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公开(公告)号:US5739407A
公开(公告)日:1998-04-14
申请号:US78090
申请日:1993-06-18
申请人: Sven Bergstrom , Olle Hernell , Bo Lonnerdal , Karin Hjalmarsson , Lennart Hanson , Jan Tornell , Mats Stromqvigt
发明人: Sven Bergstrom , Olle Hernell , Bo Lonnerdal , Karin Hjalmarsson , Lennart Hanson , Jan Tornell , Mats Stromqvigt
IPC分类号: A01K67/027 , A23C9/152 , C07K14/47 , C07K14/81 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/00 , C12N15/09 , C12N15/12 , C12N15/85 , C12P21/02 , C12R1/19 , C12R1/865 , C12R1/91 , C12N5/00
CPC分类号: C12N15/8509 , A01K67/0275 , A01K67/0278 , C07K14/4732 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/10 , A01K2227/101 , A01K2227/105 , A01K2267/01 , Y10S426/801
摘要: The present invention relates to a DNA sequence encoding the human milk protein .beta.-casein or an analogue or variant thereof which has either the calcium binding activity of human .beta.-casein, or opioid activity, or angiotensin converting enzyme (ACE) inhibitory activity, or a combination of any two or three of these activities. The DNA sequence may optionally contain one or more intron sequences and permissive RNA splice signals. The DNA sequence is used in the production of recombinant human .beta.-casein, advantageously by means of production in transgenic non-human mammals such as bovine species. In one embodiment, the DNA sequence is inserted into a milk protein gene of a mammal such as a whey acidic protein (WAP) gene. The main use of the recombinant human .beta.-casein is as a constituent of infant formulae. It is contemplated that the recombinant human .beta.-casein provides a substantial improvement of the nutritional and biological value of the formulae in that a closer similarity to human milk is obtained.
摘要翻译: 本发明涉及编码人乳蛋白β-酪蛋白或其类似物或变体的DNA序列,其具有人β-酪蛋白的钙结合活性或阿片样物质活性或血管紧张素转换酶(ACE)抑制活性,或 任何两个或三个这些活动的组合。 DNA序列可以任选地含有一个或多个内含子序列和允许的RNA剪接信号。 DNA序列用于生产重组人β-酪蛋白,有利地通过在转基因非人哺乳动物如牛物种中的生产。 在一个实施方案中,将DNA序列插入哺乳动物如乳清酸性蛋白(WAP)基因的乳蛋白基因中。 重组人β-酪蛋白的主要用途是作为婴儿配方奶粉的成分。 预期重组人β-酪蛋白提供了配方的营养和生物学价值的显着改善,因为获得了更接近于人乳的相似性。