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公开(公告)号：US09365826B2
公开(公告)日：2016-06-14
申请号：US13164461
申请日：2011-06-20
申请人： Nathan Meyer , Brad Swanson , Steve Fiene
发明人： Nathan Meyer , Brad Swanson , Steve Fiene
IPC分类号： C12N5/077
CPC分类号： C12N5/0657 , C12N2500/00 , C12N2501/999
摘要： Methods and composition for maintenance of cardiomyocytes are provided. For example, in certain aspects methods including culturing the cardiomyocytes in a medium essentially free of serum or containing dialyzed serum to maintain long-term purity. In further aspects, methods for modulation of cardiomyocytes may be provided.
摘要翻译： 提供了维持心肌细胞的方法和组成。 例如，在某些方面，包括在基本上不含血清或含有透析血清的培养基中培养心肌细胞以保持长期纯度的方法。 在另外的方面，可以提供用于调节心肌细胞的方法。
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公开(公告)号：US20110312090A1
公开(公告)日：2011-12-22
申请号：US13164461
申请日：2011-06-20
申请人： Nathan Meyer , Brad Swanson , Steve Fiene
发明人： Nathan Meyer , Brad Swanson , Steve Fiene
IPC分类号： C12N5/077
CPC分类号： C12N5/0657 , C12N2500/00 , C12N2501/999
摘要： Methods and composition for maintenance of cardiomyocytes are provided. For example, in certain aspects methods including culturing the cardiomyocytes in a medium essentially free of serum or containing dialyzed serum to maintain long-term purity. In further aspects, methods for modulation of cardiomyocytes may be provided.
摘要翻译： 提供了维持心肌细胞的方法和组成。 例如，在某些方面，包括在基本上不含血清或含有透析血清的培养基中培养心肌细胞以保持长期纯度的方法。 在另外的方面，可以提供用于调节心肌细胞的方法。
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公开(公告)号：US09034584B2
公开(公告)日：2015-05-19
申请号：US13560159
申请日：2012-07-27
IPC分类号： G01N33/567 , C12Q1/00 , C12N5/077
CPC分类号： C12N5/0657 , C12N2500/25 , C12N2500/32 , C12N2500/33 , C12N2500/36 , C12N2500/90 , C12N2500/99
摘要： The present invention concerns methods and compositions for promoting a conversion in stem cells or cells derived therefrom from glycolysis to aerobic respiration and its associated pathways, including oxidative phosphorylation, the TCA cycle, fatty acid oxidation, and pyruvate decarboxylation, for example. In specific embodiments, certain media is employed to improve metabolic maturation of particular stem cell-derived cells, including human embryonic stem cells and human induced pluripotent stem cells, for example. Particular media comprises media without glucose or optionally without pyruvate, with fatty acids, with L-carnitine, with taurine, creatine, non-essential amino acids, L-glutamine, and with anti-oxidants or free-radical scavengers, for example.
摘要翻译： 本发明涉及用于促进干细胞或从其衍生的糖酵解转化成有氧呼吸及其相关途径（包括氧化磷酸化，TCA循环，脂肪酸氧化和丙酮酸脱羧）的转化方法和组合物。 在具体实施方案中，使用某些培养基来改善特定干细胞衍生细胞（包括人胚胎干细胞和人诱导的多能干细胞）的代谢成熟。 特别的培养基包括不含葡萄糖或任选不含丙酮酸的培养基，脂肪酸与L-肉毒碱，牛磺酸，肌酸，非必需氨基酸，L-谷氨酰胺，以及抗氧化剂或自由基清除剂。
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公开(公告)号：US20130029368A1
公开(公告)日：2013-01-31
申请号：US13560159
申请日：2012-07-27
CPC分类号： C12N5/0657 , C12N2500/25 , C12N2500/32 , C12N2500/33 , C12N2500/36 , C12N2500/90 , C12N2500/99
摘要： The present invention concerns methods and compositions for promoting a conversion in stem cells or cells derived therefrom from glycolysis to aerobic respiration and its associated pathways, including oxidative phosphorylation, the TCA cycle, fatty acid oxidation, and pyruvate decarboxylation, for example. In specific embodiments, certain media is employed to improve metabolic maturation of particular stem cell-derived cells, including human embryonic stem cells and human induced pluripotent stem cells, for example. Particular media comprises media without glucose or optionally without pyruvate, with fatty acids, with L-carnitine, with taurine, creatine, non-essential amino acids, L-glutamine, and with anti-oxidants or free-radical scavengers, for example.
摘要翻译： 本发明涉及用于促进干细胞或从其衍生的糖酵解转化成有氧呼吸及其相关途径（包括氧化磷酸化，TCA循环，脂肪酸氧化和丙酮酸脱羧）的转化方法和组合物。 在具体实施方案中，使用某些培养基来改善特定干细胞衍生细胞（包括人胚胎干细胞和人诱导的多能干细胞）的代谢成熟。 特别的培养基包括不含葡萄糖或任选不含丙酮酸的培养基，脂肪酸与L-肉毒碱，牛磺酸，肌酸，非必需氨基酸，L-谷氨酰胺，以及抗氧化剂或自由基清除剂。
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