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1.发明授权Bioreactor with upward flowing impeller system for use in a mammalian cell culture process 有权具有向上流动的叶轮系统的生物反应器用于哺乳动物细胞培养过程公开(公告)号：US09181521B2
公开(公告)日：2015-11-10
申请号：US13559957
申请日：2012-07-27
申请人： Alan T. Y. Cheng , Ying Zhou , Amitabh Gupta , Balazs Hunek , Nigel Grinter
发明人： Alan T. Y. Cheng , Ying Zhou , Amitabh Gupta , Balazs Hunek , Nigel Grinter
CPC分类号： C12M27/20 , C12M27/02 , C12M27/24 , C12M29/06 , C12M41/12 , C12M41/26 , C12M41/32 , C12M41/48 , C12N1/38
摘要： A bioreactor with an upward flowing impeller system for controlling a mammalian cell culture process is provided. The disclosed system enables control of the cell culture process by controlling the level of dissolved carbon dioxide in the cell culture media and prevent increases in the osmolality level is achieved by enhanced stripping of carbon dioxide via surface gas exchange with little or no damage to the mammalian cells. The use of an upward flow impeller combined with vertical baffles converts the swirling motions of the cell culture media into a largely vertical flow and promotes the removal of dissolved carbon dioxide via surface gas exchange with a sweep gas flowing over the top surface of the cell culture media within the bioreactor vessel.
摘要翻译： 提供了一种具有向上流动的叶轮系统用于控制哺乳动物细胞培养过程的生物反应器。 所公开的系统能够通过控制细胞培养基中溶解的二氧化碳的水平来控制细胞培养过程，并且通过经由表面气体交换增强汽提二氧化碳来实现渗透压浓度的增加，对哺乳动物几乎没有或没有损伤 细胞。 使用与垂直挡板结合的向上流动叶轮将细胞培养基的旋转运动转化为大体垂直的流动，并通过与流过细胞培养物顶表面的吹扫气体的表面气体交换促进去除溶解的二氧化碳 生物反应器容器内的介质。
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2.发明申请BIOREACTOR WITH UPWARD FLOWING IMPELLER SYSTEM FOR USE IN A MAMMALIAN CELL CULTURE PROCESS 有权具有向上流动的叶轮系统的生物反应器在马马里细胞培养过程中使用公开(公告)号：US20130189767A1
公开(公告)日：2013-07-25
申请号：US13559957
申请日：2012-07-27
申请人： Alan T. Y. Cheng , Ying Zhou , Amitabh Gupta , Balazs Hunek , Nigel Grinter
发明人： Alan T. Y. Cheng , Ying Zhou , Amitabh Gupta , Balazs Hunek , Nigel Grinter
IPC分类号： C12M1/00
CPC分类号： C12M27/20 , C12M27/02 , C12M27/24 , C12M29/06 , C12M41/12 , C12M41/26 , C12M41/32 , C12M41/48 , C12N1/38
摘要： A bioreactor with an upward flowing impeller system for controlling a mammalian cell culture process is provided. The disclosed system enables control of the cell culture process by controlling the level of dissolved carbon dioxide in the cell culture media and prevent increases in the osmolality level is achieved by enhanced stripping of carbon dioxide via surface gas exchange with little or no damage to the mammalian cells. The use of an upward flow impeller combined with vertical baffles converts the swirling motions of the cell culture media into a largely vertical flow and promotes the removal of dissolved carbon dioxide via surface gas exchange with a sweep gas flowing over the top surface of the cell culture media within the bioreactor vessel.
摘要翻译： 提供了一种具有向上流动的叶轮系统用于控制哺乳动物细胞培养过程的生物反应器。 所公开的系统能够通过控制细胞培养基中溶解的二氧化碳的水平来控制细胞培养过程，并且通过经由表面气体交换增强汽提二氧化碳来实现渗透压浓度的增加，对哺乳动物几乎没有或没有损伤 细胞。 使用与垂直挡板结合的向上流动叶轮将细胞培养基的旋转运动转化为大体垂直的流动，并通过与流过细胞培养物顶表面的吹扫气体的表面气体交换促进去除溶解的二氧化碳 生物反应器容器内的介质。
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公开(公告)号：US08241904B2
公开(公告)日：2012-08-14
申请号：US12536559
申请日：2009-08-06
申请人： Alan T. Y. Cheng , Ying Zhou , Balazs Hunek , Amitabh Gupta , Nigel Grinter
发明人： Alan T. Y. Cheng , Ying Zhou , Balazs Hunek , Amitabh Gupta , Nigel Grinter
IPC分类号： C12N5/02
摘要： A system and method for controlling a mammalian cell culture process are provided. Such control of the cell culture process involves control of the level of dissolved carbon dioxide in the cell culture media and the resulting ability to prevent increases in the osmolality level is achieved by enhanced stripping of carbon dioxide with little or no damage to the mammalian cells. The disclosed methods and systems of dissolved carbon dioxide stripping include enhanced surface gas exchange mechanisms within the bioreactor vessel through the use of an upward flow impeller combined with vertical baffles to convert swirling motions of the liquid into a largely vertical flow.
