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公开(公告)号：US07739055B2
公开(公告)日：2010-06-15
申请号：US11561266
申请日：2006-11-17
IPC分类号： G06F19/00
CPC分类号： C07K7/08 , A61K38/00 , C07K14/001 , C07K14/4723 , C40B50/02 , G06F19/18 , G06F19/22 , G06F19/24 , G06F19/28
摘要： Methods to create databases of peptides having a desirable property, such as antimicrobial activity, involving analyzing a database of known peptides for a pattern statistically associated with the desirable property are described herein, The set of sequences being analyzed may include sequences of a desired length containing all or substantially all combinations of amino acids that conform to at least one of the set of patterns. Once the database is identified, the database may be processed in a pattern recognition procedure that identifies a set of patterns that may be representative of a peptide having the desirable property. A set of newly generated peptides sequences may then be processed to score these new sequences against the identified patterns to correlate the patterns to the sequences and determine a degree of association or similarity between one or more of the new sequences and the set of identified patterns.
摘要翻译： 本文描述了创建具有期望性质（例如抗微生物活性）的肽的数据库的方法，其涉及分析已知肽的数据库以获得与所需性质统计学相关的模式。所分析的一组序列可以包含期望长度的序列，其含有 所有或基本上所有符合该组图案中的至少一个的氨基酸组合。 一旦识别了数据库，就可以在模式识别程序中处理数据库，该模式识别程序识别可以代表具有所需性质的肽的一组模式。 然后可以处理一组新产生的肽序列以针对所识别的模式对这些新序列进行评估，以将模式与序列相关联，并确定一个或多个新序列与所识别的模式集合之间的关联度或相似度。
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2.发明申请公开(公告)号：US20070254006A1
公开(公告)日：2007-11-01
申请号：US11675500
申请日：2007-02-15
申请人： Christopher Loose , William O'Shaughnessy , LinO Ferreira , Andreas Zumbuehl , Robert Langer , Gregory Stephanopoulos
发明人： Christopher Loose , William O'Shaughnessy , LinO Ferreira , Andreas Zumbuehl , Robert Langer , Gregory Stephanopoulos
CPC分类号： A61L27/54 , A61K38/10 , A61L2/16 , A61L2/232 , A61L15/46 , A61L27/227 , A61L27/34 , A61L29/085 , A61L31/10 , A61L31/16 , A61L2300/25 , A61L2300/404
摘要： Antimicrobial peptides enable an alternate approach to developing antimicrobial coatings due to their targeting of the membranes of the bacteria. High specific activity is achieved by orienting the peptides so that the antimicrobial ends of the peptides maximally contact the bacteria. In one embodiment, one end of the peptide is covalently attached directly to the substrate. In another embodiment, the peptides are immobilized on the substrate using a coupling agent or tether. Non-covalent methods include coating the peptide onto the substrate or physiochemically immobilizing the peptides on the substrate using highly specific interactions, such as the biotin/avidin or streptavidin system. The compositions are substantially non-leaching, antifouling, and non-hemolytic. The immobilized peptides retain sufficient flexibility and mobility to interact with and de endocytosed by the bacteria, viruses, and/or fungi upon exposure. Immobilizing the peptides to the substrate reduces concerns regarding toxicity of the peptides and the development of antimicrobial resistance, while presenting substantially all of the peptide at the site of action at the surface of the substrate.
摘要翻译： 由于抗菌肽靶向细菌膜，抗微生物肽能够开发抗微生物涂层。 通过使肽定向以使肽的抗微生物末端最大程度地接触细菌来实现高比活性。 在一个实施方案中，肽的一端直接共价连接到底物上。 在另一个实施方案中，使用偶联剂或系链将肽固定在底物上。 非共价方法包括将肽涂覆到底物上或者使用高度特异性相互作用如生物素/抗生物素蛋白或链霉亲和素系统在肽上生理化学地固定在底物上。 组合物基本上是非浸出，防污和非溶血性的。 固定的肽保持足够的灵活性和移动性，以在暴露时与细菌，病毒和/或真菌相互作用和内吞。 将肽固定到底物可减少对肽的毒性和抗微生物耐药性的发展的担忧，同时在基底表面的作用部位呈现基本上所有的肽。
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公开(公告)号：US20070197773A1
公开(公告)日：2007-08-23
申请号：US11561266
申请日：2006-11-17
IPC分类号： G06F19/00 , C07K14/16 , C07K14/195
CPC分类号： C07K7/08 , A61K38/00 , C07K14/001 , C07K14/4723 , C40B50/02 , G06F19/18 , G06F19/22 , G06F19/24 , G06F19/28
摘要： A method has been developed to create databases of peptides having a desirable property, such as antimicrobial activity, based on analyzing a database of known peptides for a pattern statistically associated with an activity. One can determine a set of patterns that may be representative of a peptide having a desired characteristic or property, and evaluate a set of sequences against the set of patterns (grammars) to determine if the peptide sequence being evaluated has similar patterns to those of a peptide having the desired characteristic or property. The set of sequences being evaluated may include peptide sequences of a desired length comprising all or substantially all combinations of amino acids that conform to at least one of the set of patterns. Once the database is identified the database may be processed in a pattern recognition procedure that identifies a set of patterns that could be understood as representative of a peptide having the characteristic of interest. A set of newly generated peptides sequences may then be processed to score these new sequences against the identified patterns to correlate the patterns to the sequences and determine a degree of association or a similarity between a respective one of the new sequences and the set of identified patterns. The method is used to provide a database of sequences that are expected to have one or more desired activities, specific sequences within the database proven to have the desired activity, and the patterns or grammars used to create the database of sequences. Although described with reference to antimicrobial peptides, a database of peptides may be identified that contains peptides that have antiviral properties, wound response properties, or some other property of interest.
