公开(公告)号：US07405044B2

公开(公告)日：2008-07-29

申请号：US11245444

申请日：2005-10-07

申请人： Jerilyn A. Walker ,  Dale J. Hedges ,  Jaiprakash G. Shewale ,  Sudhir K. Sinha ,  Mark A. Batzer

发明人： Jerilyn A. Walker ,  Dale J. Hedges ,  Jaiprakash G. Shewale ,  Sudhir K. Sinha ,  Mark A. Batzer

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/6879 ,  C12Q1/6851 ,  C12Q1/6888 ,  C12Q2600/16 ,  C12Q2537/143

摘要： A comprehensive set of human specific, target specific, multiplex PCR assays for DNA quantitation is provided. Our duplex qPCR for nDNA/mtDNA had a linear quantitation range of 100 ng to 1 pg, and our triplex qPCR assay for nDNA/mtDNA/male Y DNA had a linear range of 100 ng to 0.1 ng. Human-specificity was demonstrated by the accurate detection of 0.05% and 5% human DNA, respectively, from a complex source of starting templates. Target-specificity was confirmed by the lack of cross-amplification among targets. A high throughput alternative for human gender determination was also developed by multiplexing the male Y primer/probe set with an X chromosome based system. Background cross-amplification with DNA templates derived from fourteen other species was negligible aside from the male Y assay which produced spurious amplifications from other non-human primate templates. Mainstream application of these assays will undoubtedly benefit forensic genomics.

摘要翻译： 提供了一套全面的人类特异性，靶特异性，多重PCR测定用于DNA定量。 我们用于nDNA / mtDNA的双重qPCR线性定量范围为100 ng至1 pg，我们对nDNA / mtDNA /雄性Y DNA的三重qPCR检测线性范围为100 ng至0.1 ng。 通过分别从起始模板的复杂来源准确检测0.05％和5％的人类DNA，证明了人的特异性。 目标特异性由目标缺乏交叉扩增证实。 通过将Y型引物/探针组与基于X染色体的系统进行复用也开发了用于人类性别测定的高通量替代物。 来自十四种其他物种的DNA模板的背景交叉扩增除了从其他非人灵长类动物模板产生杂种扩增的雄性Y测定之外，可忽略不计。 这些测定的主流应用无疑将有益于法医基因组学。

公开(公告)号：US07074567B2

公开(公告)日：2006-07-11

申请号：US10673854

申请日：2003-09-30

申请人： Sudhir K. Sinha ,  Dale J. Hedges ,  Mark A. Batzer

发明人： Sudhir K. Sinha ,  Dale J. Hedges ,  Mark A. Batzer

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02

CPC分类号： C12Q1/6879 ,  C12Q1/6876 ,  C12Q2600/156

摘要： A method for determining gender from a human DNA sample. The loci of Alu element insertion is selected, amplified and evaluated in terms of size of the fragment. The gender assay utilizes AluSTXa for the X chromosome, AluSTYa for the Y chromosome, or both AluSTXa and AluSTYa, to reduce the possibility of error to a negligible quantity. The inserted chromosome yields a large fragment when the homologous region is amplified. The males are distinguished as having two DNA amplicons present, while females have only a single amplicon. The kit adapted for carrying out the method includes a pair of primers to amplify the locus and optionally polymerase chain reaction regents.

公开(公告)号：US07432362B2

公开(公告)日：2008-10-07

申请号：US11431030

申请日：2006-05-10

申请人： Sudhir K. Sinha ,  Dale J. Hedges ,  Mark A. Batzer

发明人： Sudhir K. Sinha ,  Dale J. Hedges ,  Mark A. Batzer

IPC分类号： C07H21/02

CPC分类号： C12Q1/6876 ,  C12Q1/6879 ,  C12Q2600/156 ,  Y10S435/975

摘要： A method for determining gender from a human DNA sample. The loci of Alu element insertion is selected, amplified and evaluated in terms of size of the fragment. The gender assay utilizes AluSTXa for the X chromosome, AluSTYa for the Y chromosome, or both AluSTXa and AluSTYa, to reduce the possibility of error to a negligible quantity. The inserted chromosome yields a large fragment when the homologous region is amplified. The males are distinguished as having two DNA amplicons present, while females have only a single amplicon. The kit adapted for carrying out the method includes a pair of primers to amplify the locus and optionally polymerase chain reaction regents.

摘要翻译： 一种从人类DNA样本中确定性别的方法。 根据片段的大小选择，扩增和评估Alu元件插入的位点。 性别测定使用AluSTXa作为X染色体，AluSTYa用于Y染色体，或同时使用AluSTXa和AluSTYa，以将错误的可能性降低到可忽略的数量。 当扩增同源区时，插入的染色体产生大片段。 男性的区别在于存在两个DNA扩增子，而女性只有一个扩增子。 用于实施该方法的试剂盒包括一对扩增基因座和任选的聚合酶链反应试剂的引物。