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    SWELLABLE (METH)ACRYLATE SURFACES FOR CULTURING CELLS IN CHEMICALLY DEFINED MEDIA
    1.
    发明申请
    SWELLABLE (METH)ACRYLATE SURFACES FOR CULTURING CELLS IN CHEMICALLY DEFINED MEDIA 有权
    用于化学定义培养基培养细胞的（甲基）丙烯酸酯表面

    公开(公告)号：US20090191632A1

    公开(公告)日：2009-07-30

    申请号：US12362974

    申请日：2009-01-30

    申请人： Andrei G. Fadeev ,  Jennifer Gehman ,  Arthur Winston Martin ,  Zara Melkoumian ,  Christopher B. Shogbon ,  David Michael Weber ,  Yue Zhou

    发明人： Andrei G. Fadeev ,  Jennifer Gehman ,  Arthur Winston Martin ,  Zara Melkoumian ,  Christopher B. Shogbon ,  David Michael Weber ,  Yue Zhou

    IPC分类号： C12N5/02

    CPC分类号： C12N5/0606 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2533/50

    摘要： Synthetic surfaces capable of supporting culture of undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a peptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes hydroxyethyl methacrylate, 2-carboxyehylacrylate, and tetra(ethylene glycol) dimethacrylate. The conjugated peptide may include an amino acid sequence of XaanProGlnValThrArgGlyAspValPheThrMetPro, where n is an integer from 0 to 3 and where Xaa is any amino acid. Further, disclosed herein is a swellable (meth)acrylate synthetic surface which can be sterilized by gamma irradiation.

    摘要翻译： 能够支持未分化的人类胚胎干细胞培养在化学成分确定的培养基中的合成表面包括可溶胀的（甲基）丙烯酸酯层和与可溶胀（甲基）丙烯酸酯层共轭的肽。 可溶胀的（甲基）丙烯酸酯层可以通过使包含甲基丙烯酸羟乙酯，2-羧基丙烯酸烯丙酯和四（乙二醇）二甲基丙烯酸酯的组合物中的单体聚合而形成。 缀合肽可以包括XananProGlnValThrArgGlyAspValPheThrMetPro的氨基酸序列，其中n是0至3的整数，并且其中Xaa是任何氨基酸。 此外，本文公开了可通过γ辐射灭菌的可溶胀（甲基）丙烯酸酯合成表面。

    Synthetic surfaces for culturing cells in chemically defined media
    2.
    发明授权
    Synthetic surfaces for culturing cells in chemically defined media 有权
    合成表面，用于在化学成分确定的培养基中培养细胞

    公开(公告)号：US08354274B2

    公开(公告)日：2013-01-15

    申请号：US12362924

    申请日：2009-01-30

    申请人： Andrei Gennadyevich Fadeev ,  Jennifer Gehman ,  Zara Melkoumian ,  David Michael Weber ,  Yue Zhou

    发明人： Andrei Gennadyevich Fadeev ,  Jennifer Gehman ,  Zara Melkoumian ,  David Michael Weber ,  Yue Zhou

    IPC分类号： C12N5/071 ,  C12N11/00 ,  C12N11/08

    CPC分类号： C12N5/0068 ,  C12N5/0606 ,  C12N2533/30 ,  C12N2533/50 ,  C12N2537/10

    摘要： Synthetic surfaces capable of supporting culture of eukaryotic cells including stem cells and undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a polypeptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes a carboxyl group-containing (meth)acrylate monomer, a cross-linking (di- or higher-functional) (meth)acrylate monomer, and a hydrophilic monomer capable of polymerizing with the carboxyl group-containing (meth)acrylate monomer and the cross-linking (meth)acrylate monomer. The swellable (meth)acrylate layer has an equilibrium water content in water of between about 5% and about 70%. The conjugated peptide may include an RGD amino acid sequence.

