公开(公告)号：US07303873B2

公开(公告)日：2007-12-04

申请号：US10437261

申请日：2003-05-13

申请人： Brian Miki ,  Thérèse Ouellet ,  Jiro Hattori ,  Elizabeth Foster ,  Hélène Labbé ,  Teresa Martin-Heller ,  Lining Tian ,  Daniel Charles William Brown ,  Peijun Zhang ,  Keqiang Wu

发明人： Brian Miki ,  Thérèse Ouellet ,  Jiro Hattori ,  Elizabeth Foster ,  Hélène Labbé ,  Teresa Martin-Heller ,  Lining Tian ,  Daniel Charles William Brown ,  Peijun Zhang ,  Keqiang Wu

IPC分类号： C12Q1/68 ,  C12N15/63 ,  C12N15/82 ,  C07H21/02 ,  C12N5/04

CPC分类号： C07K14/415 ,  C12N15/8216 ,  C12N15/8222 ,  C12N15/8234

摘要： T-DNA tagging with a promoterless β-glucuronidase (GUS) gene generated transgenic Nicotiana tabacum plants that expressed GUS activity either only in developing seed coats, or constitutively. Cloning and deletion analysis of the GUS fusion revealed that the promoter responsible for seed coat specificity was located in the plant DNA proximal to the GUS gene. Analysis of the region demonstrated that the seed coat-specificity of GUS expression in this transgenic plant resulted from T-DNA insertion next to a cryptic promoter. This promoter is useful in controlling the expression of genes to the developing seed coat in plant seeds. Similarly, cloning and characterization of the cryptic constitutive promoter revealed the occurrence of several cryptic regulatory regions. These regions include promoter, negative regulatory elements, transcriptional enhancers, core promoter regions, and translational enhancers and other regulatory elements.

摘要翻译： 用无启动子β-葡糖醛酸糖苷酶（GUS）基因的T-DNA标签产生转基因烟草植物，其仅在开发种皮或组成型中表达GUS活性。 GUS融合物的克隆和缺失分析表明，负责种衣特异性的启动子位于植物DNA接近GUS基因的位置。 该区域的分析表明，该转基因植物中GUS表达的种皮特异性是由隐匿启动子旁边的T-DNA插入产生的。 该启动子可用于控制植物种子中发育中的种皮的基因表达。 类似地，隐匿性组成型启动子的克隆和表征揭示了几个隐蔽调节区的发生。 这些区域包括启动子，负调控元件，转录增强子，核心启动子区和翻译增强子等调控元件。

公开(公告)号：US5756324A

公开(公告)日：1998-05-26

申请号：US625198

申请日：1996-04-01

申请人： Chris Baszczynski ,  Eric Barbour ,  Brian Miki ,  Jiro Hattori

发明人： Chris Baszczynski ,  Eric Barbour ,  Brian Miki ,  Jiro Hattori

IPC分类号： C07K14/415 ,  C12N15/29 ,  C12N15/82 ,  C12N15/32 ,  C12N15/33

CPC分类号： C07K14/415 ,  C12N15/8222 ,  C12N15/8286 ,  C12N15/8289

摘要： A novel DNA regulatory element that confers microspore-specific gene expression has been discovered, isolated, and characterized. The microspore-specific regulatory element can be used to control the expression of a foreign gene that disrupts the function of microspores. Thus, the control of pollen production can be achieved by using the microspore-specific regulatory element to produce male-sterile plants. Various methods can be used to restore male fertility in the F1 generation of such male-sterile plants. In addition, the microspore-specific regulatory element can be used to confer resistance to viral and insect pests.

