公开(公告)号：US5208143A

公开(公告)日：1993-05-04

申请号：US767351

申请日：1991-09-30

申请人： Glenn L. Henderson ,  Randal A. Hoke ,  Anne C. Hopkins ,  Daniel A. McLaurin

发明人： Glenn L. Henderson ,  Randal A. Hoke ,  Anne C. Hopkins ,  Daniel A. McLaurin

IPC分类号： G01N33/543 ,  G01N33/569

CPC分类号： G01N33/56983 ,  G01N33/54393

摘要： A method for immunoassay of a viral antigen is performed on a membrane precoated with an inert protein. Nonimmunological capture of antigen takes place by absorption onto the coated membrane. Captured antigen binds to a tracer which includes a label conjugated to a specific antibody, the inert protein concomitantly inhibiting nonspecific binding of tracer. The label may be an enzyme which converts a substrate to a detectable product or converts a blocked inhibitor to an inhibitor whereby a second enzyme is inhibited from converting a substrate to a product. The invention includes a kit of materials for performing an assay in accordance with the method of the invention.

摘要翻译： 在预先涂有惰性蛋白质的膜上进行病毒抗原的免疫测定方法。 抗原的非免疫捕获通过吸收到涂覆的膜上进行。 捕获的抗原结合示踪剂，其包括与特异性抗体缀合的标记物，惰性蛋白质伴随地抑制示踪剂的非特异性结合。 标记可以是将底物转化为可检测产物或将封闭的抑制剂转化为抑制剂的酶，由此抑制第二种酶将底物转化成产物。 本发明包括用于根据本发明的方法进行测定的一组材料。

公开(公告)号：US4812293A

公开(公告)日：1989-03-14

申请号：US159485

申请日：1988-02-22

申请人： Daniel A. McLaurin ,  Beverly M. Fleming ,  James P. O'Connell

发明人： Daniel A. McLaurin ,  Beverly M. Fleming ,  James P. O'Connell

IPC分类号： B01L3/00 ,  G01N33/543 ,  G01N1/18

CPC分类号： B01L3/502 ,  G01N33/54366 ,  Y10T436/13 ,  Y10T436/255

摘要： A method for assay of an analyte and assay device therefor. The assay device includes an assay component affixed to a permeable membrane. The membrane preferably rests on a permeable membrane support and covers an opening through a receptacle for holding a liquid. The support is adjacent the open upper end of a double ended needle, the lower end of which is adapted to puncture a plug inserted in an open end of a preevacuated container. Puncturing of the plug establishes fluid communication between the prevacuated interior of the container and the opening in the receptacle whereby liquid in the receptacle is passed through the membrane. The membrane has a bubble point sufficient to prevent passage of air through the membrane after all of the liquid has been pulled through as long as the membrane remains wet so that vacuum in the tube is not lost. The device may be part of an assay workstation which comprises a canister and various utensils useful in performing the assay.

摘要翻译： 用于分析物和其测定装置的测定方法。 测定装置包括固定在可渗透膜上的测定组分。 膜优选地位于可渗透的膜支撑件上并通过用于保持液体的容器覆盖开口。 支撑件与双头针的敞开的上端相邻，其下端适于刺穿插入预先存储的容器的开口端的插头。 塞子的穿刺在容器的预浸的内部和容器中的开口之间建立流体连通，其中容器中的液体通过膜。 只要膜保持湿润，所有膜都具有足以防止空气通过膜的气泡点，以使得管中的真空不会损失。 该装置可以是测定工作站的一部分，其包括罐和可用于进行测定的各种器具。

公开(公告)号：US06235464B1

公开(公告)日：2001-05-22

申请号：US08326304

申请日：1994-10-20

申请人： Glenn L. Henderson ,  Randal A. Hoke ,  Anne C. Hopkins ,  Daniel A. McLaurin

发明人： Glenn L. Henderson ,  Randal A. Hoke ,  Anne C. Hopkins ,  Daniel A. McLaurin

IPC分类号： C12Q170

CPC分类号： G01N33/581 ,  G01N33/54393 ,  G01N33/56983 ,  Y10S435/975

摘要： A method for immunoassay of a viral antigen is performed on a membrane precoated with an inert protein. Nonimmunological capture of antigen takes place by absorption onto the coated membrane. Captured antigen binds to a tracer which includes a label conjugated to a specific antibody, the inert protein concomitantly inhibiting nonspecific binding of tracer. The label may be an enzyme which converts a substrate to a detectable product or converts a blocked inhibitor to an inhibitor whereby a second enzyme is inhibited from converting a substrate to a product. The invention includes a kit of materials for performing an assay in accordance with the method of the invention.

摘要翻译： 在预先涂有惰性蛋白质的膜上进行病毒抗原的免疫测定方法。 抗原的非免疫捕获通过吸收到涂覆的膜上进行。 捕获的抗原结合示踪剂，其包括与特异性抗体缀合的标记物，惰性蛋白质伴随地抑制示踪剂的非特异性结合。 标记可以是将底物转化为可检测产物或将封闭的抑制剂转化为抑制剂的酶，由此抑制第二种酶将底物转化成产物。 本发明包括用于根据本发明的方法进行测定的一组材料。