公开(公告)号：US20240002787A1

公开(公告)日：2024-01-04

申请号：US17855141

申请日：2022-06-30

申请人： BATTELLE SAVANNAH RIVER ALLIANCE, LLC

发明人： ROBIN L. BRIGMON ,  ALEX J. KUGLER ,  CORY L. TRIVELPIECE

IPC分类号： C12N5/00 ,  B09C1/10 ,  C02F3/32 ,  C02F3/34

CPC分类号： C12N5/0068 ,  B09C1/10 ,  B09C1/105 ,  C02F3/322 ,  C02F3/347 ,  C02F2101/32 ,  C12N2533/14 ,  C12N2533/12 ,  C12N2535/00 ,  B09C2101/00 ,  C02F2305/06 ,  C02F3/343

摘要： Solid, porous, glass-based substrate for storage and delivery of useful microorganisms are described. Materials include one or more microorganisms lyophilized on the substrate, e.g., in the form of a lyophilized biofilm. The materials can be utilized for long-term storage, transport, and deployment of one or more microorganisms, such as consortium of microorganisms at a remediation site.

公开(公告)号：US20180208518A1

公开(公告)日：2018-07-26

申请号：US15878090

申请日：2018-01-23

申请人： VIRIDIS TERRA INNOVATIONS

发明人： Martin Beaudoin Nadeau ,  Donald Blouin ,  Frank Grenon

IPC分类号： C05G3/04 ,  A01G24/10 ,  A01G24/23 ,  A01G24/28 ,  A01G24/35 ,  C05F11/08 ,  C05F11/02 ,  C09K17/40 ,  C09K17/52

CPC分类号： C05G3/04 ,  A01G24/10 ,  A01G24/20 ,  A01G24/23 ,  A01G24/28 ,  A01G24/35 ,  B09C1/105 ,  C05F11/02 ,  C05F11/08 ,  C09K17/40 ,  C09K17/52

摘要： The present disclosure provides a seeding formulation for woody plants and a pre-treatment of surfaces (e.g. rocks, waste rocks, tailings, overburden) on disturbed and degraded lands. The seeding formulation includes a mix of woody trees or shrubs seeds, organic mulch, beneficial microbial populations, mineral fertilizer, adhesive, absorbent polymer, and water. The pre-treatment of surfaces refers to the spreading or mixing of surfaces with organic biomass. Methods of preparing and applying the seeding formulation and pre-treatment are also disclosed. The formulation is used for the phytoremediation, ecological restoration, land reclamation, environmental rehabilitation, revitalization, revegetation, and reforestation of disturbed and degraded lands.

公开(公告)号：US09561531B2

公开(公告)日：2017-02-07

申请号：US14624279

申请日：2015-02-17

申请人： GREEN ON INDUSTRIES, INC.

发明人： Antonio Fiorda ,  Terrence J McGuire

IPC分类号： B09C1/10 ,  C02F3/34 ,  C12N1/20 ,  C12N1/14 ,  A62D3/02 ,  C02F1/68 ,  C02F101/32 ,  E02B15/04

CPC分类号： B09C1/10 ,  A62D3/02 ,  B09C1/105 ,  C02F1/682 ,  C02F3/343 ,  C02F2101/32 ,  C02F2305/04 ,  C12N1/14 ,  C12N1/20 ,  E02B15/041 ,  Y02A20/204

摘要： The present invention is directed toward a bioremediation product, its method of manufacture and method of use. A blend of microorganisms, cleansers and surfactants is disclosed which is non-toxic and solvent free, and which has the ability to accelerate the breaking down process of oils, materials such as benzene, toluene, and xylene (BTEX); petroleum hydrocarbon fractions F1-F4 (as defined by CCME2000), polycyclic aromatic hydrocarbons (PAH), paraffin waxes, volatile organics, molds, and the like; while simultaneously providing a biological component which is effective to reduce the total petroleum hydrocarbon levels to zero at an accelerated rate. The product is useful for bioremediation and as an oil dispersant composition useful in treating oil spills, and for cleanup of the shoreline, animals, plants, and equipment.

