公开(公告)号：US20050239180A1

公开(公告)日：2005-10-27

申请号：US10514031

申请日：2003-05-06

申请人： Hermann Sahm ,  Gerald Hahn ,  Stephanie Bringer-Meyer ,  Bjorn Kaup ,  Claudia Hemmerling ,  Martin Walter ,  Dieter Wullbrandt

发明人： Hermann Sahm ,  Gerald Hahn ,  Stephanie Bringer-Meyer ,  Bjorn Kaup ,  Claudia Hemmerling ,  Martin Walter ,  Dieter Wullbrandt

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N9/04 ,  C12P7/18 ,  C07H21/04 ,  C12N9/02 ,  C12N15/74

CPC分类号： C12N9/0006 ,  C12P7/18

摘要： The invention relates to a nucleotide sequence coding for the mannitol 2-dehydrogenase and a method for producing D-mannitol. Previously known biocatalytic methods for producing D-mannitol yield only small production rates due to the small number of specific activities of mannitol 2-dehydrogenases used for transforming D-fructose into D-mannitol. D-mannitol production can be improved by supplying a nucleotide sequence which codes for a mannitol 2-dehydrogenase having a higher specific activity. Mannitol production can be increased in a particular manner by creating a regeneration system for reduction equivalents by introducing and/or strengthening a formiate dehydrogenase in a microorganism.

摘要翻译： 本发明涉及编码甘露醇2-脱氢酶的核苷酸序列和D-甘露糖醇的制造方法。 由于用于将D-果糖转化为D-甘露糖醇的甘露醇2-脱氢酶的比活性较少，以前已知的用于生产D-甘露糖的生物催化方法产生的生产速率较小。 通过提供编码具有较高比活性的甘露糖醇2-脱氢酶的核苷酸序列可以改善D-甘露糖醇的产生。 通过在微生物中引入和/或强化甲酸脱氢酶，通过产生用于还原当量的再生系统，可以以特定方式增加甘露糖醇生产。