,  C12Q2539/101 ,  C12Q2537/125

    摘要： The present invention relates to a method for detecting an analyte in a sample comprising the steps of providing detection probes being labeled with a first reporter, which detection probes are capable of binding to the analyte, providing a solid support, providing capture probes being bound or capable of binding to the solid support, which capture probes are capable of binding to the analyte, thus concentrating the analyte on the solid support, contacting the sample with the detection probes, the solid support and the capture probes, and detecting the detection probes, wherein the detection of detection probes is conducted in the presence of quenching probes binding to surplus detection probes not being bound to the analyte and thereby quenching at least partially an emission of the first reporter of said surplus detection probes and/or the solid support is labeled with a second reporter different from the first reporter, imaging the sample at an emission wavelength of the second reporter, generating a mask obtained from imaging the sample at the emission wavelength of the second reporter and applying this mask to an image of the sample used for detecting the detection probes.

    摘要翻译： 本发明涉及一种用于检测样品中分析物的方法，包括以下步骤：提供用第一报道分子标记的检测探针，该检测探针能够结合分析物，提供固体支持物，提供结合的捕获探针或 能够结合固体支持物，其捕获探针能够结合分析物，从而将分析物浓缩在固体支持物上，使样品与检测探针，固体支持物和捕获探针接触，并检测检测探针， 其中所述检测探针的检测是在存在猝灭探针的情况下进行的，所述猝灭探针结合不结合分析物的剩余检测探针，从而至少部分地猝灭所述剩余检测探针和/或固体支持物的第一报告物的发射被标记 与第二个记者不同的第二个记者，以第二个回购的发射波长成像样品 产生从第二报道器的发射波长处成像样品获得的掩模，并将该掩模应用于用于检测检测探针的样品的图像。

    Method for detecting an analyte in a sample
    3.
    发明申请
    Method for detecting an analyte in a sample 有权
    检测样品中分析物的方法

    公开(公告)号：US20110105348A1

    公开(公告)日：2011-05-05

    申请号：US12662896

    申请日：2010-05-10

    申请人： Silke Hinnah ,  Dagmar Lambrü ,  Sonja Dröge ,  Stefan Jäger ,  Karsten Gall ,  Werner Stürmer ,  Michaela Schäfer

    发明人： Silke Hinnah ,  Dagmar Lambrü ,  Sonja Dröge ,  Stefan Jäger ,  Karsten Gall ,  Werner Stürmer ,  Michaela Schäfer

    IPC分类号： C40B30/04 ,  G01N33/53

    CPC分类号： C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6837 ,  G01N33/54306 ,  C12Q2565/101 ,  C12Q2545/114 ,  C12Q2539/101 ,  C12Q2537/125

    摘要： The present invention relates to a method for detecting an analyte in a sample comprising the steps of providing detection probes being labeled with a first reporter, which detection probes are capable of binding to the analyte, providing a solid support, providing capture probes being bound or capable of binding to the solid support, which capture probes are capable of binding to the analyte, thus concentrating the analyte on the solid support, contacting the sample with the detection probes, the solid support and the capture probes, and detecting the detection probes, wherein the detection of detection probes is conducted in the presence of quenching probes binding to surplus detection probes not being bound to the analyte and thereby quenching at least partially an emission of the first reporter of said surplus detection probes and/or the solid support is labeled with a second reporter different from the first reporter, imaging the sample at an emission wavelength of the second reporter, generating a mask obtained from imaging the sample at the emission wavelength of the second reporter and applying this mask to an image of the sample used for detecting the detection probes.

    摘要翻译： 本发明涉及一种用于检测样品中分析物的方法，包括以下步骤：提供用第一报道分子标记的检测探针，该检测探针能够结合分析物，提供固体支持物，提供结合的捕获探针或 能够结合固体支持物，其捕获探针能够结合分析物，从而将分析物浓缩在固体支持物上，使样品与检测探针，固体支持物和捕获探针接触，并检测检测探针， 其中所述检测探针的检测是在存在猝灭探针的情况下进行的，所述猝灭探针结合不结合分析物的剩余检测探针，从而至少部分地猝灭所述剩余检测探针和/或固体支持物的第一报告物的发射被标记 与第二个记者不同的第二个记者，以第二个回购的发射波长成像样品 产生从第二报道器的发射波长处成像样品获得的掩模，并将该掩模应用于用于检测检测探针的样品的图像。

