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    PROGRAMMABLE SELF-ASSEMBLED NANOSTRUCTURES BASED ON SIDECHAIN-MODIFIED PNA FOR THE MULTIVALENT DISPLAY OF LIGANDS
    5.
    发明申请
    PROGRAMMABLE SELF-ASSEMBLED NANOSTRUCTURES BASED ON SIDECHAIN-MODIFIED PNA FOR THE MULTIVALENT DISPLAY OF LIGANDS 审中-公开
    可编程自组装的纳米结构基于改进的PNA用于多用途显示器

    公开(公告)号：US20140148500A1

    公开(公告)日：2014-05-29

    申请号：US13697123

    申请日：2011-05-11

    申请人： Daniel H. Appella

    发明人： Daniel H. Appella

    IPC分类号： A61K47/26

    CPC分类号： A61K47/26 ,  B82Y5/00 ,  C07K14/003 ,  C12N15/11 ,  C12N15/111 ,  C12N15/1138 ,  C12N2310/11 ,  C12N2310/3181 ,  C12N2310/351 ,  C12N2310/3513 ,  C12N2310/3517 ,  C12N2320/10 ,  C12N2320/30

    摘要： The invention concerns compositions comprising strands of polynucleotide and strands of PNA, each PNA strand comprising: (i) from 2 to 50 nucleobase subunits and (ii) one or more gamma substituents. The PNA strands are complementary to at least a portion of at least some of the polynucleotide strands, and the molar ratio of PNA strands to polynucleotide strands being at least 1:1. Certain gamma substituents are capable of effecting attachment of a PNA strand to a cell. The invention also concerns construction of nanostructure platforms and vaccines and use of the inventive compositions in inhibiting disease states in mammals.

    摘要翻译： 本发明涉及包含多核苷酸链和PNA链的组合物，每个PNA链包含：（i）2-50个核碱基亚基和（ii）一个或多个γ取代基。 PNA链与至少一些多核苷酸链的至少一部分互补，并且PNA链与多核苷酸链的摩尔比为至少1:1。 某些γ取代基能够使PNA链连接到细胞上。 本发明还涉及纳米结构平台和疫苗的构建以及本发明组合物用于抑制哺乳动物疾病状态的用途。

    Cross-Coupled Peptide Nucleic Acids for Detection of Nucleic Acids of Pathogens
    6.
    发明申请
    Cross-Coupled Peptide Nucleic Acids for Detection of Nucleic Acids of Pathogens 审中-公开
    用于检测病原体核酸的交叉耦联肽核酸

    公开(公告)号：US20120107794A1

    公开(公告)日：2012-05-03

    申请号：US12409159

    申请日：2009-03-23

    申请人： Daniel H. Appella ,  Christopher Micklitsch

    发明人： Daniel H. Appella ,  Christopher Micklitsch

    IPC分类号： C12Q1/70 ,  C12Q1/68

    CPC分类号： C07C271/24 ,  C07B2200/07 ,  C07C303/40 ,  C07C2601/08 ,  C07K14/003 ,  Y10T436/143333 ,  C07C311/20

    摘要： The present invention concerns methods for detecting a nucleic acid comprising (i) contacting a solution comprising a first PNA having a first cross-reactive functional group with a substrate having a second PNA affixed thereto, the second PNA having a second first cross-reactive functional group, wherein the first PNA has a reporter molecule attached thereto and the first and second PNAs being complementary to different portions of a target DNA; (ii) contacting a sample suspected of containing the nucleic acid with the first and second PNAs; and (iii) determining the presence of the reporter molecule on the substrate.

    摘要翻译： 本发明涉及用于检测核酸的方法，其包括（i）将包含具有第一交叉反应性官能团的第一PNA的溶液与其上附着有第二PNA的底物接触，所述第二PNA具有第二第一交叉反应性功能 其中所述第一PNA具有与其连接的报告分子，并且所述第一和第二PNA与靶DNA的不同部分互补; （ii）将怀疑含有核酸的样品与第一和第二PNA接触; 和（iii）确定底物上报道分子的存在。

    Cross-coupled peptide nucleic acids for detection of nucleic acids of pathogens
    8.
    发明授权
    Cross-coupled peptide nucleic acids for detection of nucleic acids of pathogens 有权
    用于检测病原体核酸的交叉偶联肽核酸

    公开(公告)号：US09156778B2

    公开(公告)日：2015-10-13

    申请号：US13592490

    申请日：2012-08-23

    申请人： Daniel H. Appella ,  Christopher Micklitsch

    发明人： Daniel H. Appella ,  Christopher Micklitsch

    IPC分类号： C12Q1/70 ,  C07C271/24 ,  C07C303/40 ,  C07K14/00

    CPC分类号： C07C271/24 ,  C07B2200/07 ,  C07C303/40 ,  C07C2601/08 ,  C07K14/003 ,  Y10T436/143333 ,  C07C311/20

    摘要： Disclosed is a method for lowering the detection limit in a method of detecting a nucleic acid comprising (i) contacting a solution comprising a first PNA with a substrate having a second PNA affixed thereto, the second PNA comprising at least one trans-cyclopentane residue, wherein the first PNA has two linker-attached biotins attached thereto and the first and second PNAs being complementary to different portions of a target DNA; (ii) contacting a sample suspected of containing the nucleic acid with the first and second PNAs; and (iii) determining the presence of the reporter molecule on the substrate. Also disclosed are a detection assay and a kit for detecting a target nucleic acid.

    摘要翻译： 本发明公开了一种降低检测核酸检测限度的方法，包括（i）使包含第一PNA的溶液与附着有第二PNA的底物接触，第二PNA包含至少一个反式环戊烷残基， 其中所述第一PNA具有与其连接的两个连接物连接的生物素，并且所述第一和第二PNA与靶DNA的不同部分互补; （ii）将怀疑含有核酸的样品与第一和第二PNA接触; 和（iii）确定底物上报道分子的存在。 还公开了检测试验和用于检测靶核酸的试剂盒。