公开(公告)号：US10197551B2

公开(公告)日：2019-02-05

申请号：US14679114

申请日：2015-04-06

申请人： ASTA, Co. Ltd.

发明人： Eung Joon Jo

IPC分类号： H01J49/00 ,  G01N33/483 ,  G01N33/49 ,  H01J49/16 ,  H01J49/40

摘要： A disease diagnostic system where a sample preparation unit and/or a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS) data generation unit may be integrated in one system or a set of a system to improve the user-friendliness of the system. The system may include a sample preparation unit with processing modules and/or a handler to move samples in an autonomous manner to enhance reproducibility of measurement data and/or user-friendliness. A different set of processing modules may be selected for a particular disease type (e.g. such as cancer) to be diagnosed. The system may be used to identify biomaterials such as bacteria, virus, and fungi from body fluids like blood, urine, and saliva and other cells.

公开(公告)号：US20210118658A1

公开(公告)日：2021-04-22

申请号：US17134571

申请日：2020-12-28

申请人： ASTA, CO. LTD.

发明人： Eung Joon JO

IPC分类号： H01J49/00 ,  G01N33/483 ,  H01J49/40 ,  H01J49/16 ,  G01N33/49

摘要： A disease diagnostic system where a sample preparation unit and/or a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS) data generation unit may be integrated in one system or a set of a system to improve the user-friendliness of the system. The system may include a sample preparation unit with processing modules and/or a handler to move samples in an autonomous manner to enhance reproducibility of measurement data and/or user-friendliness. A different set of processing modules may be selected for a particular disease type (e.g. such as cancer) to be diagnosed. The system may be used to identify biomaterials such as bacteria, virus, and fungi from body fluids like blood, urine, and saliva and other cells.

公开(公告)号：US10481086B2

公开(公告)日：2019-11-19

申请号：US16088432

申请日：2016-10-28

申请人： ASTA CO., LTD.

发明人： Jooyeon Oh ,  Yang Sun Kim

IPC分类号： G01N21/359 ,  G01N27/62 ,  G01N21/64 ,  H01J49/26 ,  H01J49/16

摘要： A mass spectrometer using near infrared (NIR) fluorescence can comprise: a plate formed such that a sample, which includes a matrix for ionization and a fluorescent material having an excitation wavelength of an NIR wavelength band, is loaded thereon; a fluorescence detection unit formed so as to acquire a fluorescence image by using the fluorescent material from the sample on the plate; a light emission unit formed so as to emit a laser beam for ionization at the sample on the plate; and an ion detection unit formed so as to detect, by using the laser beam, ions generated from the sample. By using the NIR fluorescence, fluorescence interference due to a matrix used for MALDI imaging can be excluded even after coating the matrix, and since fluorescence interference due to auto-fluorescence inside tissue is low, fluorescence can be measured even for a thick sample.

公开(公告)号：US20160148793A1

公开(公告)日：2016-05-26

申请号：US14759160

申请日：2014-01-03

申请人： ASTA CO., LTD.

发明人： Myung Soo KIM ,  Yong Jin BAE

IPC分类号： H01J49/00 ,  H01J49/16

CPC分类号： H01J49/0036 ,  H01J49/0009 ,  H01J49/0027 ,  H01J49/164

摘要： A method for obtaining a mass spectrum of reproducible ions generated at a constant temperature by measuring a total ion count, and the use of a matrix for quantitative analysis using MALDI mass spectrometry are disclosed. More particularly, a method for measuring a mass spectrum of ions generated at a constant temperature may include selecting only mass spectra having the same total ion count, among multiple mass spectra obtained from ions formed by applying energy to a specimen having a sample mixed therein.

摘要翻译： 公开了通过测量总离子计数获得在恒定温度下产生的可重现离子的质谱的方法，以及使用MALDI质谱法进行定量分析的基质。 更具体地，用于测量在恒定温度下产生的离子的质谱的方法可以包括在从通过向其中混合有样品的样品施加能量而形成的离子获得的多个质谱中仅选择具有相同总离子计数的质谱。

公开(公告)号：US09349578B2

公开(公告)日：2016-05-24

申请号：US14759168

申请日：2014-01-03

申请人： ASTA CO., LTD.

发明人： Myung Soo Kim ,  Yong Jin Bae

IPC分类号： H01J49/00 ,  H01J49/16

CPC分类号： H01J49/0031 ,  H01J49/0036 ,  H01J49/164

摘要： A quantitative analysis method using MALDI mass spectrometry wherein laser pulse energy is adjusted is disclosed. More particularly, a method for measuring the equilibrium constant of a proton exchange reaction between a matrix and a sample at a constant temperature, by dividing an ion signal ratio by a value (concentration ratio) obtained by dividing the concentration of the sample by the concentration of the matrix, may include (i) obtaining MALDI mass spectra having constant TICs by adjusting the intensity of energy applied to a specimen having a predetermined amount of a matrix and a predetermined amount of a sample mixed therein and (ii) measuring the MALDI mass spectra obtained in step (i) for a value (ion signal ratio) obtained by dividing sample ion signal strength by matrix ion signal strength.

