摘要:
A gene, designated as CBF1, encoding a protein, CBF1, which binds to a region regulating expression of genes which promote cold temperature and dehydration tolerance in plants is described. CBF1 is used to transform microorganisms and can be used to transform plants.
摘要:
A method for increasing the levels of a cell protectant, such as sucrose or proline, in a cell is provided. Additionally, a method for modifying the fatty acid composition of a cell is provided. These methods may be used to enhance the cold or freezing tolerance, or drought or high salt tolerance of a cell.
摘要:
The present invention provides for a non-naturally occurring binding protein comprising an amino acid sequence capable of binding to a CCG regulatory sequence and an amino acid sequence which forms a transcription activation region. In particular the invention comprises a sequence selected from the group consisting of an AP2 domain of SEQ. I.D. Nos. 13, 15, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, and 95. When these proteins are overexpressed in a plant the plant exhibits increased tolerance to environmental stresses such as cold, freezing, drought or high salinity.
摘要翻译:本发明提供了非天然存在的结合蛋白,其包含能够结合CCG调节序列的氨基酸序列和形成转录活化区的氨基酸序列。 特别地,本发明包括选自SEQ ID NO:2的AP2结构域的序列。 ID。 第13号,第15号,第39条,第41条,第43条,第45条,第47条,第49条,第51条,第53条,第55条,第57条,第59条,第61条,第63条,第65条,第67条,第69条,第71条,第73条,第75条, 83,85,87,89,91,93和95.当这些蛋白质在植物中过表达时,植物对环境胁迫如冷,冷冻,干旱或高盐度表现出增加的耐受性。
摘要:
A transformed plant is provided which comprises one or more environmental stress tolerance genes; a DNA regulatory sequence which regulates expression of the one or more environmental stress tolerance genes; a sequence encoding a binding protein capable of binding to the DNA regulatory sequence and inducing expression of the one or more environmental stress tolerance genes; and a recombinant promoter which regulates expression of the gene encoding the binding protein. A method for altering an environmental stress tolerance of a plant is also provided which comprises the steps of transforming a plant with a promoter which regulates expression of at least one copy of a gene encoding a binding protein capable of binding to a DNA regulatory sequence which regulates one or more environmental stress tolerance genes in the plant; expressing the binding protein encoded by the gene; and stimulating expression of at least one environmental stress tolerance gene through binding of the binding protein to the DNA regulatory sequence.
摘要:
A gene, designated as CBF1, encoding a protein, CBF1, which binds to a region regulating expression of genes which promote cold temperature and dehydration tolerance in plants is described. CBF1 is used to transform microorganisms and can be used to transform plants.