Paired end sequencing
    1.
    发明申请
    Paired end sequencing 审中-公开
    配对测序

    公开(公告)号:US20090233291A1

    公开(公告)日:2009-09-17

    申请号:US12322119

    申请日:2009-01-28

    IPC分类号: C12Q1/68

    摘要: An embodiment of a method for obtaining a DNA construct comprising two end regions of a target nucleic acid in an in vitro reaction is described that comprises the steps of: fragmenting a large nucleic acid molecule to produce a target nucleic acid molecule; ligating a recombination adaptor element to each end of the target nucleic acid molecule to produce an adapted target nucleic acid molecule; exposing the adapted target nucleic acid to a site specific recombinase to produce a circular nucleic acid product and a linear nucleic acid product from the adapted target nucleic acid, wherein the circular nucleic acid product comprises the target nucleic acid molecule; and fragmenting the circular nucleic acid product to produce a template nucleic acid molecule comprising a sequence region from each end of the target nucleic acid molecule.

    摘要翻译: 描述了在体外反应中获得包含靶核酸的两个末端区域的DNA构建体的方法的实施方案,其包括以下步骤:将大核酸分子片段化以产生靶核酸分子; 将重组衔接子元件连接到靶核酸分子的每个末端以产生适应的靶核酸分子; 将适配的靶核酸暴露于位点特异性重组酶以产生来自适应的靶核酸的环状核酸产物和线性核酸产物,其中所述环状核酸产物包含靶核酸分子; 并将环状核酸产物片段化以产生包含靶核酸分子每端的序列区的模板核酸分子。