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公开(公告)号：US09068997B2
公开(公告)日：2015-06-30
申请号：US13878820
申请日：2011-10-11
CPC分类号： G01N33/948 , G01N30/06 , G01N30/24 , G01N2030/067 , G01N2030/085 , Y10T436/11 , Y10T436/203332 , Y10T436/255
摘要： Disclosed is an apparatus for automatically extracting i2,6-diisopropylphenol (Propofol), from a complex sample matrix comprising cellular material, e.g. blood, the apparatus comprising a sample reception stage having on output for providing a defined quantity of the complex sample matrix; a mixing stage having a first input in fluidic connection with the output of the sample reception stage, a second input for receiving a lysing agent and an output for providing a mixture of the defined quantity of the complex sample matrix and the lysing agent; a delay stage having an input in fluidic connection with the output of the mixing stage and an output for providing the delayed mixture of the defined quantity of the complex sample matrix and the lysing agent; a filtering stage comprising a sorbent material for mechanically lysing the cellular material, said sorbent material having an affinity for binding Propofol, said filtering stage having an input in fluidic connection with the output of the delay stage; and a controller for controlling the flow rate of the mixture of the defined quantity of the complex sample matrix and the lysing agent through said delay stage. A method for such extraction is also disclosed.
摘要翻译： 公开了一种用于从包含细胞材料的复杂样品基质中自动提取i2,6-二异丙基苯酚（异丙酚）的装置，例如， 血液，所述装置包括具有用于提供限定量的所述复样本矩阵的输出的样本接收台; 混合阶段具有与样品接收台的输出流体连接的第一输入，用于接收裂解剂的第二输入和用于提供所定量的复合样品基质和裂解剂的混合物的输出; 具有与混合级的输出流体连接的输入的延迟级和用于提供限定量的复合样品基质和裂解剂的延迟混合物的输出; 过滤阶段包括用于机械裂解细胞材料的吸附剂材料，所述吸附剂材料具有对结合丙泊酚的亲和力，所述过滤级具有与延迟级的输出流体连接的输入; 以及控制器，用于通过所述延迟级来控制所述限定量的所述复合样本矩阵和所述裂解剂的混合物的流量。 还公开了这种提取的方法。
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公开(公告)号：US09068996B2
公开(公告)日：2015-06-30
申请号：US13878815
申请日：2011-10-11
IPC分类号： G01N33/94 , G01N33/50 , G01N33/52 , G01N33/557
CPC分类号： G01N33/948 , G01N33/52 , G01N33/557 , Y10T436/14 , Y10T436/203332
摘要： Disclosed is a method of determining the presence of an analyte of interest by means of a detection of a reaction product between the analyte of interest and a reactant, the method comprising extracting the analyte of interest from a complex sample matrix; transferring the analyte of interest to an initial reaction mixture; performing a background measurement on the initial reaction mixture comprising at most a negligible concentration of the reaction product, wherein the reaction conditions present in said initial reaction mixture at least reduce the reaction rate of the formation of the reaction product such that the background measurement can be performed without a measurable change in said negligible concentration; altering the reaction conditions in the initial reaction mixture to accelerate said reaction rate; continuing said reaction until the concentration of the reaction product has stabilized; performing a second measurement on the resultant reaction mixture to obtain a signal correlated to said concentration; and determining the presence of the analyte of interest from a difference between the background measurement and the second measurement. In a preferred embodiment, the analyte of interest is Propofol (2,6-di-isopropylphenol), and the reactant is the activated Gibbs reagent (2,6-dichloroquinoneimine).