摘要翻译： 已经发现，分离和表征赋予小孢子特异性基因表达的新型DNA调节元件。 小孢子特异性调节元件可用于控制破坏小孢子功能的外源基因的表达。 因此，可以通过使用小孢子特异性调节元件来产生雄性不育植物来实现对花粉产生的控制。 可以使用各种方法来恢复F1代这种雄性不育植物中的雄性生育力。 此外，小孢子特异性调节元件可用于赋予对病毒和昆虫害虫的抗性。

公开(公告)号：US5633438A

公开(公告)日：1997-05-27

申请号：US345756

申请日：1994-11-22

申请人： Chris Baszczynski ,  Eric Barbour ,  Brian Miki ,  Jiro Hattori

发明人： Chris Baszczynski ,  Eric Barbour ,  Brian Miki ,  Jiro Hattori

IPC分类号： C07K14/415 ,  C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  A01H1/02

CPC分类号： C07K14/415 ,  C12N15/8222 ,  C12N15/8286 ,  C12N15/8289

摘要： A novel DNA regulatory element that confers microspore-specific gene expression has been discovered, isolated, and characterized. The microspore-specific regulatory element can be used to control the expression of a foreign gene that disrupts the function of microspores. Thus, the control of pollen production can be achieved by using the microspore-specific regulatory element to produce male-sterile plants. Various methods can be used to restore male fertility in the F1 generation of such male-sterile plants. In addition, the microspore-specific regulatory element can be used to confer resistance to viral and insect pests.

摘要翻译： 已经发现，分离和表征赋予小孢子特异性基因表达的新型DNA调节元件。 小孢子特异性调节元件可用于控制破坏小孢子功能的外源基因的表达。 因此，可以通过使用小孢子特异性调节元件来产生雄性不育植物来实现对花粉产生的控制。 可以使用各种方法来恢复F1代这种雄性不育植物中的雄性生育力。 此外，小孢子特异性调节元件可用于赋予对病毒和昆虫害虫的抗性。

公开(公告)号：US07396978B2

公开(公告)日：2008-07-08

申请号：US10861875

申请日：2004-06-04

申请人： Brian Miki ,  Mark Gijzen ,  Shea Miller

发明人： Brian Miki ,  Mark Gijzen ,  Shea Miller

IPC分类号： C12N15/82 ,  C12N15/29 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N9/0065 ,  C07K14/415 ,  C12N15/8216 ,  C12N15/8234

摘要： The present invention is directed to a nucleic acid comprising a nucleotide sequence that encodes a protein that imparts a dull luster to a seed coat when expressed within seed-coat tissues and to transgenic seeds, plant cells and plants expressing the protein.

摘要翻译： 本发明涉及一种核酸，其包含编码当在种皮 - 外壳组织内表达时向种皮施用钝性光泽的蛋白质的核苷酸序列和表达该蛋白质的转基因种子，植物细胞和植物。

公开(公告)号：US07151170B1

公开(公告)日：2006-12-19

申请号：US09980364

申请日：2000-06-02

申请人： Kim Boutilier ,  Therese Ouellet ,  Jan Custers ,  Jiro Hattori ,  Brian Miki ,  Michiel Van Lookeren Campagne

发明人： Kim Boutilier ,  Therese Ouellet ,  Jan Custers ,  Jiro Hattori ,  Brian Miki ,  Michiel Van Lookeren Campagne

IPC分类号： C12N15/29

CPC分类号： C12N15/8261 ,  C12N15/8209 ,  C12N15/8231 ,  Y02A40/146

摘要： The present invention provides for a gene obtained during the induction of microspore embryogenisis. The protein encoded by this gene renders plant cells embryongenic, and increases the regenerative capacity of the plant cell. Also disclosed is the regulatory region of this gene and its use for directing the expression of a gene of interest within a suitable host cell.

摘要翻译： 本发明提供了在诱导小孢子胚发生过程中获得的基因。 由该基因编码的蛋白质使植物细胞胚胎发育，并增加植物细胞的再生能力。 还公开了该基因的调节区及其用于在合适的宿主细胞内引导感兴趣的基因的表达的用途。

公开(公告)号：US20050034195A1

公开(公告)日：2005-02-10

申请号：US10861875

申请日：2004-06-04

申请人： Brian Miki ,  Mark Gijzen ,  Shea Miller

发明人： Brian Miki ,  Mark Gijzen ,  Shea Miller

IPC分类号： C07K14/415 ,  C12N9/08 ,  C12N15/29 ,  C12N15/82 ,  A01H5/10

CPC分类号： C12N9/0065 ,  C07K14/415 ,  C12N15/8216 ,  C12N15/8234

摘要： The present invention is directed to an isolated genomic sequence that is differentially expressed within seed-coat tissues. This invention also relates to a promoter obtained from the genomic sequence that is differentially expressed in seed-coat tissues, and the use of this promoter for directing seed-coat specific expression of a gene of interest within transformed plant cells or plants. A method for modifying the luster of a seed coat is also provided.

