公开(公告)号：US06600028B1

公开(公告)日：2003-07-29

申请号：US09402048

申请日：2000-02-01

Applicant: Daniel Brown ,  David Loakes ,  David Williams ,  Fergal Hill ,  Shiv Kumar ,  Satyam Nampalli ,  Mark McDougall ,  Alan Hamilton ,  Clifford Smith ,  Adrian Christopher Simmonds ,  William Jonathan Cummins ,  Patrick Finn

Inventor： Daniel Brown ,  David Loakes ,  David Williams ,  Fergal Hill ,  Shiv Kumar ,  Satyam Nampalli ,  Mark McDougall ,  Alan Hamilton ,  Clifford Smith ,  Adrian Christopher Simmonds ,  William Jonathan Cummins ,  Patrick Finn

IPC: C07H2100

CPC classification number: C07D498/16 ,  C07H19/04 ,  C07H21/00

Abstract: Nucleoside analogues have structure (2) wherein Q is H or a sugar moiety or sugar analogue or a modified sugar or a nucleic backbone or backbone analogue, W is an alkylene or alkenylene chain of 0-5 carbon atoms, any of which may carry a substituent R8, X is O or N or NR12 or CR10, X′ is O or S or N, provided that when X′ is O or S, then X is C, Y is CH or N, R6 is NH2 or SMe or SO2Me or NHNH2, each of R7 and R8 is independently H or F or alkyl or alkenyl or aryl or acyl or a reporter moiety, R12 is independently H or alkyl or alkenyl or aryl or acyl or a reporter moiety, and R10 is H or ═O or F or alkyl or aryl or a reporter moiety.

Abstract translation: 核苷类似物具有结构（2），其中Q是H或糖部分或糖类似物或修饰的糖或核心或骨架类似物，W是0-5个碳原子的亚烷基或亚烯基链，其中任何一个可携带 取代基R8，X为O或N或NR12或CR10，X'为O或S或N，条件是当X'为O或S时，则X为C，Y为CH或N，R6为NH2或SMe或SO2Me 或NHNH 2，R 7和R 8各自独立地为H或F，或烷基或链烯基或芳基或酰基或报道部分，R 12独立地为H或烷基或链烯基或芳基或酰基或报道部分，R 10为H或= O 或F或烷基或芳基或报道部分。

公开(公告)号：US06444682B1

公开(公告)日：2002-09-03

申请号：US09463501

申请日：2000-04-18

Applicant: Adrian Christopher Simmonds ,  Alan Hamilton ,  Clifford Smith ,  David Loakes ,  Daniel Brown ,  Fergal Hill ,  Shiv Kumar ,  Satyam Nampalli ,  Mark McDougall

Inventor： Adrian Christopher Simmonds ,  Alan Hamilton ,  Clifford Smith ,  David Loakes ,  Daniel Brown ,  Fergal Hill ,  Shiv Kumar ,  Satyam Nampalli ,  Mark McDougall

IPC: A61K3170

CPC classification number: G01N33/5308 ,  C07H21/00

Abstract: Nucleotide or base analogues having structure (3) or (4) wherein X═O or NH or S and each R6 is independently H or alkyl or alkenyl or alkoxy or aryl or a reporter moiety.

Abstract translation: 具有结构（3）或（4）的核苷酸或碱基类似物，其中X = O或NH或S，每个R6独立地为H或烷基或链烯基，烷氧基或芳基或报道部分。

公开(公告)号：US07511155B2

公开(公告)日：2009-03-31

申请号：US10519433

申请日：2002-07-08

Applicant: Karen Williams ,  Timothy Stone ,  Adrian Christopher Simmonds ,  Alison Claire Sweet ,  Susan Janet Fowler

Inventor： Karen Williams ,  Timothy Stone ,  Adrian Christopher Simmonds ,  Alison Claire Sweet ,  Susan Janet Fowler

