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    Rapid screening test for smith-lemli-opitz syndrome
    1.
    发明授权
    Rapid screening test for smith-lemli-opitz syndrome 失效
    史密斯综合征快速筛查试验

    公开(公告)号：US5629210A

    公开(公告)日：1997-05-13

    申请号：US458199

    申请日：1995-06-02

    申请人： David M. Hercules ,  Edwin W. Naylor ,  Paul A. Zimmerman

    发明人： David M. Hercules ,  Edwin W. Naylor ,  Paul A. Zimmerman

    IPC分类号： G01N33/92 ,  B01D59/44

    CPC分类号： G01N33/92 ,  Y10S436/811 ,  Y10T436/24 ,  Y10T436/25375

    摘要： A rapid screening test was used to distinguish between blood of normal infants and that of individuals having the Smith-Lemli-Opitz Syndrome (SLO). Blood was spotted on filter paper and analyzed with no extractions or separations. The method comprises the steps of vaporizing a cholesterol containing organic sample, ionizing the vaporized sample, detecting fragment ions of said vaporized ionized sample, and determining the intensity ratio of said fragment ions for cholesterol/7-dehydrocholesterol in the sample. The method includes performing the analysis by time-of-flight secondary ion mass spectrometry without derivation or chemical steps.

    摘要翻译： 使用快速筛选试验来区分正常婴儿的血液和具有史密斯 - 莱米 - 奥itz综合征（SLO）的患者的血液。 在滤纸上发现血液，并进行分析，无需提取或分离。 该方法包括蒸发含胆固醇的有机样品，电离蒸发的样品，检测所述蒸发的电离样品的碎片离子，以及确定样品中所述碎片离子对胆固醇/ 7-脱氢胆固醇的强度比。 该方法包括通过飞行时间次级离子质谱法进行分析，而无需推导或化学步骤。

    System and method for on-line coupling of liquid capillary separations with matrix-assisted laser desorption/ionization mass spectrometry
    2.
    发明授权
    System and method for on-line coupling of liquid capillary separations with matrix-assisted laser desorption/ionization mass spectrometry 失效
    液相毛细管分离的在线耦合与基质辅助激光解吸/电离质谱法的系统和方法

    公开(公告)号：US6140639A

    公开(公告)日：2000-10-31

    申请号：US86894

    申请日：1998-05-29

    申请人： Arkady I. Gusev ,  David M. Hercules

    发明人： Arkady I. Gusev ,  David M. Hercules

    IPC分类号： H01J49/04 ,  H01J49/16

    CPC分类号： H01J49/164 ,  H01J49/0431

    摘要： The invention relates to a system and method for on-line coupling of liquid capillary separation with matrix-assisted laser desorption ionization mass spectrometric analysis. In this system and method, analyte from liquid capillary separation is mixed with matrix molecules for matrix-assisted laser desorption ionization. Continuous flow of the analyte/matrix combined with vacuum conditions allows evaporation and crystallization of homogeneous samples on a solid sample surface. Dual use of laser irradiation to desorb/ionize and remove excess sample facilitates on-line use and automation.

    摘要翻译： 本发明涉及一种用于在线耦合液体毛细管分离与基质辅助激光解吸电离质谱分析的系统和方法。 在该系统和方法中，将来自液体毛细管分离的分析物与用于基质辅助激光解吸电离的基质分子混合。 分析物/基质的连续流动结合真空条件允许均匀样品在固体样品表面上的蒸发和结晶。 双重使用激光照射解吸/电离和去除多余的样品有助于在线使用和自动化。