公开(公告)号：US20160060663A1

公开(公告)日：2016-03-03

申请号：US14843525

申请日：2015-09-02

申请人： Katrin GRAMMANN ,  Jan Wolter ,  Liv Reinecke ,  Steffen Schaffer ,  Eileen C. Spindler ,  Wendy K. Ribble ,  Brittany L. Prather ,  Catherine B. Poor ,  Tanya Warnecke Lipscomb ,  Hans H. Liao ,  Dave A. Hogsett ,  Ronald J. Evans

发明人： Katrin GRAMMANN ,  Jan Wolter ,  Liv Reinecke ,  Steffen Schaffer ,  Eileen C. Spindler ,  Wendy K. Ribble ,  Brittany L. Prather ,  Catherine B. Poor ,  Tanya Warnecke Lipscomb ,  Hans H. Liao ,  Dave A. Hogsett ,  Ronald J. Evans

IPC分类号： C12P7/64 ,  C12N9/10

CPC分类号： C12P7/6436 ,  C12N9/1029 ,  C12Y203/01041 ,  C12Y203/01075

摘要： A microbial cell is used for producing at least one fatty acid ester, wherein the cell is genetically modified to contain (i) at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid contains at least 5 carbon atoms; and (ii) a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalyzing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester.

摘要翻译： 微生物细胞用于生产至少一种脂肪酸酯，其中所述细胞被遗传修饰以包含（i）至少一种能使细胞产生至少一种脂肪酸和/或酰基辅酶A（CoA）的第一遗传突变 ），其相对于丙二酰辅酶A依赖性和丙二酰-ACP无关脂肪酰基辅酶A代谢途径的野生型细胞增加细胞中的酶活性，其中所述脂肪酸含有至少5个碳原子; 和（ii）增加细胞中至少一种蜡酯合酶相对于野生型细胞的活性的第二种遗传突变，并且蜡酯合成酶与SEQ ID NO：1的多肽具有至少50％的序列同一性 -8及其组合或任何多肽的功能片段，用于催化脂肪酸和/或其酰基辅酶A转化为脂肪酸酯。