公开(公告)号：US06784289B2

公开(公告)日：2004-08-31

申请号：US09747007

申请日：2000-12-21

申请人： Thérèse Ouellet ,  Brian M. Miki ,  Elizabeth Foster ,  Teresa Martin-Heller ,  Lining Tian ,  Daniel C. Brown ,  Peijun Zhang ,  Jiro Hattori ,  Kamal Malik ,  Keqiang Wu ,  David A. Theilmann ,  Raymond Tropiano

发明人： Thérèse Ouellet ,  Brian M. Miki ,  Elizabeth Foster ,  Teresa Martin-Heller ,  Lining Tian ,  Daniel C. Brown ,  Peijun Zhang ,  Jiro Hattori ,  Kamal Malik ,  Keqiang Wu ,  David A. Theilmann ,  Raymond Tropiano

IPC分类号： C07H2104

CPC分类号： C12N15/67 ,  C12N15/8216

摘要： The present invention is directed to translational regulatory elements that mediate the amount of protein produced within a host capable of expressing a construct comprising one or more translational regulatory elements in operative association with a gene of interest. These translational regulatory elements were derived from T1275 (tCUP) and exhibit a high degree of similarity with members of the RENT family of repetitive elements. Translational regulatory elements are disclosed that either increase or decrease he amount of protein produced within the host organism. These translational elements are operative in a wide range of hosts including plant, animals, yeast, fungi and bacteria. Analogs, derivatives and fragments of these translational elements are also disclosed.

摘要翻译： 本发明涉及介导在能够表达包含与感兴趣的基因有效结合的一种或多种翻译调控元件的构建体的宿主内产生的蛋白质的量的翻译调控元件。 这些翻译调控元件来自T1275（tCUP），并且与RENT家族的重复元件的成员呈现高程度的相似性。 公开了翻译调控元件，即增加或减少宿主生物体内产生的蛋白质的量。 这些翻译元件在广泛的宿主中可操作，包括植物，动物，酵母，真菌和细菌。 还公开了这些翻译元件的类似物，衍生物和片段。