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    Methods and compositions for growth of hydrocarbons in Botryococcus sp
    1.
    发明授权
    Methods and compositions for growth of hydrocarbons in Botryococcus sp 有权
    用于生产Botryococcus sp。的烃的方法和组合物

    公开(公告)号：US07923228B2

    公开(公告)日：2011-04-12

    申请号：US11429536

    申请日：2006-05-05

    申请人： Arthur M. Nonomura

    发明人： Arthur M. Nonomura

    IPC分类号： C12P5/00

    CPC分类号： C12P5/00 ,  A01H13/00 ,  C12N1/12 ,  C12R1/89

    摘要： Acceleration of botryococcenoids and growth by concomitant provision of appropriate light, minerals, and assimilable carbon. Specifically, methods, compositions and systems for the in vitro growth of hydrocarbons in photosynthetic organisms while maintaining a biologically exclusive monocultural environment, as for example, from Botryococcus species, is disclosed. Niche-nutrients can include about 200 ppm to about 3% nitrogen, and about 100 ppm to about 15% P2O5, and about 100 ppm to about 3.5% K2O. In certain embodiments, the present invention relates to the growth of the Chlorophyta such as Botryococcus sp. in a nutrient medium that includes up to 15% phosphates, at least 3 ppm soluble iron, and up to about 70 ppm soluble zinc. Also disclosed is a substantially pure culture of Botryococcus braunii var. Showa, strain Ninsei, having the ATCC Accession No. PTA-7441, its parts, and hydrocarbons produced therefrom.

    摘要翻译： 通过伴随提供适当的光，矿物质和可吸收的碳来加速葡萄球菌和生长。 具体地说，公开了用于在光合生物体内体外生长碳氢化合物的方法，组合物和系统，同时保持生物学独特的单一文化环境，例如来自Botryococcus物种。 利基营养素可以包括约200ppm至约3％的氮，以及约100ppm至约15％的P2O5和约100ppm至约3.5％的K2O。 在某些实施方案中，本发明涉及绿藻​​属如Botryococcus sp。 在包含高达15％磷酸盐，至少3ppm可溶性铁和至多约70ppm可溶性锌的营养培养基中。 还公开了一种基本上纯的Botryococcus braunii变种培养物。 昭和菌株Ninsei，具有ATCC登录号PTA-7441，其部分和由其生产的烃。

    High photoefficiency microalgae bioreactors
    2.
    发明授权
    High photoefficiency microalgae bioreactors 失效
    高光效微藻生物反应器

    公开(公告)号：US07763457B2

    公开(公告)日：2010-07-27

    申请号：US11549552

    申请日：2006-10-13

    申请人： Eric H. Dunlop ,  David A. Hazlebeck

    发明人： Eric H. Dunlop ,  David A. Hazlebeck

    IPC分类号： C12M1/00

    CPC分类号： A01H13/00 ,  C12M21/02 ,  C12M23/06 ,  C12M27/02 ,  C12M43/02 ,  C12M47/02 ,  C12M47/06

    摘要： A system and method are provided for growing algae with improved photoefficiency. The system includes a bioreactor formed with a conduit for growing algae cells in a medium. Further, the system is provided with a paddle wheel for moving the medium through the conduit at a predetermined fluid flow velocity. In order to ensure that algae cells are efficiently converting light energy to chemical energy, a plurality of barriers is positioned in the fluid stream. Specifically, the barriers are separated by predetermined distances to create von Karman vortices in the medium. As a result, algae cells are sequentially flowed to the surface of the fluid stream to receive light energy for predetermined intervals of time.

    摘要翻译： 提供了一种提高光效的藻类生长系统和方法。 该系统包括形成有用于在培养基中生长藻类细胞的导管的生物反应器。 此外，系统设置有用于以预定的流体流动速度移动介质通过导管的桨轮。 为了确保藻类细胞有效地将光能转化为化学能，多个屏障位于流体流中。 具体来说，障碍物被隔开预定的距离，以在介质中产生冯卡曼旋涡。 结果，藻类细胞依次流到流体流的表面，以接收预定时间间隔的光能。

    High photoefficiency microalgae bioreactors
    4.
    发明申请
    High photoefficiency microalgae bioreactors 失效
    高光效微藻生物反应器

    公开(公告)号：US20080086939A1

    公开(公告)日：2008-04-17

    申请号：US11549552

    申请日：2006-10-13

    申请人： Eric H. Dunlop ,  David A. Hazlebeck

    发明人： Eric H. Dunlop ,  David A. Hazlebeck

    IPC分类号： A01H13/00

    CPC分类号： A01H13/00 ,  C12M21/02 ,  C12M23/06 ,  C12M27/02 ,  C12M43/02 ,  C12M47/02 ,  C12M47/06

    摘要： A system and method are provided for growing algae with improved photoefficiency. The system includes a bioreactor formed with a conduit for growing algae cells in a medium. Further, the system is provided with a means for moving the medium through the conduit at a predetermined fluid flow velocity. In order to ensure that algae cells are efficiently converting light energy to chemical energy, a plurality of barriers is positioned in the fluid stream. Specifically, the barriers are separated by predetermined distances to create von Karman vortices in the medium. As a result, algae cells are sequentially flowed to the surface of the fluid stream to receive light energy for predetermined intervals of time.

