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1.
公开(公告)号:US20100160619A1
公开(公告)日:2010-06-24
申请号:US12718713
申请日:2010-03-05
申请人: Robert Jackson Bair , Kristen Campbell Benedict , Wendy J. Kivens , Robert W. Kwiatkowski, JR. , Kim Paulsen , Daniel A. Strom , John M. Wages
发明人: Robert Jackson Bair , Kristen Campbell Benedict , Wendy J. Kivens , Robert W. Kwiatkowski, JR. , Kim Paulsen , Daniel A. Strom , John M. Wages
IPC分类号: C07H21/00
CPC分类号: C07H21/04 , C12N15/1003 , C12N15/1006
摘要: Reagents, methods and kits for the purification of DNA from biological materials are provided.
摘要翻译: 提供了用于从生物材料中纯化DNA的试剂,方法和试剂盒。
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2.
公开(公告)号:US08598338B2
公开(公告)日:2013-12-03
申请号:US12718713
申请日:2010-03-05
申请人: Robert Jackson Bair , Kristen Campbell Benedict , Wendy J. Kivens , Robert W. Kwiatkowski, Jr. , Kim Paulsen , Daniel A. Strom , John M. Wages
发明人: Robert Jackson Bair , Kristen Campbell Benedict , Wendy J. Kivens , Robert W. Kwiatkowski, Jr. , Kim Paulsen , Daniel A. Strom , John M. Wages
IPC分类号: C07H21/04
CPC分类号: C07H21/04 , C12N15/1003 , C12N15/1006
摘要: The present disclosure provides methods for isolating substantially pure and undegraded DNA from biological material via a solid support. The methods may use a DNA lysing solution that comprises a surfactant, a DNA-complexing salt (e.g., a lithium salt), and a buffer. Alternatively, the methods may use a solid support pre-treated with the DNA lysing solution.
摘要翻译: 本公开提供了通过固体支持物从生物材料中分离基本上纯的和未降解的DNA的方法。 该方法可以使用包含表面活性剂,DNA络合盐(例如锂盐)和缓冲液的DNA裂解溶液。 或者,所述方法可以使用用DNA裂解溶液预处理的固体支持物。
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