IPC: C09B23/02 ,  C09B67/22 ,  G01N33/68

CPC classification number: C07K1/13 ,  C09B23/02 ,  C09B67/0034 ,  G01N33/6815

Abstract: A matched set of fluorescent dyes is provided, wherein each dye of the set is capable of covalent attachment to a protein and wherein each of the dyes has a molecular structure and a charge that is matched one with the other, such that relative electrophoretic mobility of a protein labeled with one dye of the set is the same as the electrophoretic mobility of the protein labeled with a different dye of the set. The matched set comprises at least two different fluorescent dyes of formula: wherein n is 1, 2, or 3; Z1 and Z2 independently represent the carbon atoms necessary to complete a phenyl or naphthyl ring system; one of groups R1 and R2 is a target bonding group; remaining group R1 or R2 is selected from —(CH2)4—W or —(CH2)r—H; group R3 is hydrogen, except when either R1 or R2 is —(CH2)r—H, in which case R3 is W; and W is selected from sulphonic acid and sulphonate. The invention also provides a method for saturation labeling of a protein with a fluorescent dye so as to label all available target amino acid, suitably cysteine, residues in the protein, thereby giving a single population of labeled protein molecules.

Abstract translation: 提供了一组匹配的荧光染料，其中该组的每种染料能够与蛋白质共价连接，并且其中每种染料具有彼此匹配的分子结构和电荷，使得相对电泳迁移率 用该组中的一种染料标记的蛋白质与用该组不同染料标记的蛋白质的电泳迁移率相同。 匹配组包含至少两种下式的不同荧光染料：其中n为1,2或3; Z 1和Z 2独立地表示完成苯基或萘基环系所需的碳原子; R 1和R 2中的一个是目标键合基团; 剩余的基团R1或R2选自 - （CH2）4-W或 - （CH2）r-H; R 3是氢，除了当R 1或R 2是 - （CH 2）r-H时，其中R 3是W; W选自磺酸和磺酸盐。 本发明还提供了用荧光染料饱和标记蛋白质的方法，以便标记蛋白质中的所有可用的靶氨基酸，适当的半胱氨酸残基，由此得到单个标记的蛋白质分子群。

公开(公告)号：US06605611B2

公开(公告)日：2003-08-12

申请号：US10233993

申请日：2002-09-03

Applicant: Adrian Christopher Simmonds ,  Alan Hamilton ,  Clifford Smith ,  David Loakes ,  Daniel Brown ,  Fergal Hill ,  Shiv Kumar ,  Satyam Nampalli ,  Mark McDougall

Inventor： Adrian Christopher Simmonds ,  Alan Hamilton ,  Clifford Smith ,  David Loakes ,  Daniel Brown ,  Fergal Hill ,  Shiv Kumar ,  Satyam Nampalli ,  Mark McDougall

IPC: A61K31495

CPC classification number: G01N33/5308 ,  C07H21/00

Abstract: Nucleoside analogues or base analogues having structure (I), where X=O or NH or S, Y=N or CHR6 or CR6, W=N or NR6 or CHR6 or CR6 or S, n=1 or 2; each R6 is independently H or O or alkyl or alkenyl or alkoxy or aryl or a reporter moiety; where necessary (i.e. when Y and/or W is N or CR6 where R6 is not O) a double bond is present between Y and W or W and W, and Q is H or a sugar or a sugar analogue or a nucleic acid backbone or backbone analogue.

Abstract translation: 具有结构（I）的核苷类似物或碱基类似物，其中X = O或NH或S，Y = N或CHR 6或CR 6，W = N或NR 6或CHR 6或CR 6或S，n = 1或2; 每个R 6独立地为H或O或烷基或链烯基或烷氧基或芳基或报道部分; 必要时（即当Y和/或W为N或R6，R6不为O时），Y与W或W和W之间存在双键，Q为H或糖或糖类似物或核酸骨架 或骨架类似物。