公开(公告)号：US07699980B2

公开(公告)日：2010-04-20

申请号：US12117785

申请日：2008-05-09

申请人： Richard A. Novak ,  Malcolm Fabiyi ,  Amitabh Gupta

发明人： Richard A. Novak ,  Malcolm Fabiyi ,  Amitabh Gupta

IPC分类号： C02F3/00

CPC分类号： C02F3/12 ,  B01F3/04609 ,  B01F13/0049 ,  C02F3/16 ,  C02F3/206 ,  C02F3/26 ,  C02F2209/001 ,  C02F2209/10 ,  C02F2209/22 ,  Y02W10/15

摘要： An activated sludge wastewater treatment system is provided. The disclosed system includes: an uncovered activated sludge basin; a high purity oxygen aeration system; a secondary clarifier; a return activated sludge line and a waste activated sludge line; wherein the system operates at a solids loading of between about 3000 mg and about 10000 mg of suspended solids per liter of mixed liquor; a dissolved oxygen level in the aeration basin in the range of between about 5 mg to 15 mg; and a solids retention time of between about 7 days and 40 days.

摘要翻译： 提供了一种活性污泥废水处理系统。 所公开的系统包括：未被覆盖的活性污泥池; 高纯度氧气曝气系统; 次级澄清器 回收活性污泥生产线和废活性污泥生产线; 其中所述系统以每升混合液体约3000mg至约10000mg悬浮固体之间的固体负载运行; 在曝气池中的溶解氧水平在约5mg至15mg之间的范围内; 固体保留时间为约7天至40天。

公开(公告)号：US20070148070A1

公开(公告)日：2007-06-28

申请号：US11315065

申请日：2005-12-23

申请人： Shrikar Chakravarti ,  Amitabh Gupta ,  Kenneth Burgers

发明人： Shrikar Chakravarti ,  Amitabh Gupta ,  Kenneth Burgers

IPC分类号： B01D53/14

CPC分类号： B01D53/1456 ,  Y02C10/06

摘要： A gaseous product stream having a predetermined carbon dioxide content of 10-95 mol. % is produced from a gaseous feed stream containing 3-20 mol. % carbon dioxide by converting a portion of the feed stream to a purified stream containing 95-99.9 mol. % (dry basis) carbon dioxide and adding the purified stream to the remaining portion of the feed stream. Alternately, a portion of the purified stream is stored and added subsequently in response to fluctuating demand for the product stream.

摘要翻译： 具有10-95mol的预定二氧化碳含量的气态产物流。 ％由含有3-20mol的气态进料流产生。 通过将一部分进料流转化成含有95-99.9mol的纯化物流来生成二氧化碳。 ％（干基）二氧化碳，并将纯化的流加入进料流的剩余部分。 或者，一部分纯化的流被储存并随后响应于产品流的波动需求而添加。

公开(公告)号：US20070148069A1

公开(公告)日：2007-06-28

申请号：US11315019

申请日：2005-12-23

申请人： Shrikar Chakravarti ,  Kenneth Burgers ,  Amitabh Gupta ,  William Williams

发明人： Shrikar Chakravarti ,  Kenneth Burgers ,  Amitabh Gupta ,  William Williams

IPC分类号： B01D53/14

CPC分类号： B01D53/1493 ,  B01D53/1475 ,  C01B32/50 ,  Y02C10/06 ,  Y02P20/152

摘要： Carbon dioxide is recovered in concentrated form from a gas feed stream also containing oxygen by absorbing carbon dioxide and oxygen into an amine solution that also contains another organic component, removing oxygen, and recovering carbon dioxide from the absorbent.

摘要翻译： 通过将二氧化碳和氧气吸收到还含有另一种有机成分，除去氧气和从吸收剂中回收二氧化碳的胺溶液中，还从含氧气体的气体进料流中浓缩回收二氧化碳。

公开(公告)号：US6146603A

公开(公告)日：2000-11-14

申请号：US329278

申请日：1999-06-10

申请人： Shrikar Chakravarti ,  Amitabh Gupta

发明人： Shrikar Chakravarti ,  Amitabh Gupta

IPC分类号： B01D53/34 ,  B01D19/00 ,  B01D53/14 ,  B01D53/50 ,  B01D53/52 ,  B01D53/62 ,  B01D53/77 ,  B01J20/22 ,  B01J20/34 ,  C01B32/50

CPC分类号： B01D53/1493 ,  B01D53/1418 ,  B01D53/1475 ,  Y02C10/06

摘要： A system for recovering absorbate such as carbon dioxide using an alkanolamine absorbent fluid, wherein the loaded absorbent is heated in a two step heating procedure prior to the separation of the absorbate from the absorbent, and wherein the loaded absorbent is deoxygenated after the first heating step and prior to the second heating step.

