公开(公告)号：US20100291599A1

公开(公告)日：2010-11-18

申请号：US12467440

申请日：2009-05-18

申请人： Thomas J. Tague, JR. ,  William Kopaciewicz ,  Vincent J. Davisson ,  Elena Chernokalskaya ,  Timothy S. Rider ,  Cruz A.D. Hinojos ,  Jun Zhao

发明人： Thomas J. Tague, JR. ,  William Kopaciewicz ,  Vincent J. Davisson ,  Elena Chernokalskaya ,  Timothy S. Rider ,  Cruz A.D. Hinojos ,  Jun Zhao

IPC分类号： G01N33/53 ,  G01N21/65 ,  G01J3/44

CPC分类号： G01N21/658 ,  G01J3/44 ,  G01N21/65 ,  G01N33/54373

摘要： Raman spectra of protein immunoblots or enzyme linked immunosorbant assay procedures are acquired with a scanning Raman spectrometer. The sensitivity of the measurement is increased by conjugating secondary antibodies used in the Western blot and ELISA methods to surface enhanced Raman Scattering (SERS) labels. The resulting blot or well plate is analyzed with a Raman system that has forms a pixel map of the sample. More specifically, the Raman system generates an effectively line-shaped illumination pattern and scans the sample in the direction perpendicular to the line while the signal is accumulating on the detector. Each pixel is therefore a rectangle defined by the length of the illumination and the distance traveled by the sample within the duration of signal accumulation on the detector. The pixels are sequentially acquired to generate a map of the sample.

摘要翻译： 使用扫描拉曼光谱仪获得蛋白免疫印迹或酶联免疫吸附测定程序的拉曼光谱。 通过将Western印迹和ELISA方法中使用的二次抗体与表面增强的拉曼散射（SERS）标记缀合，来增加测量的灵敏度。 用形成样品的像素图的拉曼系统分析所得到的印迹或孔板。 更具体地，拉曼系统产生有效的线状照明图案，并且在信号积聚在检测器上的同时沿垂直于线的方向扫描样品。 因此，每个像素是由照明长度和样本在检测器上的信号累积持续时间内行进的距离所定义的矩形。 依次获取像素以生成样本的图。