公开(公告)号：US20110201094A1

公开(公告)日：2011-08-18

申请号：US13081096

申请日：2011-04-06

申请人： Arthur M. Nonomura

发明人： Arthur M. Nonomura

IPC分类号： C12N1/12

CPC分类号： C12P5/00 ,  A01H13/00 ,  C12N1/12 ,  C12R1/89

摘要： Acceleration of botryococcenoids and growth by concomitant provision of appropriate light, minerals, and assimilable carbon. Specifically, methods, compositions and systems for the in vitro growth of hydrocarbons in photosynthetic organisms while maintaining a biologically exclusive monocultural environment, as for example, from Botryococcus species, is disclosed. Niche-nutrients can include about 200 ppm to about 3% nitrogen, and about 100 ppm to about 15% P205, and about 100 ppm to about 3.5% K20. In certain embodiments, the present invention relates to the growth of the Chlorophyta such as Botryococcus sp. in a nutrient medium that includes up to 15% phosphates, at least 3 ppm soluble iron, and up to about 70 ppm soluble zinc. Also disclosed is a substantially pure culture of Botryococcus braunii var. Showa, strain Ninsei, having the ATCC Accession No. PTA-7441, its parts, and hydrocarbons produced therefrom.

摘要翻译： 通过伴随提供适当的光，矿物质和可吸收的碳来加速葡萄球菌和生长。 具体地说，公开了用于在光合生物体内体外生长碳氢化合物的方法，组合物和系统，同时保持生物学独特的单一文化环境，例如来自Botryococcus物种。 利基营养素可以包括约200ppm至约3％的氮，以及约100ppm至约15％的P205和约100ppm至约3.5％的K20。 在某些实施方案中，本发明涉及绿藻​​属如Botryococcus sp。 在包含高达15％磷酸盐，至少3ppm可溶性铁和至多约70ppm可溶性锌的营养培养基中。 还公开了一种基本上纯的Botryococcus braunii变种培养物。 昭和菌株Ninsei，具有ATCC登录号PTA-7441，其部分和由其生产的烃。

公开(公告)号：US20090087889A1

公开(公告)日：2009-04-02

申请号：US11919082

申请日：2006-05-05

申请人： Arthur M. Nonomura

发明人： Arthur M. Nonomura

IPC分类号： C12P5/00 ,  C12N5/04 ,  C07C11/00

CPC分类号： C12P5/00 ,  A01H13/00 ,  C12N1/12 ,  C12R1/89

摘要： Bottom dwelling colonies of Botryococcus are stimulated to produce and accumulate unusually high hydrocarbon concentrations that make them float to the surface. When exposed to carbon dioxide gas at the surface of the water, hydrocarbon induced flotation and growth is further enhanced with concomitant provision of appropriate light and available nitrogen. Botryococcus var. Ninsei is distinct from previously cultured strains of the genus in color, metabolism, and niche. The Ninsei variety grows green at the air-water interface. Metabolically, Ninsei is distinct from other varieties because it produces hydrocarbons in the presence of surplus ammonia in contrast to other varieties that shut down hydrocarbon production when ammonia is available. These characteristics are consistent with growth on the surface at water's edge, e.g., in the niche characterized by a mud alga. The surface niche for Ninsei is distinct because all other varieties are submerged, occupying the floor, e.g., all other varieties live at the bottom of the culture. Flotation of green colonies based on concomitant production of hydrocarbons in vitro occurs solely in the Ninsei variety.

摘要翻译： 刺激Botryococcus的底部住宅菌群产生和积累异常高的碳氢化合物浓度，使其浮在表面。 当暴露于水表面的二氧化碳气体时，随着提供适当的光和有效氮，进一步提高烃诱导的浮选和生长。 Botryococcus var。 Ninsei不同于以前培育的颜色，新陈代谢和利基属的菌株。 Ninsei品种在空气 - 水界面生长绿色。 在代谢方面，Ninsei与其他品种不同，因为在存在多余氨的情况下生产碳氢化合物，与其他可用氨气关闭碳氢化合物的品种相反。 这些特征与在水边缘的表面上的生长一致，例如在以泥浆为特征的小生境中。 Ninsei的表面利基是独特的，因为所有其他品种都被淹没，占据了地板，例如所有其他品种都生活在文化的底部。 基于在体外同时生产碳氢化合物的绿色菌落的浮选仅在Ninsei品种中进行。

公开(公告)号：US5962717A

公开(公告)日：1999-10-05

申请号：US265268

申请日：1999-03-09

申请人： Arthur M. Nonomura ,  Barry A. Cullen ,  Joseph J. Crudden ,  John J. Nishio

发明人： Arthur M. Nonomura ,  Barry A. Cullen ,  Joseph J. Crudden ,  John J. Nishio

IPC分类号： C05D9/02 ,  C05G3/00 ,  C07C229/76 ,  C07F3/06 ,  C07F13/00 ,  C07F15/06

CPC分类号： C07C229/76 ,  C05D9/02 ,  C05G3/0064 ,  C05G3/0076 ,  Y10S71/02

摘要： Plant micronutrient compounds comprising N-acyl ED3A or salts thereof. The N-acyl ED3A derivatives chelate various trace metals, including iron, copper, zinc, manganese, cobalt and nickel.