摘要翻译： 一种使用链烷醇胺吸收剂流体回收吸收剂如二氧化碳的系统，其中在将吸收物质与吸收剂分离之前，加载的吸收剂在两步加热过程中被加热，并且其中负载的吸收剂在第一加热步骤之后脱氧 并且在第二加热步骤之前。

公开(公告)号：US08241904B2

公开(公告)日：2012-08-14

申请号：US12536559

申请日：2009-08-06

申请人： Alan T. Y. Cheng ,  Ying Zhou ,  Balazs Hunek ,  Amitabh Gupta ,  Nigel Grinter

发明人： Alan T. Y. Cheng ,  Ying Zhou ,  Balazs Hunek ,  Amitabh Gupta ,  Nigel Grinter

IPC分类号： C12N5/02

CPC分类号： C12N1/38 ,  C12M27/02 ,  C12M29/22

摘要： A system and method for controlling a mammalian cell culture process are provided. Such control of the cell culture process involves control of the level of dissolved carbon dioxide in the cell culture media and the resulting ability to prevent increases in the osmolality level is achieved by enhanced stripping of carbon dioxide with little or no damage to the mammalian cells. The disclosed methods and systems of dissolved carbon dioxide stripping include enhanced surface gas exchange mechanisms within the bioreactor vessel through the use of an upward flow impeller combined with vertical baffles to convert swirling motions of the liquid into a largely vertical flow.

摘要翻译： 提供了一种用于控制哺乳动物细胞培养方法的系统和方法。 细胞培养过程的这种控制涉及控制细胞培养基中溶解的二氧化碳的水平，并且通过增强对二氧化碳的去除而对哺乳动物细胞几乎没有或没有损伤来实现所产生的渗透压浓度增加的能力。 所公开的溶解二氧化碳汽提方法和系统包括通过使用与垂直挡板结合的向上流动叶轮将生物反应器容器内的表面气体交换机制改进为将液体的旋转运动转化成大体垂直的流动。

公开(公告)号：US08178318B2

公开(公告)日：2012-05-15

申请号：US12702428

申请日：2010-02-09

申请人： Alan T. Y. Cheng ,  Ying Zhou ,  Amitabh Gupta ,  Balazs Hunek ,  Nigel Grinter

发明人： Alan T. Y. Cheng ,  Ying Zhou ,  Amitabh Gupta ,  Balazs Hunek ,  Nigel Grinter

IPC分类号： C12P21/02

CPC分类号： C12N5/0018 ,  C12N1/38 ,  C12N2500/02 ,  C12N2500/60

摘要： Methods for controlling the level of dissolved carbon dioxide and limiting osmolality in a mammalian cell culture process to enhance cell growth, viability and density, and increase biologic product concentration and yield are provided. Such control of the level of dissolved carbon dioxide and pH as well as the resulting ability to limit osmolality in a mammalian cell culture process is achieved by adopting alternative pH control strategies and CO2 stripping techniques during a mammalian cell culture process. Such pH control techniques and carbon dioxide stripping occur without foam and with little or no damage to the mammalian cells.

摘要翻译： 提供了用于在哺乳动物细胞培养过程中控制溶解二氧化碳水平和限制重量克分子渗透浓度以增强细胞生长，活力和密度以及提高生物产物浓度和产量的方法。 通过在哺乳动物细胞培养过程中采用替代的pH控制策略和CO 2汽提技术来实现对哺乳动物细胞培养过程中溶解的二氧化碳和pH的水平的控制以及所产生的限制重量克分子渗透浓度的能力。 这种pH控制技术和二氧化碳汽提不发生泡沫，对哺乳动物细胞几乎没有或没有损害。

公开(公告)号：US20100184147A1

公开(公告)日：2010-07-22

申请号：US12702428

申请日：2010-02-09

申请人： ALAN T.Y. CHENG ,  Ying Zhou ,  Amitabh Gupta ,  Balazs Hunek ,  Nigel Grinter

发明人： ALAN T.Y. CHENG ,  Ying Zhou ,  Amitabh Gupta ,  Balazs Hunek ,  Nigel Grinter

IPC分类号： C12P21/06 ,  C12N5/02

CPC分类号： C12N5/0018 ,  C12N1/38 ,  C12N2500/02 ,  C12N2500/60

摘要： Methods for controlling the level of dissolved carbon dioxide and limiting osmolality in a mammalian cell culture process to enhance cell growth, viability and density, and increase biologic product concentration and yield are provided. Such control of the level of dissolved carbon dioxide and pH as well as the resulting ability to limit osmolality in a mammalian cell culture process is achieved by adopting alternative pH control strategies and CO2 stripping techniques during a mammalian cell culture process. Such pH control techniques and carbon dioxide stripping occur without foam and with little or no damage to the mammalian cells.

