Reagent and method for isolation and detection of selected nucleic acid
sequences
    5.
    发明授权
    Reagent and method for isolation and detection of selected nucleic acid sequences 失效
    用于分离和检测所选择的核酸序列的试剂和方法

    公开(公告)号:US6060246A

    公开(公告)日:2000-05-09

    申请号:US969813

    申请日:1997-11-13

    CPC分类号: C12N15/1006

    摘要: The invention relates to compositions and methods for rapidly detecting or isolating a target nucleic acid sequence in a polynucleotide-containing sample. The sample is exposed to a rapid pairing reagent, which contains a rapid capture component, effective to rapidly and non-selectively bind polynucleotides, and a target specific probe, effective to selectively bind the target nucleic acid sequence. Selectively disrupting the binding between the capture component and polynucleotides leaves only target sequence bound to the rapid pairing reagent.

    摘要翻译: 本发明涉及用于在含多核苷酸的样品中快速检测或分离靶核酸序列的组合物和方法。 将样品暴露于快速配对试剂,其包含有效快速和非选择性结合多核苷酸的快速捕获组分和有效选择性结合靶核酸序列的靶特异性探针。 选择性地破坏捕获组分和多核苷酸之间的结合仅留下与快速配对试剂结合的靶序列。