公开(公告)号：US20050124063A1

公开(公告)日：2005-06-09

申请号：US10839212

申请日：2004-05-06

申请人： Mei-Ju Yang ,  Kuang-Ning Chang ,  Lih-Tao Hsu ,  Jun-Jae Huang ,  Wann-Hsin Chen ,  Chao-Ying Kuo

发明人： Mei-Ju Yang ,  Kuang-Ning Chang ,  Lih-Tao Hsu ,  Jun-Jae Huang ,  Wann-Hsin Chen ,  Chao-Ying Kuo

IPC分类号： C12M3/00 ,  C12N5/00 ,  C12N5/02 ,  C12N5/0735 ,  C12N5/08

CPC分类号： C12N5/0606 ,  C12N2500/84 ,  C12N2502/13 ,  C12N2533/90

摘要： The present invention discloses an improved culture system for proliferative and undifferentiated growth of human embryonic stem cells, comprising an extracellular matrix prepared from feeder cells and a conditioned medium preconditioned by feeder cells. This invention also relates to a culture method utilizing the foregoing culture system.

摘要翻译： 本发明公开了一种用于人胚胎干细胞增殖和未分化生长的改良培养系统，其包括由饲养细胞制备的细胞外基质和由饲养细胞预处理的条件培养基。 本发明还涉及利用上述培养系统的培养方法。

公开(公告)号：US07682826B2

公开(公告)日：2010-03-23

申请号：US11233055

申请日：2005-09-23

申请人： Mei Ju Yang ,  Kuang Ning Chang ,  Lih Tao Hsu ,  Jun Jae Huang ,  Wann Hsin Chen ,  Chao Ying Kuo

发明人： Mei Ju Yang ,  Kuang Ning Chang ,  Lih Tao Hsu ,  Jun Jae Huang ,  Wann Hsin Chen ,  Chao Ying Kuo

IPC分类号： C12N5/00

CPC分类号： C12N5/0606 ,  C12N2500/84 ,  C12N2502/13 ,  C12N2533/90

摘要： Provided herein are human embryonic stem cells and their method of culturing. In one embodiment, the human embryonic stem cells are maintained in an environment containing an extracellular matrix isolated from feeder cells and a conditioned medium pre-conditioned by the feeder cells; the feeder cells are pre-inactivated by either gamma ray radiation or by mitomycin C treatment. The cultured human embryonic stem cells remain substantially undifferentiated and maintain their pluripotency to differentiate into three germ layer cells.

摘要翻译： 本文提供人胚胎干细胞及其培养方法。 在一个实施方案中，将人胚胎干细胞保持在含有从饲养细胞分离的细胞外基质和由饲养细胞预处理的条件培养基的环境中; 饲养细胞通过γ射线辐射或丝裂霉素C处理预灭活。 培养的人胚胎干细胞保持基本上未分化，并保持其多能性以分化成三个胚层细胞。

公开(公告)号：US20060030040A1

公开(公告)日：2006-02-09

申请号：US11233055

申请日：2005-09-23

申请人： Mei-Ju Yang ,  Kuang-Ning Chang ,  Lih-Tao Hsu ,  Jun-Jae Huang ,  Wann-Hsin Chen ,  Chao-Ying Kuo

发明人： Mei-Ju Yang ,  Kuang-Ning Chang ,  Lih-Tao Hsu ,  Jun-Jae Huang ,  Wann-Hsin Chen ,  Chao-Ying Kuo

IPC分类号： C12N5/08 ,  C12N5/06

CPC分类号： C12N5/0606 ,  C12N2500/84 ,  C12N2502/13 ,  C12N2533/90

摘要： Provided herein are human embryonic stem cells and their method of culturing. In one embodiment, the human embryonic stem cells are maintained in an environment containing an extracellular matrix isolated from feeder cells and a conditioned medium pre-conditioned by the feeder cells; the feeder cells are pre-inactivated by either gamma ray radiation or by mitomycin C treatment. The cultured human embryonic stem cells remain substantially undifferentiated and maintain their pluripotency to differentiate into three germ layer cells.

摘要翻译： 本文提供人胚胎干细胞及其培养方法。 在一个实施方案中，将人胚胎干细胞保持在含有从饲养细胞分离的细胞外基质和由饲养细胞预处理的条件培养基的环境中; 饲养细胞通过γ射线辐射或丝裂霉素C处理预灭活。 培养的人胚胎干细胞保持基本上未分化，并保持其多能性以分化成三个胚层细胞。