    摘要翻译： 能够支持培养真核细胞（包括干细胞和未分化的人类胚胎干细胞）的合成表面包括可溶胀的（甲基）丙烯酸酯层和与可溶胀的（甲基）丙烯酸酯层共轭的多肽。 可溶胀的（甲基）丙烯酸酯层可以通过将包含含羧基的（甲基）丙烯酸酯单体，交联（二官能或更高官能）（甲基）丙烯酸酯单体和亲水性（甲基）丙烯酸酯单体）的组合物中的单体聚合而形成 能够与含羧基（甲基）丙烯酸酯单体和交联（甲基）丙烯酸酯单体聚合的单体。 可溶胀的（甲基）丙烯酸酯层在水中的平衡水含量为约5％至约70％。 缀合的肽可以包括RGD氨基酸序列。

    Synthetic Surfaces for Differentiating Stem Cells into Cardiomyocytes
    5.
    发明申请
    Synthetic Surfaces for Differentiating Stem Cells into Cardiomyocytes 审中-公开
    用于将干细胞分化成心肌细胞的合成表面

    公开(公告)号：US20100248366A1

    公开(公告)日：2010-09-30

    申请号：US12701731

    申请日：2010-02-08

    申请人： Andrei Gennadyevich Fadeev ,  Jennifer Weber ,  Zara Melkoumian ,  David Michael Weber ,  Yue Zhou ,  Ralph Brandenberger

    发明人： Andrei Gennadyevich Fadeev ,  Jennifer Weber ,  Zara Melkoumian ,  David Michael Weber ,  Yue Zhou ,  Ralph Brandenberger

    IPC分类号： C12N5/0735

    CPC分类号： C12N5/0068 ,  C12N5/0606 ,  C12N2533/30 ,  C12N2533/50 ,  C12N2537/10

    摘要： Synthetic surfaces capable of supporting culture of eukaryotic cells including stem cells and undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a polypeptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes a carboxyl group-containing (meth)acrylate monomer, a cross-linking (di- or higher-functional) (meth)acrylate monomer, and a hydrophilic monomer capable of polymerizing with the carboxyl group-containing (meth)acrylate monomer and the cross-linking (meth)acrylate monomer. The swellable (meth)acrylate layer has an equilibrium water content in water of between about 5% and about 70%. The conjugated peptide may include an RGD amino acid sequence.

    摘要翻译： 能够支持培养真核细胞（包括干细胞和未分化的人类胚胎干细胞）的合成表面包括可溶胀的（甲基）丙烯酸酯层和与可溶胀的（甲基）丙烯酸酯层共轭的多肽。 可溶胀的（甲基）丙烯酸酯层可以通过将包含含羧基的（甲基）丙烯酸酯单体，交联（二官能或更高官能）（甲基）丙烯酸酯单体和亲水性（甲基）丙烯酸酯单体）的组合物中的单体聚合而形成 能够与含羧基（甲基）丙烯酸酯单体和交联（甲基）丙烯酸酯单体聚合的单体。 可溶胀的（甲基）丙烯酸酯层在水中的平衡水含量为约5％至约70％。 缀合的肽可以包括RGD氨基酸序列。

    SYNTHETIC SURFACES FOR CULTURING CELLS IN CHEMICALLY DEFINED MEDIA
    6.
    发明申请
    SYNTHETIC SURFACES FOR CULTURING CELLS IN CHEMICALLY DEFINED MEDIA 有权
    用于培养细胞的合成表面在化学定义的介质中

    公开(公告)号：US20090191627A1

    公开(公告)日：2009-07-30

    申请号：US12362924

    申请日：2009-01-30

    申请人： Andrei Gennadyevich Fadeev ,  Jennifer Gehman ,  Zara Melkoumian ,  David Michael Weber ,  Yue Zhou

    发明人： Andrei Gennadyevich Fadeev ,  Jennifer Gehman ,  Zara Melkoumian ,  David Michael Weber ,  Yue Zhou

    IPC分类号： C12N5/08 ,  C12N5/06

    CPC分类号： C12N5/0068 ,  C12N5/0606 ,  C12N2533/30 ,  C12N2533/50 ,  C12N2537/10

    摘要： Synthetic surfaces capable of supporting culture of eukaryotic cells including stem cells and undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a polypeptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes a carboxyl group-containing (meth)acrylate monomer, a cross-linking (di- or higher-functional) (meth)acrylate monomer, and a hydrophilic monomer capable of polymerizing with the carboxyl group-containing (meth)acrylate monomer and the cross-linking (meth)acrylate monomer. The swellable (meth)acrylate layer has an equilibrium water content in water of between about 5% and about 70%. The conjugated peptide may include an RGD amino acid sequence.

