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1.
公开(公告)号:US08598338B2
公开(公告)日:2013-12-03
申请号:US12718713
申请日:2010-03-05
申请人: Robert Jackson Bair , Kristen Campbell Benedict , Wendy J. Kivens , Robert W. Kwiatkowski, Jr. , Kim Paulsen , Daniel A. Strom , John M. Wages
发明人: Robert Jackson Bair , Kristen Campbell Benedict , Wendy J. Kivens , Robert W. Kwiatkowski, Jr. , Kim Paulsen , Daniel A. Strom , John M. Wages
IPC分类号: C07H21/04
CPC分类号: C07H21/04 , C12N15/1003 , C12N15/1006
摘要: The present disclosure provides methods for isolating substantially pure and undegraded DNA from biological material via a solid support. The methods may use a DNA lysing solution that comprises a surfactant, a DNA-complexing salt (e.g., a lithium salt), and a buffer. Alternatively, the methods may use a solid support pre-treated with the DNA lysing solution.
摘要翻译: 本公开提供了通过固体支持物从生物材料中分离基本上纯的和未降解的DNA的方法。 该方法可以使用包含表面活性剂,DNA络合盐(例如锂盐)和缓冲液的DNA裂解溶液。 或者,所述方法可以使用用DNA裂解溶液预处理的固体支持物。
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2.
公开(公告)号:US07893228B2
公开(公告)日:2011-02-22
申请号:US09974798
申请日:2001-10-12
申请人: Ellen M. Heath , John M. Wages, Jr.
发明人: Ellen M. Heath , John M. Wages, Jr.
CPC分类号: C12N15/1006
摘要: Reagents, methods and kits for the purification of RNA from biological materials are provided.
摘要翻译: 提供了用于从生物材料中纯化RNA的试剂,方法和试剂盒。
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3.
公开(公告)号:US07148343B2
公开(公告)日:2006-12-12
申请号:US10418194
申请日:2003-04-16
申请人: Robert Jackson Bair, Jr. , Ellen M. Heath , Heather Meehan , Kim Elayne Paulsen , John M. Wages, Jr.
发明人: Robert Jackson Bair, Jr. , Ellen M. Heath , Heather Meehan , Kim Elayne Paulsen , John M. Wages, Jr.
CPC分类号: C12N15/1003 , C12N15/1006
摘要: Reagents, methods and kits for the purification of RNA from biological materials are provided.
摘要翻译: 提供了用于从生物材料中纯化RNA的试剂,方法和试剂盒。
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4.
公开(公告)号:US20100160619A1
公开(公告)日:2010-06-24
申请号:US12718713
申请日:2010-03-05
申请人: Robert Jackson Bair , Kristen Campbell Benedict , Wendy J. Kivens , Robert W. Kwiatkowski, JR. , Kim Paulsen , Daniel A. Strom , John M. Wages
发明人: Robert Jackson Bair , Kristen Campbell Benedict , Wendy J. Kivens , Robert W. Kwiatkowski, JR. , Kim Paulsen , Daniel A. Strom , John M. Wages
IPC分类号: C07H21/00
CPC分类号: C07H21/04 , C12N15/1003 , C12N15/1006
摘要: Reagents, methods and kits for the purification of DNA from biological materials are provided.
摘要翻译: 提供了用于从生物材料中纯化DNA的试剂,方法和试剂盒。
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5.Reagent and method for isolation and detection of selected nucleic acid sequences 失效
标题翻译: 用于分离和检测所选择的核酸序列的试剂和方法公开(公告)号:US6060246A
公开(公告)日:2000-05-09
申请号:US969813
申请日:1997-11-13
CPC分类号: C12N15/1006
摘要: The invention relates to compositions and methods for rapidly detecting or isolating a target nucleic acid sequence in a polynucleotide-containing sample. The sample is exposed to a rapid pairing reagent, which contains a rapid capture component, effective to rapidly and non-selectively bind polynucleotides, and a target specific probe, effective to selectively bind the target nucleic acid sequence. Selectively disrupting the binding between the capture component and polynucleotides leaves only target sequence bound to the rapid pairing reagent.
摘要翻译: 本发明涉及用于在含多核苷酸的样品中快速检测或分离靶核酸序列的组合物和方法。 将样品暴露于快速配对试剂,其包含有效快速和非选择性结合多核苷酸的快速捕获组分和有效选择性结合靶核酸序列的靶特异性探针。 选择性地破坏捕获组分和多核苷酸之间的结合仅留下与快速配对试剂结合的靶序列。
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6.
公开(公告)号:US07767804B2
公开(公告)日:2010-08-03
申请号:US11589364
申请日:2006-10-30
申请人: Robert Jackson Bair, Jr. , Ellen M. Heath , Heather Meehan , Kim Elayne Paulsen , John M. Wages, Jr.
发明人: Robert Jackson Bair, Jr. , Ellen M. Heath , Heather Meehan , Kim Elayne Paulsen , John M. Wages, Jr.
CPC分类号: C12N15/1003 , C12N15/1006
摘要: Reagents, methods and kits for the purification of RNA from biological materials are provided.
摘要翻译: 提供了用于从生物材料中纯化RNA的试剂,方法和试剂盒。
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