摘要翻译： 本发明涉及生物修复产品，其制造方法和使用方法。 公开了微生物，清洁剂和表面活性剂的混合物，其是无毒和无溶剂的，并且具有加速油，诸如苯，甲苯和二甲苯（BTEX）的材料的分解过程的能力。 石油烃馏分F1-F4（由CCME2000定义），多环芳烃（PAH），石蜡，挥发性有机物，模具等; 同时提供生物组分，其有效地将总石油烃水平以加速的速率降低至零。 该产品可用于生物修复和用作处理漏油的油分散剂组合物，以及用于清理海岸线，动物，植物和设备。

公开(公告)号：US09333382B2

公开(公告)日：2016-05-10

申请号：US14251618

申请日：2014-04-13

申请人： Nankai University

发明人： Zhang Cai ,  Qixing Zhou ,  Lijuan Cheng ,  Ruiren Zhou ,  Xin Wang ,  Jingchun Tang ,  Qingmin Zhang ,  Jie Liu ,  Weitao Liu ,  Junjie Yang

IPC分类号： B09C1/10 ,  A62D3/02 ,  A62D101/20

CPC分类号： A62D3/02 ,  A62D2101/20 ,  B09C1/10 ,  B09C1/105

摘要： A plant-microorganism combined method for phhytoremediation of organic contaminated soil; i.e., an ornamental plant (Impatiens balsamina L.)—microorganisms (petroleum hydrocarbon degrading mixed microbes) combined method for enhanced phytoremediation of petroleum contaminated soil is disclosed. The inoculated petroleum degrading mixed microbes can not only degrade contaminants in soil directly but also secretes chemical substances which are helpful to relieve the toxic effects on the Impatiens balsamina L. In return, the Impatiens balsamina L. releases photosynthesis products into the soil, so as to enhance microbial activity and quantity, and assist the microorganisms in degrading target contaminates more effectively and efficiently. Repeating the processes mentioned above is able to continuously reduce petroleum hydrocarbon contaminants in the soil until the residual content of contaminants meets the environmental safety standard.

公开(公告)号：US20150273259A1

公开(公告)日：2015-10-01

申请号：US14438282

申请日：2014-03-04

申请人： UAB "BIOCENTRAS"

发明人： Saulius Grigiskis ,  Vilma Cipinyte ,  Julius Vaitoska ,  Jolante Aikaite-StanaItiene ,  Indre Gailiute ,  Grazina Rackauskiene ,  Fortunatas Grygas

IPC分类号： A62D3/02 ,  C02F3/02 ,  C02F3/32 ,  B09C1/10 ,  C02F3/34

CPC分类号： A62D3/02 ,  A62D2101/20 ,  B09C1/10 ,  B09C1/105 ,  C02F3/02 ,  C02F3/327 ,  C02F3/344 ,  C02F2101/32 ,  C02F2301/08 ,  Y02W10/15

摘要： The present invention relates to the area of environmental biotechnology. It describes environment object cleaning from oil pollutants (OPs), when they are treated with oil hydrocarbon emulsifying and oxidizing bacterial preparations and plants suitable for phytoremediation. This method is used for cleaning of soil, briny and fresh water. This invention presents a novel complex OP cleaning method, which fully or partially solves the present shortcomings with environment cleaning from OPs. Invention is different from other known oil pollutant cleaning methods, because OP cleaning is managed with a help from an expert system which comprises the evaluation of primary OP composition and environment parameters, selection of OP cleaning method and OP biodegrading microorganism blends, selection of optimal concentrations for these blends, selection of optimal OP separation and biodegradation parameters and selection of the most suitable plants for phytoremediation.