摘要翻译： 提供了一种用于控制哺乳动物细胞培养方法的系统和方法。 细胞培养过程的这种控制涉及控制细胞培养基中溶解的二氧化碳的水平，并且通过增强对二氧化碳的去除而对哺乳动物细胞几乎没有或没有损伤来实现所产生的渗透压浓度增加的能力。 所公开的溶解二氧化碳汽提方法和系统包括通过使用与垂直挡板结合的向上流动叶轮将生物反应器容器内的表面气体交换机制改进为将液体的旋转运动转化成大体垂直的流动。
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4.发明授权Method for controlling pH, osmolality and dissolved carbon dioxide levels in a mammalian cell culture process to enhance cell viability and biologic product yield 有权用于在哺乳动物细胞培养过程中控制pH，重量摩尔渗透压浓度和溶解二氧化碳水平以增强细胞活力和生物产物产率的方法公开(公告)号：US08178318B2
公开(公告)日：2012-05-15
申请号：US12702428
申请日：2010-02-09
申请人： Alan T. Y. Cheng , Ying Zhou , Amitabh Gupta , Balazs Hunek , Nigel Grinter
发明人： Alan T. Y. Cheng , Ying Zhou , Amitabh Gupta , Balazs Hunek , Nigel Grinter
IPC分类号： C12P21/02
CPC分类号： C12N5/0018 , C12N1/38 , C12N2500/02 , C12N2500/60
摘要： Methods for controlling the level of dissolved carbon dioxide and limiting osmolality in a mammalian cell culture process to enhance cell growth, viability and density, and increase biologic product concentration and yield are provided. Such control of the level of dissolved carbon dioxide and pH as well as the resulting ability to limit osmolality in a mammalian cell culture process is achieved by adopting alternative pH control strategies and CO2 stripping techniques during a mammalian cell culture process. Such pH control techniques and carbon dioxide stripping occur without foam and with little or no damage to the mammalian cells.
摘要翻译： 提供了用于在哺乳动物细胞培养过程中控制溶解二氧化碳水平和限制重量克分子渗透浓度以增强细胞生长，活力和密度以及提高生物产物浓度和产量的方法。 通过在哺乳动物细胞培养过程中采用替代的pH控制策略和CO 2汽提技术来实现对哺乳动物细胞培养过程中溶解的二氧化碳和pH的水平的控制以及所产生的限制重量克分子渗透浓度的能力。 这种pH控制技术和二氧化碳汽提不发生泡沫，对哺乳动物细胞几乎没有或没有损害。
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公开(公告)号：US5540057A
公开(公告)日：1996-07-30
申请号：US497656
申请日：1995-06-30
申请人： Alan T. Y. Cheng
发明人： Alan T. Y. Cheng
CPC分类号： B01D8/00 , Y10S62/903
摘要： A vent gas stream containing volatile organic compounds is passed continuously through a condenser in counter-current flow with respect to cold clean vent gas combined with a cryogenic refrigerant. The volatile organic compounds are condensed at various temperatures inside the condenser, and are separately recovered at very low levels of refrigerant utilization.
摘要翻译： 含有挥发性有机化合物的排放气流相对于与低温制冷剂结合的冷净化排放气体以逆流流动连续通过冷凝器。 挥发性有机化合物在冷凝器内的各种温度下冷凝，并以非常低的制冷剂使用水平单独回收。
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6.发明授权Method of recovering waste heat from edible oil deodorizer and improving product stability 失效从食用油除臭剂中回收废热的方法，提高产品的稳定性
公开(公告)号：US5401866A
公开(公告)日：1995-03-28
申请号：US178980
申请日：1994-01-07
摘要： The invention relates to a process for recovering heat from deodorized edible oils and stabilizing the same comprising: introducing or injecting non-condensible inert gas into hot deodorized edible oil as the hot deodorized edible oil is cooled by indirectly heat exchanging with crude oil to be deodorized.
