公开(公告)号：US20130094755A1

公开(公告)日：2013-04-18

申请号：US13705237

申请日：2012-12-05

申请人： Carl Zeiss Microlmaging GmbH

发明人： Helmut Lippert ,  Benno Radt ,  Michael Kempe ,  Christian Dietrich

IPC分类号： G06T7/00

CPC分类号： G06T7/97 ,  G02B21/002 ,  G02B21/244 ,  G02B21/367

摘要： A method for the three-dimensional imaging of a sample in which image information from different depth planes of the sample is stored in a spatially resolved manner, and the three-dimensional image of the sample is subsequently reconstructed from this stored image information is provided. A reference structure is applied to the illumination light, at least one fluorescing reference object is positioned next to or in the sample, images of the reference structure of the illumination light, of the reference object are recorded from at least one detection direction and evaluated. The light sheet is brought into an optimal position based on the results and image information of the reference object and of the sample from a plurality of detection directions is stored. Transformation operators are obtained on the basis of the stored image information and the reconstruction of the three-dimensional image of the is based on these transformation operators.

摘要翻译： 提供了一种用于样本的三维成像的方法，其中以空间分辨的方式存储来自样本的不同深度平面的图像信息，并且随后从该存储的图像信息重建样本的三维图像。 参考结构被应用于照明光，至少一个发荧光参考对象被放置在样本旁边或样本中，从至少一个检测方向记录参考物体的照明光的参考结构的图像并进行评估。 基于结果使光照片进入最佳位置，存储参考对象的图像信息和来自多个检测方向的样本的图像信息。 基于存储的图像信息和基于这些变换算子的三维图像的重建获得转换算子。

公开(公告)号：US20130329284A1

公开(公告)日：2013-12-12

申请号：US13966714

申请日：2013-08-14

申请人： Carl Zeiss Microlmaging GmbH

发明人： Michael SCHWERTNER ,  Ralf Wolleschensky ,  Michael Kempe

IPC分类号： G02B21/06

CPC分类号： G02B21/14 ,  G02B21/0024 ,  G02B21/06

摘要： A method for the optical detection of an illuminated specimen, wherein the illuminating light impinges in a spatially structured manner in at least one plane on the specimen and several images of the specimen are acquired by a detector in different positions of the structure on the specimen. An optical sectional image and/or an image with enhanced resolution is then calculated. The method includes generating a diffraction pattern in the direction of the specimen in or near the pupil of the objective lens or in a plane conjugate to the pupil. A phase plate with regions of varying phase delays is dedicated to the diffraction pattern in or near the pupil of the objective lens or in a plane conjugate to said pupil, and different phase angles of the illuminating light are set.

摘要翻译： 一种用于照射样本的光学检测的方法，其中所述照明光以空间结构的方式照射在所述样本上的至少一个平面中，并且所述样本的多个图像由所述样本上的所述结构的不同位置的检测器获取。 然后计算出具有增强的分辨率的光学截面图像和/或图像。 该方法包括在物镜的瞳孔中或其附近或在与瞳孔共轭的平面中的样本的方向上产生衍射图案。 具有不同相位延迟的区域的相位板专用于物镜的光瞳中或附近的衍射图案或与所述瞳孔共轭的平面，并且设定照明光的不同相位角。