公开(公告)号：US5923036A

公开(公告)日：1999-07-13

申请号：US797713

申请日：1997-02-11

申请人： Thomas J. Tague, Jr. ,  Norman Mortensen ,  Michael K. Bellamy

发明人： Thomas J. Tague, Jr. ,  Norman Mortensen ,  Michael K. Bellamy

IPC分类号： G01J3/28 ,  G01J3/457 ,  G02B21/24 ,  G01N21/35

CPC分类号： G01J3/2846 ,  G01J3/2823 ,  G01J3/457 ,  G02B21/24

摘要： An infrared imaging microscope uses spatial encoding to divide an sample being examined into a plurality of pixel regions. The spatial encoding is provided by a digitally controlled mask, which is preferably a multiple mirror array, and which masks the imaging radiation directed from a radiation source to the sample. The signal reflected or transmitted from the sample is detected using a single-element detector. As the mask pattern provided by the mask changes, the output signal of the detector is monitored, and the spectroscopic composition of each of the pixel regions is resolved using a spatial decoding method, such as a Hadamard transform. The digital control of the mask allows fast, easily-implemented changes to the masking pattern, and provides a low processing load relative to imaging devices that use multiple-element detectors. The invention may be implemented in a stand-alone microscope, or as a probe in which most of the elements of the device are located in a main housing, while the mask is located in a remote probe housing connected to the main housing by fiber optic cables. This allows reflective-mode scanning of free-standing objects. In one alternative embodiment, a multiple-element detector is used with the digitally-controlled mask. In this embodiment, visible light is spatially encoded by the mask along with the imaging radiation. By masking the visible light in this manner, visual examination of the sample allows correlation between the area of the sample being examined and the output of the appropriate element of the detector.

摘要翻译： 红外成像显微镜使用空间编码将待检样本分成多个像素区域。 空间编码由数字控制的掩模提供，其优选地是多反射镜阵列，并且将从辐射源引导的成像辐射掩蔽到样品。 使用单元件检测器检测从样品反射或发射的信号。 当由掩模提供的掩模图案改变时，监测检测器的输出信号，并且使用诸如Hadamard变换的空间解码方法来解析每个像素区域的分光组成。 掩模的数字控制允许对掩模图案的快速，容易实现的改变，并且相对于使用多元件检测器的成像装置提供低处理负载。 本发明可以在独立的显微镜中或作为探针中实现，其中装置的大多数元件位于主壳体中，而掩模位于通过光纤连接到主壳体的远程探测器壳体中 电缆 这允许对独立物体进行反射模式扫描。 在一个替代实施例中，多元素检测器与数字控制掩模一起使用。 在该实施例中，可见光与成像辐射一起由掩模进行空间编码。 通过以这种方式掩蔽可见光，样本的目视检查允许正在检查的样品的面积与检测器的适当元件的输出之间的相关性。

公开(公告)号：US20110058176A1

公开(公告)日：2011-03-10

申请号：US12263948

申请日：2008-11-03

申请人： Christian Pflugl ,  Benjamin G. Lee ,  Laurent Diehl ,  Mikhail A. Belkin ,  Federico Capasso ,  Thomas J. Tague, JR.

发明人： Christian Pflugl ,  Benjamin G. Lee ,  Laurent Diehl ,  Mikhail A. Belkin ,  Federico Capasso ,  Thomas J. Tague, JR.

IPC分类号： G01J3/45 ,  G01J5/02

CPC分类号： G01J3/108 ,  B82Y20/00 ,  G01J3/4338 ,  H01S5/024 ,  H01S5/0612 ,  H01S5/3402 ,  H01S5/4062 ,  H01S5/4087

摘要： A mid infrared spectrometer comprises a high brightness broadband source that generates an output with a broad spectral range in the order of hundreds of wave numbers, a wavelength dispersive element and a detector. In one embodiment, the source comprises an array of semiconductor laser devices operating simultaneously. Each device emits light at wavelength different from the wavelengths emitted by the other devices in the array and the devices are arranged so that the combined output continuously covers the broad spectral range. In another embodiment, each of the lasers in the array is a quantum cascade laser device. In still another embodiment, the quantum cascade laser devices in the array are operated in the regime of Risken-Nummedal-Graham-Haken (RNGH) instabilities. In yet another embodiment, each of the lasers in the array is a mode-locked quantum cascade laser device.