摘要翻译： 本发明涉及一种从除臭食用油中回收热量并使其稳定化的方法，包括：将不可冷凝惰性气体引入或注入热除臭食用油中，因为热除臭食用油通过与要除臭的原油进行间接热交换而冷却 。
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公开(公告)号：US5634355A
公开(公告)日：1997-06-03
申请号：US522446
申请日：1995-08-31
申请人： Alan T. Y. Cheng , Deepak Lumba
发明人： Alan T. Y. Cheng , Deepak Lumba
CPC分类号： B01D5/0087 , B01D5/0027 , Y10S62/906
摘要： A system for the recovery of volatile compounds comprising a direct contact condenser and an indirect heat exchanger, wherein the heat exchanger is driven by cryogenic fluid, and a heat exchange fluid, having the same composition as the volatile compound, recirculates between the condenser and the heat exchanger.
摘要翻译： 用于回收包含直接接触冷凝器和间接热交换器的挥发性化合物的系统，其中热交换器由低温流体驱动，并且具有与挥发性化合物相同组成的热交换流体在冷凝器和 热交换器。
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公开(公告)号：US5422044A
公开(公告)日：1995-06-06
申请号：US232983
申请日：1994-04-25
申请人： Alan T. Y. Cheng
发明人： Alan T. Y. Cheng
CPC分类号： B01F3/04517 , B01F15/00525 , B01F15/065 , B01F15/005 , B01F2015/062
摘要： A gas injection and heating device and method for the bubbling of a gas into a body of hot liquid to be interacted with the gas. The device comprises an elongate heat exchange gas container, designed to be immersed in the hot liquid to pre-heat the gas in situ by heat exchange with the liquid. Cold gas is supplied to the elongate gas container, circulated therethrough to become heated to the liquid temperature, and then released from a nozzle into the depth of the liquid in the form of small bubbles of hot gas having a large liquid interfacial mass transfer area.
摘要翻译： 一种用于将气体鼓泡到与液体相互作用的热液体中的气体注入和加热装置和方法。 该装置包括细长的热交换气体容器，设计成浸入热液体中以通过与液体的热交换来原位预热气体。 将冷气供应到细长气体容器中，循环通过其中以加热至液体温度，然后以具有大的液体界面传质面积的热气泡形式从喷嘴释放到液体的深度。
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9.发明授权Deodorizing edible oil and/or with non-condensible inert gas and recovering a high quality fatty acid distillate 失效除臭食用油和/或不可冷凝的惰性气体，并回收高品质的脂肪酸馏出物
公开(公告)号：US5374751A
公开(公告)日：1994-12-20
申请号：US79590
申请日：1993-06-22
CPC分类号： C11B3/14
摘要： The invention relates to a process for deodorizing edible oils and/or fats comprising: heating edible oil and/or fat to an elevated temperature; introducing or injecting non-condensible inert gas into said edible oil and/or fat to strip or remove substances that impart disagreeable odor and taste to said edible oil and/or fat; and recovering the resulting deodorized edible oil and/or fat, wherein an amount of said non-condensible inert gas introduced or injected is substantially less than the theoretically required amount for deodorizing said edible oil and/or fat. The condensible gas may be preheated before its introduction into the edible oil and/or fat.
摘要翻译： 本发明涉及一种除臭食用油和/或脂肪的方法，包括：将食用油和/或脂肪加热到升高的温度; 将不可冷凝的惰性气体引入或注入所述食用油和/或脂肪中以剥去或除去对所述食用油和/或脂肪产生不愉快的气味和味道的物质; 并回收所得到的除臭食用油和/或脂肪，其中引入或注入的所述不可冷凝惰性气体的量基本上小于用于对所述食用油和/或脂肪进行除臭的理论要求量。 可冷凝气体可以在其引入食用油和/或脂肪之前被预热。
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10.发明授权Method of recovering waste heat from edible oil deodorizer and improving product stability 失效从食用油除臭剂中回收废热的方法，提高产品的稳定性
公开(公告)号：US5315020A
公开(公告)日：1994-05-24
申请号：US921146
申请日：1992-07-29
摘要： The invention relates to a process for recovering heat from deodorized edible oils and stabilizing the same comprising: introducing or injecting non-condensible inert gas into hot deodorized edible oil as the hot deodorized edible oil is cooled by indirectly heat exchanging with crude oil to be deodorized.
摘要翻译： 本发明涉及一种从除臭食用油中回收热量并使其稳定化的方法，包括：将不可冷凝惰性气体引入或注入热除臭食用油中，因为热除臭食用油通过与要除臭的原油进行间接热交换而冷却 。
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