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    Process for reacting substrates with biocatalysts in a 2-phase liquid system
    5.
    发明授权
    Process for reacting substrates with biocatalysts in a 2-phase liquid system 失效
    使底物与生物催化剂在2相液体体系中反应的方法

    公开(公告)号：US4806477A

    公开(公告)日：1989-02-21

    申请号：US055541

    申请日：1987-05-29

    申请人： Reinhardt Rothert ,  Dieter Wullbrandt

    发明人： Reinhardt Rothert ,  Dieter Wullbrandt

    IPC分类号： C12M1/02 ,  C12M1/40 ,  C12N11/02 ,  C12P41/00

    CPC分类号： C12M41/22 ,  C12M25/20 ,  C12M27/02 ,  C12M29/04 ,  C12M29/18 ,  C12M33/22

    摘要： A process for reacting substrates with biocatalysts in a two-phase liquid system disposed in a columnar vessel having a top portion with an exit opening and a first filter disposed therein, a bottom portion with an entrance opening and a second filter disposed therein, a feed line connecting the exit and entrance openings, and a pump disposed in the feed line. The process comprises the steps of: stirring the two-phase liquid in the columnar vessel to accelerate sedimentation and minimize accumulation of the biocatalysts in the top portion of the vessel; selectively controlling the temperature of the two-phase liquid within the vessel; drawing, with the pump, a portion of the two-phase liquid through the exit opening and first filter; and passing the drawn-off portion of the two-phase liquid through the feed line and through the entrance opening and second filter into the vessel to enhance miscibility of the individual phases of the two-phase liquid with the vessel.

    摘要翻译： 一种将底物与生物催化剂反应在两相液体系统中的方法，所述两相液体系统设置在具有顶部的柱状容器中，所述顶部具有出口和设置在其中的第一过滤器，底部具有入口和设置在其中的第二过滤器， 连接出口和入口的管线，以及设置在进料管线中的泵。 该方法包括以下步骤：搅拌柱状容器中的两相液体以加速沉淀并最小化生物催化剂在容器顶部的积聚; 选择性地控制容器内的两相液体的温度; 通过泵抽出一部分两相液体通过出口和第一过滤器; 并将两相液体的引出部分通过进料管线并通过入口和第二过滤器进入容器，以增强两相液体的各相与容器的混溶性。

    Litter for cats and small animals
    6.
    发明授权
    Litter for cats and small animals 失效
    猫和小动物的垃圾

    公开(公告)号：US06860233B2

    公开(公告)日：2005-03-01

    申请号：US10451462

    申请日：2001-12-21

    申请人： Christoph Buttersack ,  Dieter Wullbrandt

    发明人： Christoph Buttersack ,  Dieter Wullbrandt

    IPC分类号： A01K1/015 ,  A01K29/00

    CPC分类号： A01K1/0154 ,  A01K1/0155

    摘要： Litter for cats and small animals often consists of a mixture from dried material of plant origin and an adjuvant that promotes agglomeration when the mixture is exposed to moisture. The invention relates to a novel litter that is characterized in that the dried material of plant origin is present in a particle size range in which the diameters of 80% of the particles, based on the mass, range from more than 0 μm to less than 1000 μm. The mass sum distribution of the particle size distribution, at least in the range between 50 μm and 1000 μm, is larger than an integrated statistical Gaussian distribution at which the Gaussian distribution is a normal Gaussian distribution with an average value of 100 μm and a standard deviation of 800 μm and at which the particle size distribution deviates by more than 10% from this statistic Gaussian distribution.

    摘要翻译： 猫和小动物的垃圾通常由植物来源的干燥物质和当混合物暴露于水分时促进团聚的佐剂组成。 本发明涉及一种新型垃圾，其特征在于，植物来源的干燥材料以粒度范围存在，其中基于质量的80％的颗粒的直径范围从大于0um到小于 1000个妈妈 颗粒尺寸分布的质量和分布至少在50μm到1000μm之间的范围内，大于高斯分布是平均值为100μm的标准高斯分布的统一高斯分布，标准 800微米的偏差，其粒度分布偏离该统计高斯分布超过10％。

    Sophoroselipids, method for their production and use
    7.
    发明授权
    Sophoroselipids, method for their production and use 失效
    苦参皂甙，其生产和使用方法

    公开(公告)号：US06433152B1

    公开(公告)日：2002-08-13

    申请号：US09530769

    申请日：2000-07-03

    申请人： Siegmund Lang ,  Andreas Brakemeier ,  Dieter Wullbrandt ,  Andreas Seiffert-Störicko

    发明人： Siegmund Lang ,  Andreas Brakemeier ,  Dieter Wullbrandt ,  Andreas Seiffert-Störicko