摘要翻译： 中红外光谱仪包括高亮度宽带源，其产生具有数百个波数量级的宽光谱范围的输出，波长色散元件和检测器。 在一个实施例中，源包括同时操作的半导体激光器件阵列。 每个器件发射波长不同于阵列中其他器件发射的波长的光，并且器件被布置成使得组合输出连续地覆盖宽光谱范围。 在另一个实施例中，阵列中的每个激光器是量子级联激光器件。 在又一个实施例中，阵列中的量子级联激光器件在Risken-Nummedal-Graham-Haken（RNGH）不稳定的状态下运行。 在另一个实施例中，阵列中的每个激光器是锁模量子级联激光器件。

公开(公告)号：US07787117B1

公开(公告)日：2010-08-31

申请号：US12145018

申请日：2008-06-24

申请人： Marco Leona ,  Thomas J. Tague, Jr.

发明人： Marco Leona ,  Thomas J. Tague, Jr.

IPC分类号： G01J3/44 ,  G01N21/65

CPC分类号： G01N21/658 ,  G01J3/44

摘要： In apparatus for performing Surface Enhanced Raman Spectroscopy (SERS), rather than applying a sample to be analyzed to an SERS active substrate, the SERS active substrate is applied to the sample using an inkjet nozzle to apply a substance containing a colloidal metal, such as silver, to the sample. The prepared sample is then analyzed with a Raman spectrometer in a conventional fashion.

摘要翻译： 在用于进行表面增强拉曼光谱（SERS）的装置中，使用喷墨喷嘴将SERS活性基板施加到样品上，而不是将待分析的样品施加到SERS活性基板上，以将含有胶体金属的物质 银，样品。 然后用拉曼光谱仪以常规方式分析制备的样品。

公开(公告)号：US20100291599A1

公开(公告)日：2010-11-18

申请号：US12467440

申请日：2009-05-18

申请人： Thomas J. Tague, JR. ,  William Kopaciewicz ,  Vincent J. Davisson ,  Elena Chernokalskaya ,  Timothy S. Rider ,  Cruz A.D. Hinojos ,  Jun Zhao

发明人： Thomas J. Tague, JR. ,  William Kopaciewicz ,  Vincent J. Davisson ,  Elena Chernokalskaya ,  Timothy S. Rider ,  Cruz A.D. Hinojos ,  Jun Zhao

IPC分类号： G01N33/53 ,  G01N21/65 ,  G01J3/44

CPC分类号： G01N21/658 ,  G01J3/44 ,  G01N21/65 ,  G01N33/54373

摘要： Raman spectra of protein immunoblots or enzyme linked immunosorbant assay procedures are acquired with a scanning Raman spectrometer. The sensitivity of the measurement is increased by conjugating secondary antibodies used in the Western blot and ELISA methods to surface enhanced Raman Scattering (SERS) labels. The resulting blot or well plate is analyzed with a Raman system that has forms a pixel map of the sample. More specifically, the Raman system generates an effectively line-shaped illumination pattern and scans the sample in the direction perpendicular to the line while the signal is accumulating on the detector. Each pixel is therefore a rectangle defined by the length of the illumination and the distance traveled by the sample within the duration of signal accumulation on the detector. The pixels are sequentially acquired to generate a map of the sample.

摘要翻译： 使用扫描拉曼光谱仪获得蛋白免疫印迹或酶联免疫吸附测定程序的拉曼光谱。 通过将Western印迹和ELISA方法中使用的二次抗体与表面增强的拉曼散射（SERS）标记缀合，来增加测量的灵敏度。 用形成样品的像素图的拉曼系统分析所得到的印迹或孔板。 更具体地，拉曼系统产生有效的线状照明图案，并且在信号积聚在检测器上的同时沿垂直于线的方向扫描样品。 因此，每个像素是由照明长度和样本在检测器上的信号累积持续时间内行进的距离所定义的矩形。 依次获取像素以生成样本的图。