摘要翻译： 包含N-酰基ED3A的植物微量营养素化合物或其盐。 N-酰基ED3A衍生物螯合各种痕量金属，包括铁，铜，锌，锰，钴和镍。

公开(公告)号：USPP6169P

公开(公告)日：1988-05-03

申请号：US852048

申请日：1986-04-15

申请人： Arthur M. Nonomura

发明人： Arthur M. Nonomura

摘要： Botryococcus braunii var. showa is chemotaxonomically distinct from previously cultured strains of the species in quality and quantity of hydrocarbons produced in vitro. Morphological and cultural differences distinguish this variety from other cultured strains of Botryococcus braunii. In particular, the variety is characterized by the ability to produce and secrete large amounts of botryococcenes during all phases of its growth cycle.

公开(公告)号：US20120077679A1

公开(公告)日：2012-03-29

申请号：US13314234

申请日：2011-12-08

申请人： Arthur M. Nonomura

发明人： Arthur M. Nonomura

IPC分类号： A01N55/02 ,  A01P21/00

CPC分类号： A01H5/00 ,  A01N43/16 ,  A01N59/06 ,  C05C9/00 ,  A01N25/00 ,  A01N25/30 ,  A01N37/04 ,  A01N37/44 ,  A01N61/00 ,  A01N2300/00 ,  C05D9/02 ,  C05F11/00

摘要： Methods and compositions comprising design of enlarged pectins are provided. Elaborated pectins are useful for a multitude of functions including surface coating, penetrant, additive and gel. Methods according to the present invention comprise steps for treatments to one or more live pectic cells in one or more compositions that result in modified pectins. In specific embodiments, methods are provided for applying the nanotechnology to live cells for carriage and incorporation of polar compounds.

摘要翻译： 提供包括扩大果胶的设计的方法和组合物。 精心制作的果胶可用于多种功能，包括表面涂层，渗透剂，添加剂和凝胶。 根据本发明的方法包括用于在导致改性果胶的一种或多种组合物中对一种或多种活的果胶细胞进行处理的步骤。 在具体实施方案中，提供了将纳米技术应用于活细胞以运载和掺入极性化合物的方法。

公开(公告)号：US07923228B2

公开(公告)日：2011-04-12

申请号：US11429536

申请日：2006-05-05

申请人： Arthur M. Nonomura

发明人： Arthur M. Nonomura

IPC分类号： C12P5/00

CPC分类号： C12P5/00 ,  A01H13/00 ,  C12N1/12 ,  C12R1/89

摘要： Acceleration of botryococcenoids and growth by concomitant provision of appropriate light, minerals, and assimilable carbon. Specifically, methods, compositions and systems for the in vitro growth of hydrocarbons in photosynthetic organisms while maintaining a biologically exclusive monocultural environment, as for example, from Botryococcus species, is disclosed. Niche-nutrients can include about 200 ppm to about 3% nitrogen, and about 100 ppm to about 15% P2O5, and about 100 ppm to about 3.5% K2O. In certain embodiments, the present invention relates to the growth of the Chlorophyta such as Botryococcus sp. in a nutrient medium that includes up to 15% phosphates, at least 3 ppm soluble iron, and up to about 70 ppm soluble zinc. Also disclosed is a substantially pure culture of Botryococcus braunii var. Showa, strain Ninsei, having the ATCC Accession No. PTA-7441, its parts, and hydrocarbons produced therefrom.

摘要翻译： 通过伴随提供适当的光，矿物质和可吸收的碳来加速葡萄球菌和生长。 具体地说，公开了用于在光合生物体内体外生长碳氢化合物的方法，组合物和系统，同时保持生物学独特的单一文化环境，例如来自Botryococcus物种。 利基营养素可以包括约200ppm至约3％的氮，以及约100ppm至约15％的P2O5和约100ppm至约3.5％的K2O。 在某些实施方案中，本发明涉及绿藻​​属如Botryococcus sp。 在包含高达15％磷酸盐，至少3ppm可溶性铁和至多约70ppm可溶性锌的营养培养基中。 还公开了一种基本上纯的Botryococcus braunii变种培养物。 昭和菌株Ninsei，具有ATCC登录号PTA-7441，其部分和由其生产的烃。

公开(公告)号：USPP21091P3

公开(公告)日：2010-06-22

申请号：US11429531

申请日：2006-05-05

申请人： Arthur M. Nonomura

发明人： Arthur M. Nonomura

IPC分类号： A01H5/00

CPC分类号： C12P5/00 ,  A01H13/00 ,  C12N1/12 ,  C12R1/89

摘要： A novel variety of Botryococcus distinct from the previously cultured variety in color, biochemistry, size, shape, and habit.

