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公开(公告)号：US09018580B2
公开(公告)日：2015-04-28
申请号：US13496949
申请日：2010-09-15
CPC分类号： G01N33/6848 , H01J49/0031 , H01J49/42 , H01J49/427
摘要： A method for detecting at least one target molecule in a sample by mass spectrometry, comprising ionizing the molecules of the sample and then conducting the following steps (i) and (ii) n times, n being equal to 0, 1, 2, 3 or 4: (i) at least one ion obtained in the preceding step is selected, according to the target molecule, in a mass analyzer, and (ii) the ion thus selected is fragmented in a fragmentation cell; trapping at least two different sampled ions, when n is zero, or when n is other than zero, in a mass analyzer, the at least two ions thus trapped having a mass-to-charge ratio m/z characteristic of the target molecule; ejecting the trapped ions from the mass analyzer; and detecting the ejected ions ejected by means of a detection device. The method is characterized in that the characteristic ions are ejected simultaneously and detected simultaneously.
摘要翻译： 一种用于通过质谱法检测样品中的至少一种靶分子的方法，包括使样品的分子电离，然后进行以下步骤（i）和（ii）n次，n等于0,1,2,3 或4：（i）根据靶分子在质量分析器中选择前述步骤中获得的至少一种离子，和（ii）所选择的离子在碎裂单元中碎裂; 捕获至少两种不同的采样离子，当n为零时，或当n不为零时，在质量分析器中，至少两个离子因此被捕获，其具有靶分子的质荷比m / z特性; 从质量分析器中排出被捕获的离子; 并且检测通过检测装置喷射的喷射离子。 该方法的特征在于同时喷射特征离子并同时检测。
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公开(公告)号：US20120205535A1
公开(公告)日：2012-08-16
申请号：US13496949
申请日：2010-09-15
IPC分类号： H01J49/26
CPC分类号： G01N33/6848 , H01J49/0031 , H01J49/42 , H01J49/427
摘要： A method for detecting at least one target molecule in a sample by mass spectrometry, comprising ionizing the molecules of the sample and then conducting the following steps (i) and (ii) n times, n being equal to 0, 1, 2, 3 or 4: (i) at least one ion obtained in the preceding step is selected, according to the target molecule, in a mass analyser, and (ii) the ion thus selected is fragmented in a fragmentation cell; trapping at least two different sampled ions, when n is zero, or when n is other than zero, in a mass analyser, the at least two ions thus trapped having a mass-to-charge ratio m/z characteristic of the target molecule; ejecting the trapped ions from the mass analyser; and detecting the ejected ions ejected by means of a detection device. The method is characterized in that the characteristic ions are ejected simultaneously and detected simultaneously.
摘要翻译： 一种用于通过质谱法检测样品中的至少一种靶分子的方法，包括使样品的分子电离，然后进行以下步骤（i）和（ii）n次，n等于0,1,2,3 或4：（i）根据靶分子在质量分析器中选择前述步骤中获得的至少一种离子，和（ii）所选择的离子在碎裂单元中碎裂; 捕获至少两种不同的采样离子，当n为零时，或当n不为零时，在质量分析器中，至少两个离子因此被捕获，其具有靶分子的质荷比m / z特性; 从质量分析器中排出被捕获的离子; 并且检测通过检测装置喷射的喷射离子。 该方法的特征在于同时喷射特征离子并同时检测。
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公开(公告)号：US20120208224A1
公开(公告)日：2012-08-16
申请号：US13322173
申请日：2010-05-21
IPC分类号： G01N27/62
CPC分类号： G01N33/6848
摘要： The present invention relates to a method for the quantitative detection of a target protein in a sample, in which the second-generation fragment ions are detected for providing a series of quantitative measurements, at least one of which is correlated to the amount of proteotypic peptide generated and to the amount of target protein in the sample, characterized in that the selected first-generation fragment ion having a mass (m/z)2 is a doubly-charged peptide having a proline and/or a histidine in position 1.
摘要翻译： 本发明涉及用于定量检测样品中靶蛋白的方法，其中检测第二代碎片离子以提供一系列定量测量，其中至少一种与蛋白多肽的量相关 产生和样品中靶蛋白的量，其特征在于所选择的具有质量（m / z）2的第一代碎片离子是位置1具有脯氨酸和/或组氨酸的双电荷肽。
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公开(公告)号：US09170263B2
公开(公告)日：2015-10-27
申请号：US13322173
申请日：2010-05-21
CPC分类号： G01N33/6848
摘要： The present invention relates to a method for the quantitative detection of a target protein in a sample, in which the second-generation fragment ions are detected for providing a series of quantitative measurements, at least one of which is correlated to the amount of proteotypic peptide generated and to the amount of target protein in the sample, characterized in that the selected first-generation fragment ion having a mass (m/z)2 is a doubly-charged peptide having a proline and/or a histidine in position 1.
摘要翻译： 本发明涉及用于定量检测样品中靶蛋白的方法，其中检测第二代碎片离子以提供一系列定量测量，其中至少一种与蛋白多肽的量相关 产生和样品中靶蛋白的量，其特征在于所选择的具有质量（m / z）2的第一代碎片离子是位置1具有脯氨酸和/或组氨酸的双电荷肽。
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