公开(公告)号：US20150160217A1

公开(公告)日：2015-06-11

申请号：US14376837

申请日：2011-04-12

申请人： Chi-Huey Wong ,  Chung-Yi Wu ,  Chi-Hui Liang ,  An-Suei Yang

发明人： Chi-Huey Wong ,  Chung-Yi Wu ,  Chi-Hui Liang ,  An-Suei Yang

IPC分类号： G01N33/569

CPC分类号： G01N33/56983 ,  G01N33/53 ,  G01N33/54346 ,  G01N2333/11 ,  G01N2400/00 ,  G01N2400/38 ,  G01N2458/00

摘要： Glycan arrays that can detect and distinguish between various sub-types and strains of influenza virus are provided. Methods for using the glycan arrays with assays using nanoparticle amplification technique are disclosed. Sandwich assays using gold nanoparticles conjugated to phage particles comprising influenza virus-specific antibodies for detecting multiple serotypes using a single reaction are provided. Plurality of glycans directed to specific target HA of influenza virus comprises the array. Detector molecules comprising noble metals conjugated to (a) phage display particles expressing antibodies against hemagglutinin and (b) neuraminidase binding agents are disclosed.

摘要翻译： 提供了可以检测和区分各种亚型和流感病毒株的糖阵列。 公开了使用纳米颗粒扩增技术的测定法使用聚糖阵列的方法。 提供使用与包含用于使用单个反应检测多种血清型的流感病毒特异性抗体的噬菌体颗粒缀合的金纳米颗粒的三明治测定。 针对流感病毒的特定目标HA的多种聚糖包括阵列。 公开了包含与（a）噬菌体缀合的贵金属的检测分子，其显示表达针对血凝素的抗体的颗粒和（b）神经氨酸酶结合剂。

公开(公告)号：US10338069B2

公开(公告)日：2019-07-02

申请号：US14376837

申请日：2011-04-12

申请人： Chi-Huey Wong ,  Chung-Yi Wu ,  Chi-Hui Liang ,  An-Suei Yang

发明人： Chi-Huey Wong ,  Chung-Yi Wu ,  Chi-Hui Liang ,  An-Suei Yang

IPC分类号： G01N33/569 ,  G01N33/53 ,  G01N33/543

摘要： Glycan arrays that can detect and distinguish between various sub-types and strains of influenza virus are provided. Methods for using the glycan arrays with assays using nanoparticle amplification technique are disclosed. Sandwich assays using gold nanoparticles conjugated to phage particles comprising influenza virus-specific antibodies for detecting multiple serotypes using a single reaction are provided. Plurality of glycans directed to specific target HA of influenza virus comprises the array. Detector molecules comprising noble metals conjugated to (a) phage display particles expressing antibodies against hemagglutinin and (b) neuraminidase binding agents are disclosed.

公开(公告)号：US20110082052A1

公开(公告)日：2011-04-07

申请号：US12854632

申请日：2010-08-11

申请人： An-Suei Yang ,  Yu-Ching Lee ,  Ing-Chien Chen ,  Chung-Ming Yu ,  Hung-Ju Hsu ,  Yi-Jen Huang ,  Hung-Ju Chang ,  Keng-Chang Tsai ,  Hung-Pin Peng

发明人： An-Suei Yang ,  Yu-Ching Lee ,  Ing-Chien Chen ,  Chung-Ming Yu ,  Hung-Ju Hsu ,  Yi-Jen Huang ,  Hung-Ju Chang ,  Keng-Chang Tsai ,  Hung-Pin Peng

IPC分类号： C40B40/02 ,  C40B40/06 ,  C07H21/04 ,  C12N1/00 ,  C12N7/00 ,  C12P21/00 ,  C40B40/10 ,  C07K14/00 ,  C07H21/00

CPC分类号： C07K16/005 ,  C07K16/1018 ,  C07K16/22 ,  C07K2317/622 ,  C07K2317/624 ,  C07K2319/02 ,  C12N15/1037 ,  C12N15/1051 ,  C12N15/73 ,  C12N2795/00022 ,  C40B40/08 ,  C40B50/06

摘要： Disclosed are nucleic acid libraries for identifying a signal peptide that facilitates production of disulfide-stabilized single chain antibody, and for facilitating production of a disulfide-stabilized single chain antibody. Also disclosed are host cell libraries and phage libraries including the nucleic acid libraries. Further disclosed are methods for identifying a signal peptide that facilitates production of disulfide-stabilized single chain antibody, and methods for producing a disulfide-stabilized single chain antibody and non-fusion form thereof.

摘要翻译： 公开了用于鉴定促进二硫键稳定的单链抗体的产生并促进二硫键稳定的单链抗体的产生的信号肽的核酸文库。 还公开了宿主细胞文库和包括核酸文库的噬菌体文库。 进一步公开了用于鉴定促进二硫键稳定的单链抗体的产生的信号肽的方法，以及用于制备二硫键稳定的单链抗体及其非融合形式的方法。