    摘要翻译： 提供了一种提高光效的藻类生长系统和方法。 该系统包括形成有用于在培养基中生长藻类细胞的导管的生物反应器。 此外，该系统设置有用于以预定的流体流动速度移动介质通过导管的装置。 为了确保藻类细胞有效地将光能转化为化学能，多个屏障位于流体流中。 具体来说，障碍物被隔开预定的距离，以在介质中产生冯卡曼旋涡。 结果，藻类细胞依次流到流体流的表面，以接收预定时间间隔的光能。

    Genetically engineered duckweed
    5.
    发明申请
    Genetically engineered duckweed 审中-公开
    遗传工程浮萍

    公开(公告)号：US20070044177A1

    公开(公告)日：2007-02-22

    申请号：US11590071

    申请日：2006-10-31

    申请人： Anne-Marie Stomp ,  Nirmala Rajbhandari

    发明人： Anne-Marie Stomp ,  Nirmala Rajbhandari

    IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N5/04

    CPC分类号： A01H4/005 ,  A01H13/00 ,  C12N15/8205 ,  C12N15/8207 ,  C12N15/8257

    摘要： Methods and compositions for the efficient transformation of duckweed are provided. Preferably, the methods involve transformation by either ballistic bombardment or Agrobacterium. In this manner, any gene or nucleic acid of interest can be introduced and expressed in duckweed plants. Transformed duckweed plants, cells, tissues are also provided. Transformed duckweed plant tissue culture and methods of producing recombinant proteins and peptides from transformed duckweed plants are also disclosed.

    摘要翻译： 提供了有效转化浮萍的方法和组成。 优选地，所述方法包括通过弹道轰击或农杆菌转化。 以这种方式，可以在浮萍植物中引入和表达任何感兴趣的基因或核酸。 还提供了转化的浮萍植物，细胞，组织。 还公开了转化的浮萍植物组织培养物和从转化的浮萍植物生产重组蛋白质和肽的方法。

    Genetically modified Cyanobacteria for the production of ethanol, the constructs and method thereof
    6.
    发明申请
    Genetically modified Cyanobacteria for the production of ethanol, the constructs and method thereof 有权
    用于生产乙醇的转基因蓝细菌，其构建及其方法

    公开(公告)号：US20020042111A1

    公开(公告)日：2002-04-11

    申请号：US09861819

    申请日：2001-05-21

    申请人： Enol Energy Inc.

    发明人： Robert Paul Woods ,  John Robert Coleman ,  Ming De Deng

    IPC分类号： C12P007/06 ,  C12N001/21

    CPC分类号： C12P7/065 ,  A01H13/00 ,  Y02E50/17

    摘要： The invention provides a genetically modified Cyanobacteria having a construct comprising DNA fragments encoding pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adh) enzymes obtained from the Zymomonas mobilis plasmid pLOI295. The Cyanobacteria are capable of producing ethanol in recoverable quantities of at least 1.7 nullmol ethanol per mg of chlorophyll per hour.

    摘要翻译： 本发明提供了具有构建体的经遗传修饰的蓝细菌，该构建体包含从运动发酵单胞菌素质粒pLOI295获得的编码丙酮酸脱羧酶（pdc）和醇脱氢酶（adh）的DNA片段。 蓝细菌能够以每小时每mg叶绿素的可回收量至少为1.7微摩尔乙醇生产乙醇。

    SYSTEMS AND METHODS FOR TREATING WASTE STREAMS
    9.
    发明公开
    SYSTEMS AND METHODS FOR TREATING WASTE STREAMS 审中-公开

    公开(公告)号：US20230203414A1

    公开(公告)日：2023-06-29

    申请号：US18161324

    申请日：2023-01-30

    申请人： GSR Solutions, LLC

    发明人： Anju D. Krivov

    IPC分类号： C12M1/00 ,  A01H13/00 ,  C12P5/02 ,  C12M1/107 ,  C02F11/02 ,  C02F11/04

    CPC分类号： C12M21/02 ,  A01H13/00 ,  C12P5/02 ,  C12M23/58 ,  C12M47/06 ,  C12M21/04 ,  C12M1/002 ,  C12M43/00 ,  C02F11/02 ,  C02F11/04

    摘要： According to present disclosure, there is disclosed an algae growth and cultivation system that provides a cost-efficient means of producing algae biomass as feedstock for algae-based products, such as, fertilizer, feed, biofuel manufacture, and desirably impacts, nutrient recovery from waste streams for valued byproducts production, recycle water, and alternative/renewable energy production. The system as discussed herein is an integrated systems approach to wastewater treatment, algal strains selection for byproducts production, and recycle of algal biomass-processing waste or additional algae harvested as feedstock for products such as fertilizer production. Embodiments of a system as discussed herein present an economically viable algae production system and process that allows algae-derived products such as fertilizer, feed, biofuels, etc. to compete with non-organic or petroleum products in the marketplace.