公开(公告)号：US6162900A

公开(公告)日：2000-12-19

申请号：US107858

申请日：1998-06-30

申请人： Mary Lou Guerinot ,  David J. Eide

发明人： Mary Lou Guerinot ,  David J. Eide

IPC分类号： A01H5/00 ,  A23L1/305 ,  A61K38/00 ,  B09C1/00 ,  B09C1/10 ,  C07K14/415 ,  C07K16/16 ,  C07K19/00 ,  C12N15/29 ,  C12N15/82 ,  G01N33/566 ,  C12N15/00

CPC分类号： B09C1/105 ,  A61K38/00 ,  C07K14/415 ,  C12N15/8242 ,  C12N15/8259 ,  C12N15/8261 ,  Y02A40/146

摘要： Isolated nucleic acid molecules encoding novel members of the MRT family of polypeptides which include, in a preferred embodiment, at least one transmembrane domain having at least about 30%, more preferably at least about 50%, 55%, 60%, 70%, 80% or 90% amino acid sequence identity with SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8 or SEQ ID NO:14 and/or at least one histidine rich domain, are described. The MRT polypeptides of the invention are capable of transporting metals such as Fe(II), Cd, Co, Mn, Pb, Hg and Zn. Transgenic plants in which expression of an MRT polypeptide of the invention is altered are also described. These transgenic plants can be used to remove pollutants from soil or as nutritional supplements to treat iron- or zinc-deficiency. Antisense nucleic acid molecules, recombinant expression vectors containing nucleic acid molecules of the invention, and host cells into which the expression vectors have been introduced are also described. The invention further provides isolated MRT polypeptides, fusion polypeptides and active fragments thereof. Therapeutic methods utilizing compositions of the invention are also provided.

摘要翻译： 编码多肽的MRT家族​​的新成员的分离的核酸分子，其在优选的实施方案中包括至少一个跨膜结构域，其具有至少约30％，更优选至少约50％，55％，60％，70％ 与SEQ ID NO：2，SEQ ID NO：4，SEQ ID NO：6，SEQ ID NO：8或SEQ ID NO：14和/或至少一个富含赖氨酸的结构域的80％或90％的氨基酸序列同一性是 描述。 本发明的MRT多肽能够输送诸如Fe（II），Cd，Co，Mn，Pb，Hg和Zn的金属。 还描述了其中本发明的MRT多肽的表达改变的转基因植物。 这些转基因植物可用于从土壤中或作为营养补充剂去除污染物以治疗铁或锌缺乏症。 还描述了反义核酸分子，含有本发明的核酸分子的重组表达载体和其中引入了表达载体的宿主细胞。 本发明还提供了分离的MRT多肽，融合多肽及其活性片段。 还提供了利用本发明组合物的治疗方法。

公开(公告)号：US5846821A

公开(公告)日：1998-12-08

申请号：US758621

申请日：1996-11-27

申请人： Mary Lou Guerinot ,  David J. Eide

发明人： Mary Lou Guerinot ,  David J. Eide

IPC分类号： A01H5/00 ,  A23L1/305 ,  A61K38/00 ,  B09C1/00 ,  B09C1/10 ,  C07K14/415 ,  C07K16/16 ,  C07K19/00 ,  C12N15/29 ,  C12N15/82 ,  G01N33/566 ,  C07H21/04

CPC分类号： B09C1/105 ,  C07K14/415 ,  C12N15/8242 ,  C12N15/8259 ,  C12N15/8261 ,  A61K38/00

摘要： Isolated nucleic acid molecules encoding novel members of the MRT family of polypeptides which include, in a preferred embodiment, at least one transmembrane domain having at least about 30%, more preferably at least about 50%, 55%, 60%, 70%, 80% or 90% amino acid sequence identity with SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8 or SEQ ID NO:14 and/or at least one histidine rich domain, are described. The MRT polypeptides of the invention are capable of transporting metals such as Fe(II), Cd, Co, Mn, Pb, Hg and Zn. Transgenic plants in which expression of an MRT polypeptide of the invention is altered are also described. These transgenic plants can be used to remove pollutants from soil or as nutritional supplements to treat iron- or zinc-deficiency. Antisense nucleic acid molecules, recombinant expression vectors containing nucleic acid molecules of the invention, and host cells into which the expression vectors have been introduced are also described. The invention further provides isolated MRT polypeptides, fusion polypeptides and active fragments thereof. Therapeutic methods utilizing compositions of the invention are also provided.

公开(公告)号：US06590140B1

公开(公告)日：2003-07-08

申请号：US09579174

申请日：2000-05-25

申请人： Mary Lou Guerinot ,  David J. Eide

发明人： Mary Lou Guerinot ,  David J. Eide

IPC分类号： A01H100

CPC分类号： B09C1/105 ,  A61K38/00 ,  C07K14/415 ,  C12N15/8242 ,  C12N15/8259 ,  C12N15/8261 ,  Y02A40/146

摘要： Isolated nucleic acid molecules encoding members of the MRT family of polypeptides which include, in a preferred embodiment, at least one transmembrane domain having at least about 30%, more preferably at least about 50%, 55%, 60%, 70%, 80% or 90% amino acid sequence identity with SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8 or SEQ ID NO:14 and/or at least one histidine rich domain, are described. The MRT polypeptides of the invention are capable of transporting metals such as Fe(II), Cd, Co, Mn, Pb, Hg and Zn. Transgenic plants in which expression of an MRT polypeptide of the invention is altered are also described. These transgenic plants can be used to remove pollutants from soil or as nutritional supplements to treat iron- or zinc-deficiency. Antisense nucleic acid molecules, recombinant expression vectors containing nucleic acid molecules of the invention, and host cells into which the expression vectors have been introduced are also described. The invention further provides isolated MRT polypeptides, fusion polypeptides and active fragments thereof. Therapeutic methods utilizing compositions of the invention are also provided.

摘要翻译： 编码多肽的MRT家族​​成员的分离的核酸分子，其在优选的实施方案中包括至少一个跨膜结构域，其具有至少约30％，更优选至少约50％，55％，60％，70％，80 描述了与SEQ ID NO：2，SEQ ID NO：4，SEQ ID NO：6，SEQ ID NO：8或SEQ ID NO：14和/或至少一个富含赖氨酸的结构域的％或90％氨基酸序列同一性 。 本发明的MRT多肽能够输送诸如Fe（II），Cd，Co，Mn，Pb，Hg和Zn的金属。 还描述了其中本发明的MRT多肽的表达改变的转基因植物。 这些转基因植物可用于从土壤中或作为营养补充剂去除污染物以治疗铁或锌缺乏症。 还描述了反义核酸分子，含有本发明的核酸分子的重组表达载体和其中引入了表达载体的宿主细胞。 本发明还提供了分离的MRT多肽，融合多肽及其活性片段。 还提供了利用本发明组合物的治疗方法。