公开(公告)号：US5631169A

公开(公告)日：1997-05-20

申请号：US183238

申请日：1994-01-19

申请人： Joseph R. Lakowicz ,  Badri P. Maliwal ,  Richard Thompson ,  Alvydas Ozinskas

发明人： Joseph R. Lakowicz ,  Badri P. Maliwal ,  Richard Thompson ,  Alvydas Ozinskas

IPC分类号： G01N21/64 ,  G01N33/542 ,  G01N33/543 ,  G01N33/58 ,  G01N33/533

CPC分类号： G01N33/582 ,  G01N21/6408 ,  G01N21/6428 ,  G01N33/542 ,  G01N2021/6441 ,  Y10S436/80

摘要： A fluorometric luminescence immunoassay method includes forming a sample by exposing a first immune reaction reactant to a second immune reaction reactant capable of reacting with the first reactant, one of the first and second immune reaction reactants being labelled with a photoluminescent energy transfer donor and the other being labelled with a photoluminescent energy transfer acceptor complementary to the photoluminescent donor. At least the photoluminescent donor has the property of photoluminescence, and the photoluminescent donor and acceptor are chosen so that when the first immune reaction reactant reacts with the second immune reaction reactant, the donor and the acceptor are capable of interacting to produce a detectable luminescence lifetime change. The sample is excited with radiation, and the resulting emission is detected. The apparent luminescent lifetime is then calculated to determine the presence of a reaction product of the first and second immune reaction reactants.

摘要翻译： 荧光发光免疫测定方法包括通过将第一免疫反应反应物暴露于能够与第一反应物反应的第二免疫反应反应物形成样品，第一和第二免疫反应物之一用光致发光能量转移供体标记，另一个 用与光致发光供体互补的光致发光能量转移受体标记。 至少光致发光供体具有光致发光的性质，并且选择光致发光供体和受体，使得当第一免疫反应反应物与第二免疫反应物反应时，供体和受体能够相互作用以产生可检测的发光寿命 更改。 用辐射激发样品，并检测所得到的发射。 然后计算表观发光寿命以确定第一和第二免疫反应物的反应产物的存在。

公开(公告)号：US5246867A

公开(公告)日：1993-09-21

申请号：US822382

申请日：1992-01-17

申请人： Joseph R. Lakowicz ,  Badri P. Maliwal ,  Peter A. Koen

发明人： Joseph R. Lakowicz ,  Badri P. Maliwal ,  Peter A. Koen

IPC分类号： A61B5/145 ,  A61B5/1455 ,  A61B5/1459 ,  G01N21/64 ,  G01N21/76 ,  G01N21/78 ,  G01N33/532 ,  G01N33/542 ,  G01N33/58 ,  G01N33/66

CPC分类号： G01N21/6408 ,  G01N33/582 ,  G01N33/66 ,  Y10T436/143333 ,  Y10T436/144444

摘要： A method for measuring the concentration of a saccharide, conjugated saccharide or polysaccharide of interest using luminescent lifetimes and energy transfer in which an energy transfer donor-acceptor pair is added to a sample to be analyzed, the donor of the donor-acceptor pair being photoluminescent. The acceptor is bound to a carrier, while the donor and any saccharide, conjugated saccharide or polysaccharide of interest present in the sample compete for binding sites on the carrier. The sample is irradiated and the resultant emission detected. Energy transfer occurs between the donors and the acceptors, which produces a detectable lifetime change of the fIuorescence of the donor. The lifetime change is reduced or even eliminated by the competitive binding of a saccharide, conjugated saccharide or polysaccharide of interest to the donor. By measuring the apparent luminescent lifetime, the amount of a saccharide, conjugated saccharide or polysaccharide of interest in the sample can be determined.

公开(公告)号：US06284544B1

公开(公告)日：2001-09-04

申请号：US09071351

申请日：1998-04-30

申请人： Richard B. Thompson ,  Daniel Elbaum ,  Vincent L. Feliccia ,  David Christianson ,  Marcia W. Patchan ,  Zhengfang Ge ,  Badri P. Maliwal

发明人： Richard B. Thompson ,  Daniel Elbaum ,  Vincent L. Feliccia ,  David Christianson ,  Marcia W. Patchan ,  Zhengfang Ge ,  Badri P. Maliwal

IPC分类号： G01N3320

CPC分类号： G01N33/542 ,  G01N21/6445 ,  G01N33/20 ,  G01N33/582 ,  G01N2333/988

摘要： Homogeneous fluorescence polarization (anisotropy) assays for detecting and quantifying metal ions in solution, based the metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule, or the binding of a metal ion to a fluorescent labeled macromolecule. The metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule (metallo-macromolecule) effects a measurable change in anisotropy as will the binding of metal ions to a fluorescent labeled macromolecule. Binding of the fluorescent ligand to the unlabeled macromolecule is metal dependent with the change in anisotropy being proportional to the concentration of bound metal ions. No binding of the fluorescent ligand to the macromolecule occurs in the absence of metal ions. Conversely, if the fluorescent label is first conjugated to a metallo-macromolecule and the metallo-macromolecule is subsequently stripped of its metal ion, it may then be used to transduce the binding of metal ions. Transduction is provided wherein the covalently bound fluorescent label exhibits changes in anisotropy proportional to the concentration of bound metal ions. In all methods, the change in anisotropy may be simply related to the metal ion concentration of the test solution.