摘要翻译： 本发明涉及环境生物技术领域。 描述了油污染物（OPs）的环境对象清洗时，用油性烃类乳化和氧化细菌制剂和适合植物修复的植物进行处理。 该方法用于清洁土壤，大量和淡水。 本发明提出了一种新的复杂的OP清洗方法，其完全或部分地解决了目前OP的环境清洗的缺点。 本发明与其他已知的油污染物清洁方法不同，因为在专家系统的帮助下对OP清洁进行了管理，该专家系统包括评价主要的OP成分和环境参数，选择OP清洗方法和OP生物降解微生物混合物，选择最佳浓度 对于这些共混物，选择最佳的OP分离和生物降解参数以及选择用于植物修复的最合适的植物。

公开(公告)号：US20110052537A1

公开(公告)日：2011-03-03

申请号：US12673850

申请日：2008-08-15

申请人： A. Morrie Craig ,  Karen Walker ,  Sudeep Perumbakkam ,  Jennifer Duringer ,  Marthah Delorme

发明人： A. Morrie Craig ,  Karen Walker ,  Sudeep Perumbakkam ,  Jennifer Duringer ,  Marthah Delorme

IPC分类号： A61K35/74 ,  C12N1/20 ,  C12Q1/68 ,  A61P43/00 ,  A62D3/02

CPC分类号： B09C1/105 ,  B09C1/10 ,  F41H11/132 ,  F42B33/06

摘要： Embodiments of techniques for remediating soil contaminated with compounds, particularly nitro-based explosives, are disclosed. Plants capable of taking up the compounds are grown within contaminated soil for a period of time. The plants are exposed to anaerobic microbes in the rumen of a ruminant animal. The ruminal anaerobic microbes degrade the remediable compounds and render them substantially nontoxic to the animal. In some embodiments, the remediable compounds are nitroaromatic compounds, and are degraded by nitroreductases. In other embodiments, microbes are transferred from a consortium enriched in microbes capable of degrading the remediable compounds to a ruminant animal that lacks microbes capable of degrading the remediable compounds. In other embodiments, methods for isolating and identifying ruminal anaerobic microbes capable of degrading remediable compounds are disclosed.

摘要翻译： 公开了用于补救被化合物，特别是硝基爆炸物污染的土壤的技术的实施方案。 能够吸收化合物的植物在污染的土壤中生长一段时间。 植物暴露于反刍动物的瘤胃中的厌氧微生物。 瘤胃厌氧微生物降解可补救化合物，使其对动物基本无毒。 在一些实施方案中，可补救化合物是硝基芳族化合物，并被硝基还原酶降解。 在其他实施方案中，将微生物从富含微生物的能够将可补救化合物降解至缺乏能够降解可补救化合物的微生物的反刍动物的微生物转移。 在其它实施方案中，公开了用于分离和鉴定能够降解可治疗化合物的瘤胃厌氧微生物的方法。

公开(公告)号：US07829754B2

公开(公告)日：2010-11-09

申请号：US11662343

申请日：2005-09-09

申请人： Hideo Tamura ,  Takeshi Sato ,  Munechika Honda

发明人： Hideo Tamura ,  Takeshi Sato ,  Munechika Honda

IPC分类号： A62D3/30 ,  A62D3/33 ,  A62D3/00 ,  A62D3/02 ,  C02F3/32 ,  C02F3/34 ,  B09B3/00 ,  B09C1/10 ,  C12P1/00 ,  C12S3/00

CPC分类号： B09C1/105

摘要： A soil cleaning method (phytoremediation) of absorbing a contaminating substance contained in a contaminated soil by a plant to clean the soil. An object of the present invention is to provide a method of cleaning a contaminated soil which performs cleaning of a soil containing heavy metals in a shorter term than before and, at the same time, utilizes phytoremediation having little influence on the environment. The object is attained by absorbing heavy metals by a plant of Polygonaceae family, Fagopyrum genus, and, at the same time, adding a biodegradable chelating agent to a contaminated soil in order to assist the absorption. And, a biodegradable chelating agent having the high heavy metal dissolving out ability not only enhances the cleaning efficacy but also reduces secondary influence on the environment.

