公开(公告)号：US20050239180A1

公开(公告)日：2005-10-27

申请号：US10514031

申请日：2003-05-06

申请人： Hermann Sahm ,  Gerald Hahn ,  Stephanie Bringer-Meyer ,  Bjorn Kaup ,  Claudia Hemmerling ,  Martin Walter ,  Dieter Wullbrandt

发明人： Hermann Sahm ,  Gerald Hahn ,  Stephanie Bringer-Meyer ,  Bjorn Kaup ,  Claudia Hemmerling ,  Martin Walter ,  Dieter Wullbrandt

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N9/04 ,  C12P7/18 ,  C07H21/04 ,  C12N9/02 ,  C12N15/74

CPC分类号： C12N9/0006 ,  C12P7/18

摘要： The invention relates to a nucleotide sequence coding for the mannitol 2-dehydrogenase and a method for producing D-mannitol. Previously known biocatalytic methods for producing D-mannitol yield only small production rates due to the small number of specific activities of mannitol 2-dehydrogenases used for transforming D-fructose into D-mannitol. D-mannitol production can be improved by supplying a nucleotide sequence which codes for a mannitol 2-dehydrogenase having a higher specific activity. Mannitol production can be increased in a particular manner by creating a regeneration system for reduction equivalents by introducing and/or strengthening a formiate dehydrogenase in a microorganism.

摘要翻译： 本发明涉及编码甘露醇2-脱氢酶的核苷酸序列和D-甘露糖醇的制造方法。 由于用于将D-果糖转化为D-甘露糖醇的甘露醇2-脱氢酶的比活性较少，以前已知的用于生产D-甘露糖的生物催化方法产生的生产速率较小。 通过提供编码具有较高比活性的甘露糖醇2-脱氢酶的核苷酸序列可以改善D-甘露糖醇的产生。 通过在微生物中引入和/或强化甲酸脱氢酶，通过产生用于还原当量的再生系统，可以以特定方式增加甘露糖醇生产。

公开(公告)号：US5017485A

公开(公告)日：1991-05-21

申请号：US448334

申请日：1989-12-11

申请人： Stephanie Bringer-Meyer ,  Hermann Sahm

发明人： Stephanie Bringer-Meyer ,  Hermann Sahm

IPC分类号： C12P7/18 ,  C12P7/58

CPC分类号： C12P7/18 ,  C12P7/58 ,  Y10S435/822

摘要： A process is disclosed for obtaining sorbitol and gluconic acid, or gluconate, by fermentation in aqueous glucose/fructose mixtures. Zymomonas mobilis cells are used which have been permeabilized by the freeze technique and which have preferably been obtained by freezing cell centrifugates of pH 6 to 7 at about -20.degree. C., and thawing at room temperature. Cell concentrations of 20 to 60 g of cell dry matter of permeabilized cells/l are preferably used for the fermentation. By using cells which have been permeabilized by freezing and thawing, a considerably higher conversion rate of glucose/fructose into sorbitol and gluconic acid, or gluconate, is achieved.

摘要翻译： 公开了通过在水性葡萄糖/果糖混合物中发酵获得山梨糖醇和葡萄糖酸或葡萄糖酸盐的方法。 使用通过冷冻技术透化的运动发酵单胞菌细胞，其优选通过在约-20℃下冷冻pH6-7的细胞离心液并在室温下解冻获得。 细胞浓度为20〜60g的透化细胞/ l细胞干物质优选用于发酵。 通过使用通过冷冻和解冻而透化的细胞，实现了相当高的葡萄糖/果糖转化为山梨醇和葡糖酸或葡萄糖酸的转化率。

公开(公告)号：US5804423A

公开(公告)日：1998-09-08

申请号：US594808

申请日：1996-01-31

申请人： Ralf Klasen ,  Stephanie Bringer-Meyer ,  Hermann Sahm ,  Cornelies Petrus Hollenberg

发明人： Ralf Klasen ,  Stephanie Bringer-Meyer ,  Hermann Sahm ,  Cornelies Petrus Hollenberg

IPC分类号： C12N1/21 ,  C12N15/53 ,  C12P7/58 ,  C12P7/60 ,  C07H21/04 ,  C12N1/20 ,  C12N9/02

CPC分类号： C12P7/58

摘要： A method is disclosed for preparing 5-ketogluconate, which comprises the steps of: (a) genetically modifying a microorganism capable of microbiologically producing 5-ketogluconate to increase gluconate NADP.sup.+ -5-oxidoreductase gene expression of said microorganism; (b) culturing said microorganism in a medium to produce 5-ketogluconate; and (c) recovering 5-ketogluconate from said medium.

摘要翻译： 公开了一种制备5-酮葡萄糖酸盐的方法，其包括以下步骤：（a）遗传修饰能够微生物产生5-酮葡萄糖酸盐的微生物，以增加所述微生物的葡萄糖酸化NADP + 5-氧化还原酶基因表达; （b）在培养基中培养所述微生物以产生5-酮葡萄糖酸盐; 和（c）从所述培养基中回收5-酮葡萄糖酸盐。

公开(公告)号：US08119374B2

公开(公告)日：2012-02-21

申请号：US12136190

申请日：2008-06-10

申请人： Petra Peters-Wendisch ,  Roman Netzer ,  Lothar Eggeling ,  Hermann Sahm

发明人： Petra Peters-Wendisch ,  Roman Netzer ,  Lothar Eggeling ,  Hermann Sahm

IPC分类号： C12P13/06 ,  C12P21/06 ,  C12N9/88

CPC分类号： C12N9/88 ,  C12P13/06

摘要： This invention relates to the nucleotide sequence of coryneform bacteria coding for proteins which are involved in L-serine metabolism with reduced and switched off L-serine dehydratase activity. The invention also relates to microorganisms used in methods for producing L-serine.