摘要翻译： 一种新品种的Botryococcus与以前培育的品种在颜色，生物化学，大小，形状和习惯上不同。

公开(公告)号：US06258749B1

公开(公告)日：2001-07-10

申请号：US09510696

申请日：2000-02-22

申请人： Arthur M. Nonomura

发明人： Arthur M. Nonomura

IPC分类号： A01N4316

CPC分类号： C05F11/10 ,  A01N25/32 ,  A01N43/16 ,  C05D9/00 ,  A01N59/16 ,  A01N59/02 ,  A01N59/00 ,  A01N2300/00 ,  C05F11/00

摘要： Methods and formulations for treating plants and enhancing plant growth and for safening high concentrations of one or more phytocatalysts, wherein one or more formulations, comprising, a high concentration of one or more phytocatalysts, and an effective amount of one or more polyacylglycosides and polyalkylglycosides; and isomers, and metabolites, salts, hydrates, esters, amines, and derivatives of the polyacylglycosides and polyalkylglycosides, and combinations thereof, is applied to the plants.

摘要翻译： 用于处理植物并增强植物生长并用于安全高浓度的一种或多种植物催化剂的方法和制剂，其中一种或多种制剂，包含高浓度的一种或多种植物催化剂和有效量的一种或多种聚酰基甘氨酸和多烷基糖苷; 并且异构体，以及多酰基糖苷类和多烷基糖苷类的代谢物，盐，水合物，酯，胺和衍生物及其组合被施用于植物。

公开(公告)号：US4713398A

公开(公告)日：1987-12-15

申请号：US771401

申请日：1985-08-30

申请人： Arthur M. Nonomura

发明人： Arthur M. Nonomura

IPC分类号： A61K31/07 ,  A61K47/44 ,  C07C403/24 ,  A61K31/355 ,  A61K31/40

CPC分类号： C07C403/24 ,  A61K31/07 ,  A61K47/44 ,  Y10S514/937

摘要： A composition is described comprising naturally-derived carotene dispersed in a non-petroleum, naturally-derived edible oil. The carotene may be in form of its mixed isomers, or one or more of the individual isomers may be present to the exclusion of the others. Of the isomers the beta-carotene isomer is preferred, although others, notably the alpha- and gamma-carotene isomers, are also desirable components. Naturally-derived stabilizers may be present in the composition. The carotene content is 0.5-7.5 weight percent of the composition. The carotene is preferably obtained from algae. No petro-chemical residues are present in the composition since the formulation is produced at all stages without use of petrochemical contact.

摘要翻译： 描述了包含分散在非石油天然来源的食用油中的天然来源的胡萝卜素的组合物。 胡萝卜素可以是其混合异构体的形式，或者可以存在一种或多种单独的异构体以排除其它异构体。 在异构体中，β-胡萝卜素异构体是优选的，尽管其它特别是α和γ-胡萝卜素异构体也是期望的组分。 天然衍生的稳定剂可以存在于组合物中。 胡萝卜素含量为组合物的0.5-7.5重量％。 胡萝卜素优选从藻类获得。 在组合物中不存在石油化学残留物，因为在不使用石油化学接触的情况下在所有阶段生产制剂。

公开(公告)号：US4680314A

公开(公告)日：1987-07-14

申请号：US771402

申请日：1985-08-30

申请人： Arthur M. Nonomura

发明人： Arthur M. Nonomura

IPC分类号： A61K31/07 ,  A61K36/04 ,  A61K36/05 ,  C09B61/00 ,  A61K31/355

CPC分类号： C09B61/00 ,  A61K36/04 ,  A61K36/05 ,  C07C403/24

摘要： A process is described for the formation of a naturally derived composition comprising carotenes, particularly beta-carotene, in oil. The process involves the direct oil extraction of the carotene from algae. The algae are initially harvested as a water slurry and concentrated, as by flocculation, in the slurry. The concentrated portion of the slurry is then mixed with oil and homogenized to form an oil emulsion. Direct contact of the oil and algae results in a high degree of extraction of the carotene from the algae by the oil. The water/oil emulsion is broken and the oil phase containing the carotene separated as by centrifugation and recovered. The resulting carotene/oil composition is useful as a precursor in formation of Vitamin A in human nutrition. The carotene itself may also have some use as a preventative material for certain types of cancers or in supplementation of poultry and livestock feed.

摘要翻译： 描述了一种在油中形成包含胡萝卜素，特别是β-胡萝卜素的天然衍生组合物的方法。 该方法涉及从藻类直接提取胡萝卜素。 藻类首先作为水浆收获，并通过絮凝在浆液中浓缩。 然后将浆料的浓缩部分与油混合并均化以形成油乳液。 油和藻类的直接接触导致通过油从藻类中高度提取胡萝卜素。 水/油乳液破碎，通过离心分离含有胡萝卜素的油相并回收。 所得到的胡萝卜素/油组合物可用作维生素A在人类营养中形成的前体。 胡萝卜素本身也可以作为某些类型的癌症的预防材料或补充家禽和牲畜饲料。