摘要翻译： 用植物吸收污染土壤中污染物质的土壤清洁方法（植物修复）来清洁土壤。 本发明的目的是提供一种清洁污染土壤的方法，所述污染土壤在比以前更短的时间内对含有重金属的土壤进行清洁，并且同时利用对环境几乎没有影响的植物修复。 该目的是通过gon科科（Fagopyrum）属的植物吸收重金属，同时在污染的土壤中加入可生物降解的螯合剂以辅助吸收。 而且，具有高重金属溶解能力的生物降解性螯合剂不仅提高了清洗效果，而且还降低了对环境的二次影响。

公开(公告)号：US20090255176A1

公开(公告)日：2009-10-15

申请号：US12310780

申请日：2006-09-07

申请人： Giusto Giovannetti

发明人： Giusto Giovannetti

IPC分类号： A01G1/00

CPC分类号： B09C1/105 ,  C12P39/00

摘要： Procedure for the decontamination of a large area of land from organic pollutants, including the phases of: a) sowing and cultivating an annual and/or perennial plant on the land to be decontaminated; b) inoculating the plant with a microbiological consortium of the rhizosphere; and c) applying to the land at least one surfactant substance, where the metabolic activity of the microbiological consortium of the rhizosphere causes the decomposition of the organic pollutants present in the soil.

摘要翻译： 包括以下各个阶段对大面积土地进行去污染的程序：a）播种和种植一年生植物和/或多年生植物，进行净化; b）用根际微生物联盟接种植物; 和c）向陆地施用至少一种表面活性剂物质，其中根际微生物联合会的代谢活性导致土壤中存在的有机污染物的分解。

公开(公告)号：US20060286658A1

公开(公告)日：2006-12-21

申请号：US11425170

申请日：2006-06-20

申请人： Justin Felder ,  Mitchell Felder

发明人： Justin Felder ,  Mitchell Felder

IPC分类号： C12N1/20 ,  C12M1/00

CPC分类号： C12N1/20 ,  B09C1/002 ,  B09C1/105 ,  C02F3/348 ,  C12M23/38

摘要： A method of growth of selected microorganism cultures by selectively baiting paraffinophilic microorganisms for concentrated growth is provided. The method includes providing a receptacle containing an aqueous solution and adjusting the solution to mimic the environmental conditions conducive to enhancing growth of the target microorganisms. The method then further includes inoculating the solution with a sample taken from an aqueous or terrestrial environmental site, or by seeding the aqueous solution with the paraffinophilic microorganism, chosen for its affinity for degrading organic waste or producing certain by-products and then placing in the receptacle one or more paraffin coated substrates to bait the paraffinophilic microorganism. An associated apparatus is also disclosed.

摘要翻译： 提供了通过选择性诱饵嗜酸性微生物浓缩生长来选择微生物培养物生长的方法。 该方法包括提供含有水溶液的容器并调节溶液以模拟有助于增强靶微生物生长的环境条件。 然后该方法还包括用从水性或陆地环境部位取出的样品接种该溶液，或者通过将该溶液与该亲水性微生物接种，选择其用于降解有机废物的亲和力或产生某些副产物，然后置于 容纳一个或多个石蜡包覆的基质以诱饵嗜盐微生物。 还公开了一种相关装置。

公开(公告)号：US07049492B1

公开(公告)日：2006-05-23

申请号：US09856561

申请日：1999-11-23

申请人： Yin-Ming Li ,  Rufus L. Chaney ,  Roger D. Reeves ,  J. Scott Angle ,  Alan J. M. Baker

发明人： Yin-Ming Li ,  Rufus L. Chaney ,  Roger D. Reeves ,  J. Scott Angle ,  Alan J. M. Baker

IPC分类号： C22B3/24 ,  A01C3/00 ,  A01C21/00

CPC分类号： B09C1/105 ,  C22B3/18 ,  C22B17/00 ,  C22B19/00 ,  Y02P10/234

摘要： A cadmium and zinc hyperaccumulating subspecies from Thlaspi caerulescense and methods for removing or recovering cadmium and zinc from soils by cultivating the Thlaspi caerulescense subspecies on soils containing cadmium and zinc.

摘要翻译： 铊和锌超累积亚种从Thlaspi caerulescense和从土壤中去除或回收镉和锌的方法，通过培养含有镉和锌的土壤上的Thlaspi caerulescense亚种。