    IPC分类号： C07G300

    CPC分类号： C11D1/667 ,  A01N43/16 ,  A61K8/602 ,  A61K2800/85 ,  A61Q19/00 ,  C07H15/04 ,  C12P19/46

    摘要： The invention relates to sophoroselipids of formula (I) wherein R4 represents H, —CH2CH3, —CH2CH2CH3, n is an integer number from 2 to 27, R1 and R2 represent, independently from each other, H or a group of formula (II), and R3 represents H or —OH. The invention also relates to a method for producing sophoroselipids of formula (I) wherein R1, R2, R3 and n have the aforementioned meanings and R4 represents —CH3, —CH2CH3 or —CH2CH2CH3. According to this method, a yeast capable of secreting a sophoroselipid in the form of a lactone in the culture excess, is fermented in a culture medium containing a glycerin. a succinate, a mono-, di- and/or tri-saccharide and a lipid precursor, said sophoroslipid being then isolated from the culture solution. The inventive method is characterised in that the lipid precursor contains one or more 3-alkanols, 4-alkanols or an alkanone with a chain length from 6 to 30 carbon atoms or mixtures of said alkanoistalkanone. Furthermore, according to a variant of the aforementioned method for producing sophoroselipids of formula (I), wherein R1, R2, R3 and n have the meanings aforementioned and R4 represents —CH3, —CH2CH3 or —CH2CH2CH3, the culture medium is maintained during fermentation under a reduced oxygen concentration. The new sophoroselipids may be used, e.g., as surfactants, cosmetics, disinfecting agents or pharmaceutical products.

    摘要翻译： 本发明涉及式（I）的槐糖脂，其中R 4表示H，-CH 2 CH 3，-CH 2 CH 2 CH 3，n是2至27的整数，R 1和R 2彼此独立地表示H或式（II）的基团， ，R 3表示H或-OH。 本发明还涉及制备式（I）的槐糖脂的方法，其中R 1，R 2，R 3和n具有上述含义，R 4表示-CH 3，-CH 2 CH 3或-CH 2 CH 2 CH 3。 根据该方法，将能够分泌培养过量的内酯形式的槐糖脂的酵母在含有甘油的培养基中发酵。 琥珀酸盐，单糖，二糖和/或三糖和脂质前体，然后从培养溶液中分离出所述槐糖脂。 本发明的方法的特征在于脂质前体含有一个或多个3-链烷醇，4-链烷醇或链长为6-30个碳原子的烷酮或所述烷酰基烷酮的混合物。 此外，根据上述制备式（I）的槐糖脂的方法的变体，其中R1，R2，R3和n具有上述含义，R4代表-CH3，-CH2CH3或-CH2CH2CH3，培养基在发酵期间保持 在氧浓度降低的情况下。 可以使用新的槐糖脂，例如作为表面活性剂，化妆品，消毒剂或药物产品。

    Pseudomonas aeruginosa and its use in a process for the biotechnological preparation of L-rhamnose
    8.
    发明授权
    Pseudomonas aeruginosa and its use in a process for the biotechnological preparation of L-rhamnose 失效
    铜绿假单胞菌及其在L-鼠李糖生物技术制备过程中的应用

    公开(公告)号：US5501966A

    公开(公告)日：1996-03-26

    申请号：US375230

    申请日：1995-01-19

    申请人： Carlo Giani ,  Dieter Wullbrandt ,  Reinhardt Rothert ,  Johannes Meiwes

    发明人： Carlo Giani ,  Dieter Wullbrandt ,  Reinhardt Rothert ,  Johannes Meiwes

    IPC分类号： C12N1/20 ,  C12P19/02 ,  C12P19/12 ,  C12P19/44 ,  C12R1/385

    CPC分类号： C12R1/385 ,  C12P19/02 ,  C12P19/12 ,  C12P19/44

    摘要： Pseudomonas aeruginosa and its use in a process for the biotechnological production of L-rhamnoseBacterial strains are capable of the formation of rhamnolipids, which they generate in the culture solution. If bacteria of the type Pseudomonas aeruginosa are employed for the fermentation, these microorganisms synthesize rhamnolipids in a concentration of 70-120 g/l of culture solution. The L-rhamnose can be recovered directly from the culture solution by hydrolysis of the rhamnolipids, i.e. without a complicated separation of the cell material and without isolation of the rhamnolipids before hydrolysis.

    摘要翻译： 铜绿假单胞菌及其在L-鼠李糖生物技术生产过程中的应用细菌菌株能够在培养液中形成鼠李糖脂。 如果将铜绿假单胞菌的细菌用于发酵，则这些微生物合成浓度为70-120g / l培养液的鼠李糖脂。 L-鼠李糖可以通过鼠李糖脂的水解从培养液中直接回收，即没有细胞材料的复杂分离，而在水解之前不分离鼠李糖脂。