摘要翻译： 公开了通过检测目标分析物和反应物之间的反应产物来确定感兴趣分析物的存在的方法，该方法包括从复杂样品基质提取目的分析物; 将目标分析物转移到初始反应混合物中; 对最初反应混合物进行背景测量，该反应混合物至少含有可忽略的反应产物浓度，其中存在于所述初始反应混合物中的反应条件至少降低反应产物形成的反应速率，使得背景测量可以 在所述可忽略的浓度中没有可测量的变化; 改变初始反应混合物中的反应条件以加速所述反应速率; 继续反应直到反应产物的浓度稳定; 对所得反应混合物进行第二次测定以获得与所述浓度相关的信号; 以及从背景测量和第二测量之间的差异确定感兴趣分析物的存在。 在优选的实施方案中，感兴趣的分析物是异丙酚（2,6-二异丙基苯酚），反应物是活化的吉布斯试剂（2,6-二氯喹啉亚胺）。
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公开(公告)号：US20130316464A1
公开(公告)日：2013-11-28
申请号：US13878820
申请日：2011-10-11
IPC分类号： G01N33/94
CPC分类号： G01N33/948 , G01N30/06 , G01N30/24 , G01N2030/067 , G01N2030/085 , Y10T436/11 , Y10T436/203332 , Y10T436/255
摘要： Disclosed is an apparatus for automatically extracting i2,6-diisopropylphenol (Propofol), from a complex sample matrix comprising cellular material, e.g. blood, the apparatus comprising a sample reception stage having on output for providing a defined quantity of the complex sample matrix; a mixing stage having a first input in fluidic connection with the output of the sample reception stage, a second input for receiving a lysing agent and an output for providing a mixture of the defined quantity of the complex sample matrix and the lysing agent; a delay stage having an input in fluidic connection with the output of the mixing stage and an output for providing the delayed mixture of the defined quantity of the complex sample matrix and the lysing agent; a filtering stage comprising a sorbent material for mechanically lysing the cellular material, said sorbent material having an affinity for binding Propofol, said filtering stage having an input in fluidic connection with the output of the delay stage; and a controller for controlling the flow rate of the mixture of the defined quantity of the complex sample matrix and the lysing agent through said delay stage. A method for such extraction is also disclosed.
摘要翻译： 公开了一种用于从包含细胞材料的复杂样品基质中自动提取i2,6-二异丙基苯酚（异丙酚）的装置，例如， 血液，所述装置包括具有用于提供限定量的所述复样本矩阵的输出的样本接收台; 混合阶段具有与样品接收台的输出流体连接的第一输入，用于接收裂解剂的第二输入和用于提供所定量的复合样品基质和裂解剂的混合物的输出; 具有与混合级的输出流体连接的输入的延迟级和用于提供限定量的复合样品基质和裂解剂的延迟混合物的输出; 过滤阶段包括用于机械裂解细胞材料的吸附剂材料，所述吸附剂材料具有对结合丙泊酚的亲和力，所述过滤级具有与延迟级的输出流体连接的输入; 以及控制器，用于通过所述延迟级来控制所述限定量的所述复合样本矩阵和所述裂解剂的混合物的流量。 还公开了这种提取的方法。
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公开(公告)号：US20130302781A1
公开(公告)日：2013-11-14
申请号：US13878815
申请日：2011-10-11
IPC分类号： G01N33/94
CPC分类号： G01N33/948 , G01N33/52 , G01N33/557 , Y10T436/14 , Y10T436/203332
摘要： Disclosed is a method of determining the presence of an analyte of interest by means of a detection of a reaction product between the analyte of interest and a reactant, the method comprising extracting the analyte of interest from a complex sample matrix; transferring the analyte of interest to an initial reaction mixture; performing a background measurement on the initial reaction mixture comprising at most a negligible concentration of the reaction product, wherein the reaction conditions present in said initial reaction mixture at least reduce the reaction rate of the formation of the reaction product such that the background measurement can be performed without a measurable change in said negligible concentration; altering the reaction conditions in the initial reaction mixture to accelerate said reaction rate; continuing said reaction until the concentration of the reaction product has stabilized; performing a second measurement on the resultant reaction mixture to obtain a signal correlated to said concentration; and determining the presence of the analyte of interest from a difference between the background measurement and the second measurement. In a preferred embodiment, the analyte of interest is Propofol (2,6-di-isopropylphenol), and the reactant is the activated Gibbs reagent (2,6-dichloroquinoneimine).
摘要翻译： 公开了通过检测目标分析物和反应物之间的反应产物来确定感兴趣分析物的存在的方法，该方法包括从复杂样品基质提取目的分析物; 将目标分析物转移到初始反应混合物中; 对最初反应混合物进行背景测量，该反应混合物至少含有可忽略的反应产物浓度，其中存在于所述初始反应混合物中的反应条件至少降低反应产物形成的反应速率，使得背景测量可以 在所述可忽略的浓度中没有可测量的变化; 改变初始反应混合物中的反应条件以加速所述反应速率; 继续反应直到反应产物的浓度稳定; 对所得反应混合物进行第二次测定以获得与所述浓度相关的信号; 以及从背景测量和第二测量之间的差异确定感兴趣分析物的存在。 在优选的实施方案中，感兴趣的分析物是异丙酚（2,6-二异丙基苯酚），反应物是活化的吉布斯试剂（2,6-二氯喹啉亚胺）。
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