摘要翻译： 本发明涉及编码与L-丝氨酸脱水酶活性降低和关闭的L-丝氨酸代谢相关的蛋白质的棒状细菌的核苷酸序列。 本发明还涉及用于生产L-丝氨酸的方法中使用的微生物。

公开(公告)号：US20100261233A1

公开(公告)日：2010-10-14

申请号：US12249092

申请日：2008-10-10

申请人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

发明人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

IPC分类号： C12P13/08

CPC分类号： C12N15/52 ,  C07K14/34 ,  C12P13/08

摘要： L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

摘要翻译： 具有增强的lysE基因（赖氨酸出口载体基因）的L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因）的lys附加基因，lysC基因（天冬氨酸激酶基因），dapB基因 （二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）增强，特别是过表达，以及制备L-赖氨酸的方法。

公开(公告)号：US20080153139A1

公开(公告)日：2008-06-26

申请号：US11663984

申请日：2006-04-06

申请人： Jan Marienhagen ,  Lothar Eggeling ,  Hermann Sahm

发明人： Jan Marienhagen ,  Lothar Eggeling ,  Hermann Sahm

IPC分类号： C12P13/08 ,  C12N1/21

CPC分类号： C12P13/08 ,  C12N9/1096

摘要： The invention relates to a method for producing L-valine and to a suitable microorganism. The inventive method is characterized by preferably enhancing the transaminase C activity of a coryneform bacterium, especially Corynebacteriuim glutamicum. The organisms so modified have a yield in L-valine which is 35.8% higher than that of non-modified organisms.

摘要翻译： 本发明涉及L-缬氨酸和合适微生物的生产方法。 本发明的方法的特征在于优选增强棒状细菌，特别是谷氨酸棒杆菌（Corynebacteriuim glutamicum）的转氨酶C活性。 如此修饰的生物体在L-缬氨酸中的产率高于非改性生物体的35.8％。

公开(公告)号：US20070054380A1

公开(公告)日：2007-03-08

申请号：US11425091

申请日：2006-06-19

申请人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

发明人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

IPC分类号： C12P13/08 ,  C07H21/04 ,  C12N15/74 ,  C12N1/21

CPC分类号： C12N15/52 ,  C07K14/34 ,  C12P13/08

摘要： The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

摘要翻译： 本发明涉及具有增强的lysE基因（赖氨酸出口载体基因）的棒状杆菌L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因） ，dapB基因（二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）被增强，特别是过度表达，并且涉及制备L- 赖氨酸。

公开(公告)号：US07094584B2

公开(公告)日：2006-08-22

申请号：US10804120

申请日：2004-03-19

申请人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Möckel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

发明人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Möckel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

IPC分类号： C12P13/08

CPC分类号： C12N15/52 ,  C07K14/34 ,  C12P13/08

摘要： The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

摘要翻译： 本发明涉及具有增强的lysE基因（赖氨酸出口载体基因）的棒状杆菌L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因） ，dapB基因（二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）被增强，特别是过度表达，并且涉及制备L- 赖氨酸。

公开(公告)号：US06913909B2

公开(公告)日：2005-07-05

申请号：US09963521

申请日：2001-09-27

申请人： Petra Ziegler ,  Lothar Eggeling ,  Hermann Sahm ,  Georg Thierbach

发明人： Petra Ziegler ,  Lothar Eggeling ,  Hermann Sahm ,  Georg Thierbach

IPC分类号： C12N15/09 ,  C07K14/34 ,  C12N1/21 ,  C12N15/01 ,  C12N15/31 ,  C12N15/77 ,  C12P13/08 ,  C12R1/15 ,  C12N1/20 ,  C12N15/00 ,  C12P21/06 ,  C12Q1/48

CPC分类号： C07K14/34 ,  C12P13/08

摘要： The invention relates to preferably recombinant DNA derived from Corynebacterium and replicable in coryneform microorganisms, which contains at least one nucleotide sequence that codes for the thrE gene, and a process for the production of L-threonine, which is characterised in that the following steps are carried out: a) Fermentation of microorganisms in which at least the thrE gene is amplified (overexpressed), optionally in combination with further genes, b) Enrichment of the L-threonine in the medium or in the cells of the microorganisms, and c) Isolation of the L-threonine.

摘要翻译： 本发明涉及优选来源于棒状杆菌的重组DNA，并可在包含编码thrE基因的至少一个核苷酸序列的棒状细菌中复制，以及L-苏氨酸的制备方法，其特征在于以下步骤为 进行：a）至少将thrE基因扩增（过表达）的微生物发酵，任选与其它基因组合，b）在培养基中或微生物细胞中富集L-苏氨酸，和c） L-苏氨酸的分离。

公开(公告)号：US06562607B2

公开(公告)日：2003-05-13

申请号：US09848726

申请日：2001-05-04

申请人： Madhavan Nampoothiri K. ,  Bettina Möckel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm

发明人： Madhavan Nampoothiri K. ,  Bettina Möckel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm

IPC分类号： C12N900

CPC分类号： C12N9/1288 ,  C12P13/14

摘要： This invention relates to a genetically modified coryneform bacterium, the cls gene of which is amplified, and to an isolated polynucleotide, which codes for cardiolipin synthase from coryneform bacteria and to a process for the fermentative production of L-amino acids with amplification of the cls gene in the bacteria and to the use of the polynucleotide as a primer or hybridization probe.

摘要翻译： 本发明涉及基因修饰的棒状细菌，其cls基因被扩增，并且分离的多核苷酸编码来自棒状细菌的心磷脂合成酶，以及用于扩增cls的L-氨基酸的发酵生产方法 基因和使用多核苷酸作为引物或杂交探针。