摘要翻译： 本发明涉及在种皮组织内差异表达的分离的基因组序列。 本发明还涉及从在种皮组织中差异表达的基因组序列获得的启动子，以及该启动子在转化的植物细胞或植物中指导目的基因的特异性表达的目的基因的用途。 还提供了改变种皮的光泽的方法。

公开(公告)号：US5824872A

公开(公告)日：1998-10-20

申请号：US593121

申请日：1996-02-01

申请人： Brian Miki ,  Jiro Hattori ,  Pierre Fobert ,  Venkatran N. Iyer

发明人： Brian Miki ,  Jiro Hattori ,  Pierre Fobert ,  Venkatran N. Iyer

IPC分类号： C07K14/415 ,  C12N15/82 ,  A01H5/00 ,  C07H21/04 ,  C12N15/63

CPC分类号： C07K14/415 ,  C12N15/8216

摘要： T-DNA tagging with a promoterless .beta.-glucuronidase (GUS) gene generated a transgenic Nicotiana tabacum plant that expressed GUS activity constitutively. The gene fusion has been cloned and sequenced. It has been re-inserted into N. tabacum by Agrobacterium-mediated transformation. The N. tabacum DNA upstream from the GUS gene was approximately 2 kb in length and showed no homology to known sequences. This DNA, which contains a constitutive promoter, is useful in controlling the expression of exogenous genes in transgenic plants of diverse plant species.

摘要翻译： 用无启动子β-葡糖醛酸糖苷酶（GUS）基因的T-DNA标签产生了组成型表达GUS活性的转基因烟草植物。 基因融合已被克隆并测序。 通过农杆菌介导的转化将其重新插入到烟草中。 GUS基因上游的烟草DNA长度约为2 kb，与已知序列无同源性。 含有组成型启动子的该DNA可用于控制不同植物物种的转基因植物中外源基因的表达。

公开(公告)号：US06939958B1

公开(公告)日：2005-09-06

申请号：US10110637

申请日：2000-10-13

申请人： John Simmonds ,  Leslie Cass ,  Linda Harris ,  Sharon Allard ,  Kamal Malik ,  Teresa Martin-Heller ,  Dan Brown ,  Ming Hu ,  Brian Miki ,  Keqiang Wu

发明人： John Simmonds ,  Leslie Cass ,  Linda Harris ,  Sharon Allard ,  Kamal Malik ,  Teresa Martin-Heller ,  Dan Brown ,  Ming Hu ,  Brian Miki ,  Keqiang Wu

IPC分类号： C07K14/415 ,  C12N15/82 ,  C07H21/04

CPC分类号： C12N15/8234 ,  C07K14/415

摘要： This invention is directed to a regulatory region obtained from a wheat aleurone gene LtpW1. This regulatory region, truncated derivatives, mutations, or deletions of this regulatory region, can be used to express heterologous genes of interest within aleurone cells of a plant. Furthermore, this invention is directed to a truncated LtpW1 regulatory region that exhibits constitutive activity with both monocot and dicot plants. This invention is also directed to vectors comprising these regulatory regions operatively linked with a heterologous gene of interest, as well as plant cell cultures and transgenic plants comprising these vectors. A method for the preparation of a plant using the regulatory regions of this invention are also disclosed.