摘要翻译： 基于荧光配体与未标记的大分子的金属依赖性结合，或金属离子与荧光标记的大分子的结合，用于检测和定量溶液中的金属离子的均匀荧光偏振（各向异性）测定。 荧光配体与未标记的大分子（金属 - 大分子）的金属依赖性结合对金属离子与荧光标记的大分子的结合将产生各向异性的可测量的变化。 荧光配体与未标记的大分子的结合是金属依赖性的，各向异性的变化与结合的金属离子的浓度成比例。 在不存在金属离子的情况下，不发生荧光配体与大分子的结合。 相反，如果荧光标记首先与金属 - 大分子缀合，并且金属 - 大分子随后被其金属离子剥离，则其可用于转导金属离子的结合。 提供转导，其中共价结合的荧光标记表现出与结合的金属离子浓度成比例的各向异性变化。 在各种方法中，各向异性的变化可能与测试溶液的金属离子浓度有关。

公开(公告)号：US07842491B2

公开(公告)日：2010-11-30

申请号：US10982956

申请日：2004-11-08

申请人： Richard B. Thompson ,  Daniel Elbaum ,  Vincent L. Feliccia ,  David Christianson ,  Marcia W. Patchan ,  Zhengfang Ge ,  Badri P. Maliwal

发明人： Richard B. Thompson ,  Daniel Elbaum ,  Vincent L. Feliccia ,  David Christianson ,  Marcia W. Patchan ,  Zhengfang Ge ,  Badri P. Maliwal

IPC分类号： C12Q1/34 ,  C12Q1/00 ,  G01N21/00 ,  G01N33/20

CPC分类号： G01N33/542 ,  C12N9/88 ,  G01N21/6445 ,  Y10S435/975

摘要： The invention relates to compositions and kits for homogeneous fluorescence polarization (anisotropy) assays for detecting and quantifying metal ions in solution. Metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule effects a measurable change in anisotropy as will the binding of metal ions to a fluorescent labeled macromolecule. Binding of the fluorescent ligand to the unlabeled macromolecule is metal dependent with the change in anisotropy being proportional to the concentration of bound metal ions. Conversely, if the fluorescent label is first conjugated to a macromolecule and the macromolecule is subsequently stripped of metal ion, it may then be used to signal binding of metal ions. The covalently bound fluorescent label exhibits changes in anisotropy proportional to the concentration of bound metal ions. Kits comprise a fluorescent molecule and a macromolecule.

摘要翻译： 本发明涉及用于检测和定量溶液中的金属离子的均匀荧光偏振（各向异性）测定的组合物和试剂盒。 荧光配体与未标记的大分子的金属依赖性结合影响各向异性的可测量的变化，以及金属离子与荧光标记的大分子的结合。 荧光配体与未标记的大分子的结合是金属依赖性的，各向异性的变化与结合的金属离子的浓度成比例。 相反，如果荧光标记首先与大分子缀合并且随后大分子被金属离子剥离，则可以用它来信号结合金属离子。 共价结合的荧光标记表现出与结合金属离子浓度成比例的各向异性变化。 试剂盒包含荧光分子和大分子。

公开(公告)号：US06197258B1

公开(公告)日：2001-03-06

申请号：US09273303

申请日：1999-03-19

申请人： Richard B. Thompson ,  Vincent L. Feliccia ,  Badri P. Maliwal ,  Carol A. Fierke

发明人： Richard B. Thompson ,  Vincent L. Feliccia ,  Badri P. Maliwal ,  Carol A. Fierke

IPC分类号： G01N3320

CPC分类号： G01N33/582 ,  G01N33/20 ,  G01N33/542 ,  G01N2333/988

摘要： The invention described in detail herein relates to the detection, determination, and quantitation of certain ions and small molecules in solution. The invention specifically relates to improvements in the area of photoluminescent sensors for use in a detection scheme involving the alteration of a photoluminescent label or moiety attached to or associated with an analyte binding macromolecule. One may use the changes in photoluminescence lifetime, changes in ratios of photoluminescence intensity or changes in photoluminescence polarization (anisotropy) to determine the analyte. The photoluminescence change measured correlates to the concentration of the ion or molecule in solution.

摘要翻译： 本文详细描述的本发明涉及溶液中某些离子和小分子的检测，测定和定量。 本发明具体涉及用于检测方案中的光致发光传感器的改进，所述检测方案涉及改变附着于或与分析物结合大分子结合的光致发光标记或部分。 可以使用光致发光寿命的变化，光致发光强度比例的变化或光致发光偏振的变化（各向异性）来确定分析物。 测量的光致变化与溶液中离子或分子的浓度相关。