公开(公告)号：US20110111446A1

公开(公告)日：2011-05-12

申请号：US12919299

申请日：2009-01-30

申请人： Hiroshi Abe ,  Yoshihiro Ito ,  Aya Shibata ,  Mika Ito

发明人： Hiroshi Abe ,  Yoshihiro Ito ,  Aya Shibata ,  Mika Ito

IPC分类号： C12Q1/02 ,  C07D265/34 ,  C07D493/10 ,  G01N33/00

CPC分类号： C07D265/38 ,  C07D311/82 ,  C07D493/10 ,  G01N21/77 ,  G01N2021/7786 ,  Y10T436/16 ,  Y10T436/182

摘要： It is an object of the present invention to provide: a novel thiol-detecting reagent, which can be used in vivo and which solves the problem regarding the generation of background fluorescence due to hydrolysis; and a method for detecting thiol using the aforementioned reagent. The present invention provides a compound represented by the following formula (1): wherein each R2 independently represents an alkyl group containing 1 to 6 carbon atoms, a halogen atom, or a hydrogen atom.

摘要翻译： 本发明的目的是提供一种可用于体内的新型巯基检测试剂，其解决了水解产生背景荧光的问题; 和使用上述试剂检测硫醇的方法。 本发明提供由下式（1）表示的化合物：其中各R 2独立地表示含有1〜6个碳原子的烷基，卤素原子或氢原子。

公开(公告)号：US08709721B2

公开(公告)日：2014-04-29

申请号：US13499826

申请日：2010-10-01

申请人： Hiroshi Abe ,  Yoshihiro Ito ,  Aya Shibata ,  Mika Ito

发明人： Hiroshi Abe ,  Yoshihiro Ito ,  Aya Shibata ,  Mika Ito

IPC分类号： C12Q1/68 ,  C07D409/00 ,  C07H19/04

CPC分类号： G01N33/582 ,  C07D311/16 ,  C07D405/12 ,  C07H21/02 ,  C12Q1/6816 ,  C12Q1/6818 ,  G01N33/533 ,  Y02P20/55 ,  Y10T436/143333 ,  C12Q2523/107 ,  C12Q2563/107 ,  C12Q2565/101

摘要： Provided are a fluorescent on/off switchable compound for a gene analysis, which is highly stable and highly sensitive, and enables amplification and observation of a trace gene signal, and a labeling reagent for detection of a bio-related material, which uses the fluorescent on/off switchable compound. A compound represented by the following formula (I′): wherein X is a hydrogen atom or a carboxylic acid-protecting group, Y is a 2,4-dinitrobenzenesulfonyl group, a 2-cyano-4-nitrobenzenesulfonyl group or a 5-nitropyridin-2-ylsulfonyl group, R1, R2, R3 and R4 are each independently a hydrogen atom, a halogen atom, a hydroxyl group, an alkyl group having a carbon number of 1 to 6, an alkoxy group having a carbon number of 1 to 6, an aryl group having a carbon number of 6 to 10, or a cyano group.

摘要翻译： 提供了用于基因分析的荧光开/关可切换化合物，其高度稳定和高度灵敏，并且能够扩增和观察微量基因信号，以及用于检测生物相关材料的标记试剂，其使用荧光 开/关切换组合。 由下式（I'）表示的化合物：其中X是氢原子或羧酸保护基，Y是2,4-二硝基苯磺酰基，2-氰基-4-硝基苯磺酰基或5-硝基吡啶 -2-基磺酰基，R 1，R 2，R 3和R 4各自独立地为氢原子，卤素原子，羟基，碳数为1至6的烷基，碳数为1至 6，碳数为6〜10的芳基或氰基。

公开(公告)号：US20120196377A1

公开(公告)日：2012-08-02

申请号：US13499826

申请日：2010-10-01

申请人： Hiroshi Abe ,  Yoshihiro Ito ,  Aya Shibata ,  Mika Ito

发明人： Hiroshi Abe ,  Yoshihiro Ito ,  Aya Shibata ,  Mika Ito

IPC分类号： G01N21/64 ,  C07D311/16

CPC分类号： G01N33/582 ,  C07D311/16 ,  C07D405/12 ,  C07H21/02 ,  C12Q1/6816 ,  C12Q1/6818 ,  G01N33/533 ,  Y02P20/55 ,  Y10T436/143333 ,  C12Q2523/107 ,  C12Q2563/107 ,  C12Q2565/101

摘要： Provided are a fluorescent on/off switchable compound for a gene analysis, which is highly stable and highly sensitive, and enables amplification and observation of a trace gene signal, and a labeling reagent for detection of a bio-related material, which uses the fluorescent on/off switchable compound. A compound represented by the following formula (I′): wherein X is a hydrogen atom or a carboxylic acid-protecting group, Y is a 2,4-dinitrobenzenesulfonyl group, a 2-cyano-4-nitrobenzenesulfonyl group or a 5-nitropyridin-2-ylsulfonyl group, R1, R2, R3 and R4 are each independently a hydrogen atom, a halogen atom, a hydroxyl group, an alkyl group having a carbon number of 1 to 6, an alkoxy group having a carbon number of 1 to 6, an aryl group having a carbon number of 6 to 10, or a cyano group.