摘要翻译： 公开了使用MALDI质谱法的定量分析方法，其中调整激光脉冲能量。 更具体地说，一种用于通过将离子信号比除以样品浓度除以浓度得到的值（浓度比）来测量恒定温度下的基体和样品之间的质子交换反应的平衡常数的方法 可以包括（i）通过调整施加到具有预定量的基质和预定量的样品的样品的能量的强度来获得具有恒定TIC的MALDI质谱，和（ii）测量MALDI质量 通过将样本离子信号强度除以基质离子信号强度获得的值（离子信号比），在步骤（i）中获得的光谱。

公开(公告)号：US11348776B2

公开(公告)日：2022-05-31

申请号：US17134571

申请日：2020-12-28

申请人： ASTA, CO. LTD.

发明人： Eung Joon Jo

IPC分类号： H01J49/40 ,  H01J49/00 ,  H01J49/16 ,  G01N33/49 ,  G01N33/483

摘要： A disease diagnostic system where a sample preparation unit and/or a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS) data generation unit may be integrated in one system or a set of a system to improve the user-friendliness of the system. The system may include a sample preparation unit with processing modules and/or a handler to move samples in an autonomous manner to enhance reproducibility of measurement data and/or user-friendliness. A different set of processing modules may be selected for a particular disease type (e.g. such as cancer) to be diagnosed. The system may be used to identify biomaterials such as bacteria, virus, and fungi from body fluids like blood, urine, and saliva and other cells.

公开(公告)号：US20190139747A1

公开(公告)日：2019-05-09

申请号：US16232456

申请日：2018-12-26

申请人： ASTA, CO. LTD.

发明人： Eung Joon JO

IPC分类号： H01J49/00 ,  H01J49/16 ,  H01J49/40 ,  G01N33/483 ,  G01N33/49

摘要： A disease diagnostic system where a sample preparation unit and/or a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS) data generation unit may be integrated in one system or a set of a system to improve the user-friendliness of the system. The system may include a sample preparation unit with processing modules and/or a handler to move samples in an autonomous manner to enhance reproducibility of measurement data and/or user-friendliness. A different set of processing modules may be selected for a particular disease type (e.g. such as cancer) to be diagnosed. The system may be used to identify biomaterials such as bacteria, virus, and fungi from body fluids like blood, urine, and saliva and other cells.

公开(公告)号：US20220277945A1

公开(公告)日：2022-09-01

申请号：US17744679

申请日：2022-05-15

申请人： ASTA, CO. LTD.

发明人： Eung Joon JO

IPC分类号： H01J49/00 ,  H01J49/16 ,  G01N33/49 ,  H01J49/40 ,  G01N33/483

摘要： An analysis system and/or method including a mass spectrometer. The mass spectrometer may be configured to conduct a measurement on a sample using at least one laser-shot and generate a distribution of measured data for the at least one laser-shot on the sample. The measurement on the sample is a manufacturing technique. The manufacturing technique relates to component costs.

公开(公告)号：US20190113448A1

公开(公告)日：2019-04-18

申请号：US16088432

申请日：2016-10-28

申请人： ASTA CO., LTD.

发明人： Jooyeon Oh ,  Yang Sun Kim

IPC分类号： G01N21/359 ,  G01N21/64 ,  G01N27/62 ,  H01J49/26

摘要： A mass spectrometer using near infrared (NIR) fluorescence can comprise: a plate formed such that a sample, which includes a matrix for ionization and a fluorescent material having an excitation wavelength of an NIR wavelength band, is loaded thereon; a fluorescence detection unit formed so as to acquire a fluorescence image by using the fluorescent material from the sample on the plate; a light emission unit formed so as to emit a laser beam for ionization at the sample on the plate; and an ion detection unit formed so as to detect, by using the laser beam, ions generated from the sample. By using the NIR fluorescence, fluorescence interference due to a matrix used for MALDI imaging can be excluded even after coating the matrix, and since fluorescence interference due to auto-fluorescence inside tissue is low, fluorescence can be measured even for a thick sample.

公开(公告)号：US09646810B2

公开(公告)日：2017-05-09

申请号：US14415302

申请日：2013-07-17

申请人： SNU R&DB FOUNDATION ,  ASTA CO. LTD.

发明人： Taeghwan Hyeon ,  Myung Soo Kim ,  Yong Jin Bae ,  Kyung Man Park ,  Sung Hee Ahn

IPC分类号： H01J49/00 ,  H01J49/16

CPC分类号： H01J49/0009 ,  H01J49/0027 ,  H01J49/164

摘要： Methods are described for improving reproducibility of mass spectrum and quantitative analysis method using the same. More particularly, methods for improving reproducibility of a mass spectrum of a chemical compound, wherein temperatures of an ion generation reaction are controlled to be the same with each other, or wherein spectra of which temperature of ion generation reaction are the same with each other are selected from mass spectra of a chemical compound are described. In addition, methods for measuring an equilibrium constant of a proton transfer reaction between a matrix and an analyte at a certain temperature, for obtaining a calibration curve for quantitative analysis, and for quantitative analysis of an analyte by using mass spectra area described.