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1.发明授权Neutral protease (NP) and product by using neutral protease for tissue dissociation as well as method for the production thereof 有权中性蛋白酶（NP）和产品通过使用中性蛋白酶进行组织解离以及其制备方法公开(公告)号：US08440445B2
公开(公告)日：2013-05-14
申请号：US10869313
申请日：2004-06-16
申请人： Manfred Kurfürst , Christian Raemsch , Nicole Raemsch-Guenther , Olaf Friedrich , Silke Huettler , Daniel Brandhorst , Thierry Berney , Pascal Bucher , Heide Brandhorst
发明人： Manfred Kurfürst , Christian Raemsch , Nicole Raemsch-Guenther , Olaf Friedrich , Silke Huettler , Daniel Brandhorst , Thierry Berney , Pascal Bucher , Heide Brandhorst
IPC分类号： C12N9/52
CPC分类号： C12N5/0676 , C12N9/52 , C12N9/6491 , C12N2501/999 , C12N2509/00
摘要： The use of a neutral protease (NP) together with a collagenase consists in that a neutral protease which is not contained in a collagenase enzyme preparation and which is not produced by a recombinant production ia mixed before the beginning of a tissue dissociation with a collagenase or a collagenase enzyme preparation with an individual dosage of the quantitative proportions of neutral protease and collagenase for improving the isolation results with respect to yield, viability and integrity of the cells.
摘要翻译： 中性蛋白酶（NP）与胶原酶一起的使用包括：胶原酶制剂中不包含并且不是通过用胶原酶组织解离开始之前混合重组生产而产生的中性蛋白酶或 胶原酶制剂，具有定量比例的中性蛋白酶和胶原酶的单独剂量，用于改善关于细胞的产量，活力和完整性的分离结果。
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2.发明授权Method for preserving organs or limbs using a composition containing a siloxane and a semifluorinated alkane and/or hydrofluoroether 有权使用含有硅氧烷和半氟化烷烃和/或氢氟醚的组合物保存器官或肢体的方法公开(公告)号：US08029977B2
公开(公告)日：2011-10-04
申请号：US12094951
申请日：2006-11-23
IPC分类号： A01N1/00
CPC分类号： A01N1/02 , A01N1/0226
摘要： Use of a composition which contains at least one semifluorinated alkane compound and at least one liquid siloxane, wherein the composition is of a density in a range of 0.8 to 1.5 g/cm3, for preserving organs or limbs.
摘要翻译： 使用包含至少一种半氟化烷烃化合物和至少一种液体硅氧烷的组合物，其中所述组合物的密度在0.8至1.5g / cm 3范围内，用于保存器官或四肢。
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公开(公告)号：US20090226875A1
公开(公告)日：2009-09-10
申请号：US12094951
申请日：2006-11-23
IPC分类号： A01N1/02
CPC分类号： A01N1/02 , A01N1/0226
摘要： Use of a composition which contains at least one semifluorinated alkane compound and at least one liquid siloxane, wherein the composition is of a density in a range of 0.8 to 1.5 g/cm3, for preserving organs or limbs.
摘要翻译： 使用包含至少一种半氟化烷烃化合物和至少一种液体硅氧烷的组合物，其中所述组合物的密度在0.8至1.5g / cm 3范围内，用于保存器官或四肢。
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4.发明申请Neutral protease (NP) and product by using neutral protease for tissue dissociation as well as method for the production thereof 有权中性蛋白酶（NP）和产品通过使用中性蛋白酶进行组织解离以及其制备方法公开(公告)号：US20050142632A1
公开(公告)日：2005-06-30
申请号：US10869313
申请日：2004-06-16
申请人： Manfred Kurfurst , Christian Raemsch , Nicole Raemsch-Guenther , Olaf Friedrich , Silke Huettler , Daniel Brandhorst , Thierry Berney , Pascal Bucher , Heide Brandhorst
发明人： Manfred Kurfurst , Christian Raemsch , Nicole Raemsch-Guenther , Olaf Friedrich , Silke Huettler , Daniel Brandhorst , Thierry Berney , Pascal Bucher , Heide Brandhorst
CPC分类号： C12N5/0676 , C12N9/52 , C12N9/6491 , C12N2501/999 , C12N2509/00
摘要： The use of a neutral protease (NP) together with a collagenase consists in that a neutral protease which is not contained in a collagenase enzyme preparation and which is not produced by a recombinant production ia mixed before the beginning of a tissue dissociation with a collagenase or a collagenase enzyme preparation with an individual dosage of the quantitative proportions of neutral protease and collagenase for improving the isolation results with respect to yield, viability and integrity of the cells.
摘要翻译： 中性蛋白酶（NP）与胶原酶一起的使用包括：胶原酶制剂中不包含并且不是通过用胶原酶组织解离开始之前混合重组生产而产生的中性蛋白酶或 胶原酶制剂，具有定量比例的中性蛋白酶和胶原酶的单独剂量，用于改善关于细胞的产量，活力和完整性的分离结果。
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