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    Polymeric electrode for electrophysiological testing
    3.
    发明授权
    Polymeric electrode for electrophysiological testing 失效
    用于电生理测试的聚合物电极

    公开(公告)号：US06899800B2

    公开(公告)日：2005-05-31

    申请号：US10176183

    申请日：2002-06-20

    申请人： Yuri Osipchuk ,  Alexei Savtchenko ,  Alexander A. Dromaretsky

    发明人： Yuri Osipchuk ,  Alexei Savtchenko ,  Alexander A. Dromaretsky

    IPC分类号： B01L3/00 ,  G01N33/487 ,  G01N27/327 ,  C12M1/34 ,  C12N13/00

    CPC分类号： G01N33/48728 ,  B01L3/5085

    摘要： A polymeric material such as PDMS is molded into an electrode structure containing a micron-size aperture for receiving and forming a giga-ohm seal with a biological membrane. One end of a tube is filled with uncured polymeric material and pressed against a support surface to prevent drainage. A conventional micropipette having a size suitable for sliding through the tube is introduced, tip first, into the tube and is allowed to fall through the polymeric material and rest against the support surface. The assembly is heated to cure the polymer and the micropipette is removed from the tube, thereby leaving a polymeric plug at the end of the tube with an aperture suitable in shape and size for patch-clamp giga-ohm seal electrode applications. A multi-well tray with a polymeric electrode plug in each well is constructed using the same approach.

    摘要翻译： 诸如PDMS的聚合物材料被模制成包含微米级孔径的电极结构，用于接收并用生物膜形成千兆欧密封。 管的一端填充有未固化的聚合物材料并压靠支撑表面以防止排水。 将具有适合于穿过管滑动的尺寸的常规微量移液管首先引入管中并且允许其通过聚合物材料落下并靠在支撑表面上。 将组件加热以固化聚合物，并从管中移除微量移液管，从而在管的端部留下聚合物塞，其孔适于用于膜片钳千兆欧密封电极应用的形状和尺寸。 使用相同的方法构建在每个孔中具有聚合物电极塞的多孔盘。