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公开(公告)号:US20100261179A1
公开(公告)日:2010-10-14
申请号:US12682123
申请日:2008-10-17
申请人: Jason Betley , Barry Boyes , John Walter Czajka , Michael Roy Fairs , Colin Fewster , Thomas David Ford , Douglas Jason Green , Piers Sebastian Harding , Gary Stephen Howard , Jay Lewington , William Richard Mawer , Catherine Mills , James Onslow , Carmelo Volpe
发明人: Jason Betley , Barry Boyes , John Walter Czajka , Michael Roy Fairs , Colin Fewster , Thomas David Ford , Douglas Jason Green , Piers Sebastian Harding , Gary Stephen Howard , Jay Lewington , William Richard Mawer , Catherine Mills , James Onslow , Carmelo Volpe
CPC分类号: B03C1/288 , B03C2201/18 , B03C2201/26 , G01N35/0098 , G01N2035/00366
摘要: The application provides sample preparation devices and analyses. The devices and analyzes allow for the rapid preparation and analysis of samples using a variety of techniques, including PCR, by even unskilled users.
摘要翻译: 该应用程序提供样品制备装置和分析。 这些设备和分析允许甚至不熟练的用户使用各种技术(包括PCR)快速准备和分析样品。
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公开(公告)号:US08327724B2
公开(公告)日:2012-12-11
申请号:US12449855
申请日:2008-02-28
申请人: Michael Roy Fairs , Thomas David Ford , Piers Sebastian Harding , Gary Stephen Howard , Barry Boyes , John Walter Czajka , Douglas Jason Green , Jay Lewington , Carmelo Volpe , Colin Fewster , Jason Betley , Catherine Mills , William Richard Mawer
发明人: Michael Roy Fairs , Thomas David Ford , Piers Sebastian Harding , Gary Stephen Howard , Barry Boyes , John Walter Czajka , Douglas Jason Green , Jay Lewington , Carmelo Volpe , Colin Fewster , Jason Betley , Catherine Mills , William Richard Mawer
IPC分类号: G01N1/00
CPC分类号: G01N1/28 , B01L3/502 , B01L3/5082 , B01L2300/046 , B01L2300/0681 , B01L2300/087 , B01L2400/0683 , G01N2035/1086
摘要: Apparatus for preparing a biological sample for PCR analysis has an inlet (4) for receiving the sample (15) and a sieve (13) through which the sample is forced by screwing down an inlet cap (16). A lysis solution between two rupturable seals (11 and 12) is effective to disrupt the cells of the sample and release nucleic acid. The apparatus is mounted releasably on a PCR machine (2) having a motor (2′) releasably coupled with a drive mechanism (3, 36,40) in the apparatus to effect a rotary and a vertical up and down movement of its components (30, 31, 32, 42). The apparatus has a transparent cuvette (5) and a needle (71) extending to the lower end of the cuvette, by which the prepared sample is dispensed to the cuvette for PCR analysis.
摘要翻译: 用于制备用于PCR分析的生物样品的装置具有用于接收样品(15)的入口(4)和通过拧入入口盖(16)来迫使样品被迫的筛子。 两个可破裂的密封件(11和12)之间的裂解溶液有效地破坏样品的细胞并释放核酸。 该装置可释放地安装在具有可释放地与设备中的驱动机构(3,36,40)联接的马达(2')的PCR机(2)上,以实现其部件的旋转和垂直上下运动 30,31,32,42)。 该装置具有透明比色皿(5)和延伸到比色皿下端的针(71),通过该针将准备的样品分配到比色皿进行PCR分析。
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公开(公告)号:US20100192706A1
公开(公告)日:2010-08-05
申请号:US12449855
申请日:2008-02-28
申请人: Michael Roy Fairs , Thomas David Ford , Piers Sebastian Harding , Gary Stephen Howard , Barry Boyes , John Walter Czajka , Douglas Jason Green , Jay Lewington , Carmelo Volpe , Colin Fewster , Jason Betley , Catherine Mills , William Richard Mawer
发明人: Michael Roy Fairs , Thomas David Ford , Piers Sebastian Harding , Gary Stephen Howard , Barry Boyes , John Walter Czajka , Douglas Jason Green , Jay Lewington , Carmelo Volpe , Colin Fewster , Jason Betley , Catherine Mills , William Richard Mawer
IPC分类号: G01N1/10
CPC分类号: G01N1/28 , B01L3/502 , B01L3/5082 , B01L2300/046 , B01L2300/0681 , B01L2300/087 , B01L2400/0683 , G01N2035/1086
摘要: Apparatus for preparing a biological sample for PCR analysis has an inlet (4) for receiving the sample (15) and a sieve (13) through which the sample is forced by screwing down an inlet cap (16). A lysis solution between two rupturable seals (11 and 12) is effective to disrupt the cells of the sample and release nucleic acid. The apparatus is mounted releasably on a PCR machine (2) having a motor (2′) releasably coupled with a drive mechanism (3, 36,40) in the apparatus to effect a rotary and a vertical up and down movement of its components (30, 31, 32, 42). The apparatus has a transparent cuvette (5) and a needle (71) extending to the lower end of the cuvette, by which the prepared sample is dispensed to the cuvette for PCR analysis.
摘要翻译: 用于制备用于PCR分析的生物样品的装置具有用于接收样品(15)的入口(4)和通过拧入入口盖(16)来迫使样品被迫的筛子。 两个可破裂的密封件(11和12)之间的裂解溶液有效地破坏样品的细胞并释放核酸。 该装置可释放地安装在具有可释放地与设备中的驱动机构(3,36,40)联接的马达(2')的PCR机(2)上,以实现其部件的旋转和垂直上下运动 30,31,32,42)。 该装置具有透明比色皿(5)和延伸到比色皿下端的针(71),通过该针将准备的样品分配到比色皿进行PCR分析。
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