摘要翻译： 提供了用于在哺乳动物细胞培养过程中控制溶解二氧化碳水平和限制重量克分子渗透浓度以增强细胞生长，活力和密度以及提高生物产物浓度和产量的方法。 通过在哺乳动物细胞培养过程中采用替代的pH控制策略和CO 2汽提技术来实现对哺乳动物细胞培养过程中溶解的二氧化碳和pH的水平的控制以及所产生的限制重量克分子渗透浓度的能力。 这种pH控制技术和二氧化碳汽提不发生泡沫，对哺乳动物细胞几乎没有或没有损害。

公开(公告)号：US07699986B2

公开(公告)日：2010-04-20

申请号：US12117791

申请日：2008-05-09

申请人： Richard A. Novak ,  Malcolm Fabiyi ,  Amitabh Gupta

发明人： Richard A. Novak ,  Malcolm Fabiyi ,  Amitabh Gupta

IPC分类号： C02F3/00

CPC分类号： C02F3/12 ,  B01F3/04609 ,  B01F13/0049 ,  C02F3/16 ,  C02F3/206 ,  C02F3/26 ,  C02F2209/001 ,  C02F2209/10 ,  C02F2209/22 ,  Y02W10/15

摘要： An activated sludge wastewater treatment method is provided. The disclosed method includes: receiving a stream of wastewater into a mixed liquor in an uncovered activated sludge basin; introducing high purity oxygen to the mixed liquor in the activated sludge basin to produce euoxic conditions (i.e. a dissolved oxygen level in the range of between about 5 mg to 15 mg) in the activated sludge basin; passing a portion of the mixed liquor in the activated sludge basin through a clarifier to separate activated sludge and produce an effluent stream and an activated sludge stream; and recycling a portion of the activated sludge stream to the activated sludge basin to produce a mixed liquor having a solids loading of between about 3000 mg and about 10000 mg of suspended solids per liter of mixed liquor and a solids retention time of between about 7 days and 40 days.

摘要翻译： 提供了一种活性污泥废水处理方法。 所公开的方法包括：将废水流接收到未覆盖的活性污泥池中的混合液中; 向活性污泥池中的混合液中引入高纯度的氧气，以产生活性污泥池中的无氧条件（即溶解氧水平在约5mg至15mg之间）; 将活性污泥池中的一部分混合液通过澄清器分离出活性污泥并产生流出物流和活性污泥流; 并将活性污泥流的一部分再循环到活性污泥池中以产生每升混合液体的固体负载量为约3000mg至约10000mg悬浮固体和约7天之间的固体保留时间的混合液 和40天。

公开(公告)号：US20100035342A1

公开(公告)日：2010-02-11

申请号：US12536577

申请日：2009-08-06

申请人： ALAN T.Y. CHENG ,  YING ZHOU ,  AMITABH GUPTA ,  BALAZS HUNEK ,  NIGEL GRINTER

发明人： ALAN T.Y. CHENG ,  YING ZHOU ,  AMITABH GUPTA ,  BALAZS HUNEK ,  NIGEL GRINTER

IPC分类号： C12N5/02

CPC分类号： C12N1/38 ,  C12M27/02 ,  C12M29/22

摘要： Methods for controlling the level of dissolved carbon dioxide and limiting osmolality in a mammalian cell culture process to enhance cell growth, viability and density, and increase biologic product concentration and yield are provided. Such control of the level of dissolved carbon dioxide and pH as well as the resulting ability to limit osmolality in a mammalian cell culture process is achieved by adopting alternative pH control strategies and CO2 stripping techniques during a mammalian cell culture process. Such pH control techniques and carbon dioxide stripping occur with little or no damage to the mammalian cells.

摘要翻译： 提供了用于在哺乳动物细胞培养过程中控制溶解二氧化碳水平和限制重量克分子渗透浓度以增强细胞生长，活力和密度以及提高生物产物浓度和产量的方法。 通过在哺乳动物细胞培养过程中采用替代的pH控制策略和CO 2汽提技术来实现对哺乳动物细胞培养过程中溶解的二氧化碳和pH的水平的控制以及所产生的限制重量克分子渗透浓度的能力。 这种pH控制技术和二氧化碳汽提产生对哺乳动物细胞几乎没有或没有损伤。

公开(公告)号：US20090050552A1

公开(公告)日：2009-02-26

申请号：US12117785

申请日：2008-05-09

申请人： Richard A. Novak ,  Malcolm Fabiyi ,  Amitabh Gupta

发明人： Richard A. Novak ,  Malcolm Fabiyi ,  Amitabh Gupta

IPC分类号： C02F3/12

CPC分类号： C02F3/12 ,  B01F3/04609 ,  B01F13/0049 ,  C02F3/16 ,  C02F3/206 ,  C02F3/26 ,  C02F2209/001 ,  C02F2209/10 ,  C02F2209/22 ,  Y02W10/15

摘要： An activated sludge wastewater treatment system is provided. The disclosed system includes: an uncovered activated sludge basin; a high purity oxygen aeration system; a secondary clarifier; a return activated sludge line and a waste activated sludge line; wherein the system operates at a solids loading of between about 3000 mg and about 10000 mg of suspended solids per liter of mixed liquor; a dissolved oxygen level in the aeration basin in the range of between about 5 mg to 15 mg; and a solids retention time of between about 7 days and 40 days.

摘要翻译： 提供了一种活性污泥废水处理系统。 所公开的系统包括：未被覆盖的活性污泥池; 高纯度氧气曝气系统; 次级澄清器 回收活性污泥生产线和废活性污泥生产线; 其中所述系统以每升混合液体约3000mg至约10000mg悬浮固体之间的固体负载运行; 在曝气池中的溶解氧水平在约5mg至15mg之间的范围内; 固体保留时间为约7天至40天。