    摘要翻译： 能够支持培养真核细胞（包括干细胞和未分化的人类胚胎干细胞）的合成表面包括可溶胀的（甲基）丙烯酸酯层和与可溶胀的（甲基）丙烯酸酯层共轭的多肽。 可溶胀的（甲基）丙烯酸酯层可以通过将包含含羧基的（甲基）丙烯酸酯单体，交联（二官能或更高官能）（甲基）丙烯酸酯单体和亲水性（甲基）丙烯酸酯单体）的组合物中的单体聚合而形成 能够与含羧基（甲基）丙烯酸酯单体和交联（甲基）丙烯酸酯单体聚合的单体。 可溶胀的（甲基）丙烯酸酯层在水中的平衡水含量为约5％至约70％。 缀合的肽可以包括RGD氨基酸序列。

    REACTIVE SURFACE ON A POLYMERIC SUBSTRATE
    9.
    发明申请
    REACTIVE SURFACE ON A POLYMERIC SUBSTRATE 审中-公开
    聚合物基体上的反应性表面

    公开(公告)号：US20090081797A1

    公开(公告)日：2009-03-26

    申请号：US12201029

    申请日：2008-08-29

    申请人： ANDREI G FADEEV ,  Santona Pal ,  David Michael Weber

    发明人： ANDREI G FADEEV ,  Santona Pal ,  David Michael Weber

    IPC分类号： G01N33/68 ,  C08J3/28 ,  C08F8/32

    CPC分类号： C08J7/123 ,  C08J2365/00

    摘要： Plasma treated cyclic polyolefin copolymer surfaces having enhanced binding density for binding biologically active agents and cells are provided. These plasma treated cyclic polyolefin copolymer surfaces may be further enhanced for binding biologically active agents or cells by the application of conjugates. Methods of making and characterizing treated polymer surfaces are also provided.

    摘要翻译： 提供了具有增强的结合生物活性剂和细胞结合密度的等离子体处理的环状聚烯烃共聚物表面。 这些等离子体处理的环状聚烯烃共聚物表面可以进一步增强，以通过施用共轭物结合生物活性剂或细胞。 还提供了制备和表征经处理的聚合物表面的方法。

    Swellable (meth)acrylate surfaces for culturing cells in chemically defined media
    10.
    发明授权
    Swellable (meth)acrylate surfaces for culturing cells in chemically defined media 有权
    可溶胀的（甲基）丙烯酸酯表面用于在化学上限定的培养基中培养细胞

    公开(公告)号：US08329469B2

    公开(公告)日：2012-12-11

    申请号：US12362974

    申请日：2009-01-30

    申请人： Andrei G. Fadeev ,  Jennifer Gehman ,  Arthur Winston Martin ,  Zara Melkoumian ,  Christopher B. Shogbon ,  David Michael Weber ,  Yue Zhou

    发明人： Andrei G. Fadeev ,  Jennifer Gehman ,  Arthur Winston Martin ,  Zara Melkoumian ,  Christopher B. Shogbon ,  David Michael Weber ,  Yue Zhou

    IPC分类号： C12N5/00 ,  C12N11/00

    CPC分类号： C12N5/0606 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2533/50

    摘要： Synthetic surfaces capable of supporting culture of undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a peptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes hydroxyethyl methacrylate, 2-carboxyehylacrylate, and tetra(ethylene glycol) dimethacrylate. The conjugated peptide may include an amino acid sequence of XaanProGlnValThrArgGlyAspValPheThrMetPro, where n is an integer from 0 to 3 and where Xaa is any amino acid. Further, disclosed herein is a swellable (meth)acrylate synthetic surface which can be sterilized by gamma irradiation.

    摘要翻译： 能够支持未分化的人类胚胎干细胞培养在化学成分确定的培养基中的合成表面包括可溶胀的（甲基）丙烯酸酯层和与可溶胀的（甲基）丙烯酸酯层共轭的肽。 可溶胀的（甲基）丙烯酸酯层可以通过使包含甲基丙烯酸羟乙酯，2-羧基丙烯酸烯丙酯和四（乙二醇）二甲基丙烯酸酯的组合物中的单体聚合而形成。 缀合肽可以包括XananProGlnValThrArgGlyAspValPheThrMetPro的氨基酸序列，其中n是0至3的整数，并且其中Xaa是任何氨基酸。 此外，本文公开了可通过γ辐射灭菌的可溶胀（甲基）丙烯酸酯合成表面。