摘要翻译： 提供了用于基因分析的荧光开/关可切换化合物，其高度稳定和高度灵敏，并且能够扩增和观察微量基因信号，以及用于检测生物相关材料的标记试剂，其使用荧光 开/关切换组合。 由下式（I'）表示的化合物：其中X是氢原子或羧酸保护基，Y是2,4-二硝基苯磺酰基，2-氰基-4-硝基苯磺酰基或5-硝基吡啶 -2-基磺酰基，R 1，R 2，R 3和R 4各自独立地为氢原子，卤素原子，羟基，碳数为1至6的烷基，碳数为1至 6，碳数为6〜10的芳基或氰基。

公开(公告)号：US08187825B2

公开(公告)日：2012-05-29

申请号：US12919299

申请日：2009-01-30

申请人： Hiroshi Abe ,  Yoshihiro Ito ,  Aya Shibata ,  Mika Ito

发明人： Hiroshi Abe ,  Yoshihiro Ito ,  Aya Shibata ,  Mika Ito

IPC分类号： G01N33/574 ,  C12Q1/02 ,  A01N55/02 ,  A01N43/16 ,  A01N31/14

CPC分类号： C07D265/38 ,  C07D311/82 ,  C07D493/10 ,  G01N21/77 ,  G01N2021/7786 ,  Y10T436/16 ,  Y10T436/182

摘要： It is an object of the present invention to provide: a novel thiol-detecting reagent, which can be used in vivo and which solves the problem regarding the generation of background fluorescence due to hydrolysis; and a method for detecting thiol using the aforementioned reagent. The present invention provides a compound represented by the following formula (1): wherein each R2 independently represents an alkyl group containing 1 to 6 carbon atoms, a halogen atom, or a hydrogen atom.

摘要翻译： 本发明的目的是提供一种可用于体内的新型巯基检测试剂，其解决了水解产生背景荧光的问题; 和使用上述试剂检测硫醇的方法。 本发明提供由下式（1）表示的化合物：其中各R 2独立地表示含有1〜6个碳原子的烷基，卤素原子或氢原子。

公开(公告)号：US20090227691A1

公开(公告)日：2009-09-10

申请号：US12266061

申请日：2008-11-06

申请人： Hiroshi ABE ,  Yoshihiro ITO ,  Aya SHIBATA ,  Hiroaki KODAMA

发明人： Hiroshi ABE ,  Yoshihiro ITO ,  Aya SHIBATA ,  Hiroaki KODAMA

IPC分类号： C12Q1/68 ,  C07C69/76 ,  C09B29/00 ,  A61K47/30

CPC分类号： C12Q1/6818 ,  A61K47/549 ,  A61K47/558 ,  C07C237/04 ,  C07C245/08 ,  C07D211/62 ,  C12Q2565/30

摘要： It is an object of the present invention to provide a method for highly selectively releasing a molecule of interest such as an agent at a desired site while suppressing the influence of catabolic enzymes existing in vivo. The present invention provides a method for releasing a molecule of interest, which comprises steps of: hybridizing each of an “electron donor-first nucleic acid probe” molecule formed by binding an electron donor structure to a first nucleic acid probe having a nucleotide sequence complementary to a portion of a target nucleic acid sequence and a “molecule of interest-electron acceptor-second nucleic acid probe” molecule formed by binding an electron acceptor structure having a molecule of interest and an azide group to a second nucleic acid probe that has a nucleotide sequence complementary to said target nucleic acid sequence and differing from that of said first nucleic acid probe, to said target nucleic acid sequence; and allowing said “electron donor-first nucleic acid probe” molecule to act on said “molecule of interest-electron acceptor-second nucleic acid probe” molecule, so as to release said molecule of interest.

摘要翻译： 本发明的目的是提供一种用于在抑制现有的分解代谢酶的影响的同时，在期望的位置高度选择性释放感兴趣的分子例如试剂的方法。 本发明提供一种释放感兴趣分子的方法，其包括以下步骤：将通过结合电子供体结构形成的“电子给体 - 第一核酸探针”分子与具有核苷酸序列互补的第一核酸探针杂交 通过将具有目标分子和叠氮基的电子受体结构与靶核酸序列的一部分和通过将具有目标分子和叠氮基的电子受体结构结合到第二核酸探针而形成的“核酸分子 - 电子受体 - 第二核酸探针” 与所述靶核酸序列互补且与所述第一核酸探针不同的核苷酸序列与所述靶核酸序列互补; 并且允许所述“电子供体 - 第一核酸探针”分子作用于所述“感兴趣的分子 - 电子受体 - 第二核酸探针”分子上，以释放所述感兴趣的分子。