摘要翻译： 已经开发了一种基于分析与活性统计相关的模式的已知肽的数据库来创建具有所需性质（例如抗微生物活性）的肽的数据库的方法。 可以确定可以代表具有期望特征或性质的肽的一组模式，并且针对该组模式（语法）评估一组序列，以确定被评估的肽序列是否具有与 肽具有所需的特性或性质。 正在评估的序列集合可以包括期望长度的肽序列，其包含符合该组模式中的至少一个的氨基酸的全部或基本上全部组合。 一旦数据库被识别，数据库可以在识别可以被理解为具有感兴趣特征的肽的代表的一组模式的模式识别过程中被处理。 然后可以对一组新产生的肽序列进行处理，以针对所识别的模式对这些新序列进行评估，以将模式与序列相关联，并确定新序列中的相应一个和所识别的模式集合之间的关联程度或相似度 。 该方法用于提供预期具有一个或多个期望活动的序列的数据库，被证明具有期望活动的数据库中的特定序列，以及用于创建序列数据库的模式或语法。 尽管参考抗微生物肽描述了，但是可以鉴定出含有具有抗病毒性质，伤口应答性质或感兴趣的其它性质的肽的肽数据库。
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公开(公告)号：US20100184693A1
公开(公告)日：2010-07-22
申请号：US12685522
申请日：2010-01-11
CPC分类号： C07K7/08 , A61K38/00 , C07K14/001 , C07K14/4723 , G16B20/00 , G16B30/00 , G16B35/00 , G16B40/00 , G16B50/00 , G16C20/60
摘要： A method has been developed to create databases of peptides having a desirable property, such as antimicrobial activity, based on analyzing a database of known peptides for a pattern statistically associated with an activity. One can determine a set of patterns that may be representative of a peptide having a desired characteristic or property, and evaluate a set of sequences against the set of patterns (grammars) to determine if the peptide sequence being evaluated has similar patterns to those of a peptide having the desired characteristic or property. The set of sequences being evaluated may include peptide sequences of a desired length comprising all or substantially all combinations of amino acids that conform to at least one of the set of patterns. Once the database is identified, the database may be processed in a pattern recognition procedure that identifies a set of patterns that could be understood as representative of a peptide having the characteristic of interest. A set of newly generated peptides sequences may then be processed to score these new sequences against the identified patterns to correlate the patterns to the sequences and determine a degree of association or a similarity between a respective one of the new sequences and the set of identified patterns. The method is used to provide a database of sequences that are expected to have one or more desired activities, specific sequences within the database proven to have the desired activity, and the patterns or grammars used to create the database of sequences. Although described with reference to antimicrobial peptides, a database of peptides may be identified that contains peptides that have antiviral properties, wound response properties, or some other property of interest.
摘要翻译： 已经开发了一种基于分析与活性统计相关的模式的已知肽的数据库来创建具有所需性质（例如抗微生物活性）的肽的数据库的方法。 可以确定可以代表具有期望特征或性质的肽的一组模式，并且针对该组模式（语法）评估一组序列，以确定被评估的肽序列是否具有与 肽具有所需的特性或性质。 正在评估的序列集合可以包括期望长度的肽序列，其包含符合该组模式中的至少一个的氨基酸的全部或基本上全部组合。 一旦数据库被识别，数据库就可以在模式识别程序中进行处理，该模式识别程序识别可以被理解为代表具有感兴趣特征的肽的一组模式。 然后可以对一组新产生的肽序列进行处理，以针对所识别的模式对这些新序列进行评估，以将模式与序列相关联，并确定新序列中的相应一个和所识别的模式集合之间的关联程度或相似度 。 该方法用于提供预期具有一个或多个期望活动的序列的数据库，被证明具有期望活动的数据库中的特定序列，以及用于创建序列数据库的模式或语法。 尽管参考抗微生物肽描述了，但是可以鉴定出含有具有抗病毒性质，伤口应答性质或感兴趣的其它性质的肽的肽数据库。
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