    Gas generator
    5.
    发明授权
    Gas generator 有权
    气体发生器

    公开(公告)号：US07669893B2

    公开(公告)日：2010-03-02

    申请号：US12216923

    申请日：2008-07-11

    申请人： Stefan Jäger ,  Thomas Dirnberger ,  Christophe Dervyn

    发明人： Stefan Jäger ,  Thomas Dirnberger ,  Christophe Dervyn

    IPC分类号： B60R21/264

    CPC分类号： B60R21/2644 ,  B60R2021/26029 ,  Y10T29/49925

    摘要： A method of producing a gas generator includes arranging an igniter in an inner chamber surrounded by a housing of the gas generator. The igniter is supported in a first direction on a part of the gas generator and in the first direction opposite a mounting orifice of the housing. The method includes arranging a sealing element in the mounting orifice to seal the mounting orifice. The sealing element presses over a bearing area deformable in the first direction with a force acting in the first direction against a first bearing area of the igniter and forms a gas-tight joint between the sealing element and the igniter. A side of the sealing element facing the part of the gas generator in the first direction abuts against a side of the part of the gas generator to limit deformation of the bearing area of the sealing element in the first direction.

    摘要翻译： 一种生产气体发生器的方法包括将点火器布置在由气体发生器的壳体包围的内室中。 点火器在气体发生器的一部分上沿着第一方向并且在与壳体的安装孔相对的第一方向上被支撑。 该方法包括在安装孔中布置密封元件以密封安装孔。 密封元件压靠在可沿着第一方向变形的支承区域上，其中第一方向上的作用力抵靠点火器的第一支承区域，并在密封元件和点火器之间形成气密接头。 在第一方向上面对气体发生器的一部分的密封元件的一侧与气体发生器的一部分相邻，以限制密封元件在第一方向上的轴承区域的变形。

    Fluorescence intensity multiple distributions analysis: concurrent determination of diffusion times and molecular brightness
    7.
    发明授权
    Fluorescence intensity multiple distributions analysis: concurrent determination of diffusion times and molecular brightness 有权
    荧光强度多重分布分析：同时测定扩散时间和分子亮度

    公开(公告)号：US06965113B2

    公开(公告)日：2005-11-15

    申请号：US09779461

    申请日：2001-02-09

    申请人： Peet Kask ,  Stefan Jäger ,  Kaupo Palo ,  Ülo Mets ,  Karsten Gall

    发明人： Peet Kask ,  Stefan Jäger ,  Kaupo Palo ,  Ülo Mets ,  Karsten Gall

    IPC分类号： G01J3/28 ,  G01J3/44 ,  G01J3/457 ,  G01N21/64

    CPC分类号： G01N21/64 ,  G01J3/4406 ,  G01J3/457 ,  G01J2003/2866 ,  G01N21/6408 ,  G01N21/6458 ,  G01N2021/6421 ,  G01N2021/6463

    摘要： A method for distinguishing samples having flourescent particles includes (a) monitoring intensity fluctuations of fluorescence emitted by the particles in at least one measurement volume by detecting sequences of photon counts by at least one photon detector, (b) determining, from the sequences of photon counts, intermediate statistical data involving at least two probability functions of the number of photon counts detected in different sets of counting time intervals, and (c) determining from the intermediate statistical data a distribution of particles as a function of at least two arguments, wherein one argument is a specific brightness of the particles, or a measure thereof, and another argument is a diffusion coefficient of the particles, or a measure thereof.

    摘要翻译： 用于区分具有荧光颗粒的样品的方法包括（a）通过至少一个光子检测器检测光子计数序列来监测由至少一个测量体积中的颗粒发射的荧光的强度波动，（b）从光子序列 计数涉及在不同的计数时间间隔集中检测的光子计数的至少两个概率函数的中间统计数据，以及（c）从中间统计数据确定作为至少两个参数的函数的粒子分布，其中 一个参数是颗粒的特定亮度或其度量，另一个参数是颗粒的扩散系数或其度量。