摘要翻译： 本发明涉及从小麦糊粉基因LtpW1获得的调节区。 该调节区域，该调节区的截短的衍生物，突变或缺失可用于在植物的糊粉母细胞内表达感兴趣的异源基因。 此外，本发明涉及一种截短的LtpW1调节区，其表现出单子叶植物和双子叶植物的组成活性。 本发明还涉及包含与感兴趣的异源基因可操作地连接的这些调节区的载体，以及包含这些载体的植物细胞培养物和转基因植物。 还公开了使用本发明的调节区制备植物的方法。

公开(公告)号：US20050055742A1

公开(公告)日：2005-03-10

申请号：US10866529

申请日：2004-06-10

申请人： Brian Miki ,  Jiro Hattori ,  Teresa Martin-Heller ,  Helene Labbe ,  Kamal Malik ,  Elizabeth Foster ,  Keqiang Wu ,  Daniel Brown ,  Lining Tian ,  Therese Ouellet ,  Peijun Zhang ,  Elizabeth James ,  Pierre Fobert ,  Venkatram Iyer

发明人： Brian Miki ,  Jiro Hattori ,  Teresa Martin-Heller ,  Helene Labbe ,  Kamal Malik ,  Elizabeth Foster ,  Keqiang Wu ,  Daniel Brown ,  Lining Tian ,  Therese Ouellet ,  Peijun Zhang ,  Elizabeth James ,  Pierre Fobert ,  Venkatram Iyer

IPC分类号： C07K14/415 ,  C12N15/82 ,  C12Q1/68 ,  A01H1/00 ,  C07H21/04 ,  C12N5/04

CPC分类号： C07K14/415 ,  C12N15/8216

摘要： An nucleotide sequence and that exhibits regulatory element activity is disclosed. The nucleotide sequence may be defined by SEQ ID NO:22, a nucleotide sequence that hybridizes to the nucleic acid sequence of SEQ ID NO:22, or a compliment thereof. Also disclosed is a chimeric construct comprising the nucleotide sequence operatively linked with a coding region of interest. A method of expressing a coding region of interest within a plant by introducing the chimeric construct described above, into the plant, and expressing the coding region of interest is also provided. Also disclosed are plants, seed, or plant cells comprising the chimeric construct as defined above.

摘要翻译： 公开了核苷酸序列并表现出调节元件活性。 核苷酸序列可以由SEQ ID NO：22定义，与SEQ ID NO：22的核酸序列杂交的核苷酸序列或其补体。 还公开了包含与感兴趣的编码区可操作地连接的核苷酸序列的嵌合构建体。 还提供了通过将上述嵌合构建体引入植物并表达感兴趣的编码区而在植物内表达感兴趣的编码区的方法。 还公开了包含如上定义的嵌合构建体的植物，种子或植物细胞。

公开(公告)号：US5824863A

公开(公告)日：1998-10-20

申请号：US441597

申请日：1995-05-15

申请人： Brian Miki ,  V. N. Iyer ,  Pierre Fobert

发明人： Brian Miki ,  V. N. Iyer ,  Pierre Fobert

IPC分类号： C12N15/82 ,  A01H5/00

CPC分类号： C12N15/8234

摘要： T-DNA tagging with a promoterless .beta.-glucuronidase (GUS) gene generated a transgenic Nicotiana tabacum plant that expressed GUS activity only in developing seed coats. Cloning and deletion analysis of the GUS fusion revealed that the promoter responsible for seed coat specificity was located in the plant DNA proximal to the GUS gene. Analysis of the region demonstrated that the seed coat-specificity of GUS expression in this transgenic plant resulted from T-DNA insertion next to a cryptic promoter. This promotor is useful in controlling the expression of genes to the developing seed coat in plant seeds.

摘要翻译： 用无启动子β-葡糖醛酸糖苷酶（GUS）基因的T-DNA标签产生转基因烟草植物，其仅在开发种皮中表达GUS活性。 GUS融合物的克隆和缺失分析表明，负责种衣特异性的启动子位于植物DNA接近GUS基因的位置。 该区域的分析表明，该转基因植物中GUS表达的种皮特异性是由隐匿启动子旁边的T-DNA插入产生的。 该启动子可用于控制植物种子中发育中的种皮的基因表达。