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公开(公告)号：US20100222232A1
公开(公告)日：2010-09-02
申请号：US12754308
申请日：2010-04-05
申请人： Thomas J. Albert , Roland Green , Todd Richmond , Michael Molla , Jeffrey Jeddeloh , Jason Patrick Affourtit , Mathreyan Srinivasan , Brian Christopher Godwin , Matthew Rodesch
发明人： Thomas J. Albert , Roland Green , Todd Richmond , Michael Molla , Jeffrey Jeddeloh , Jason Patrick Affourtit , Mathreyan Srinivasan , Brian Christopher Godwin , Matthew Rodesch
CPC分类号： C12N15/1093 , C12Q1/6834
摘要： The present invention provides novel methods for reducing the complexity of preferably a genomic sample for further analysis such as direct DNA sequencing, resequencing or SNP calling. The methods use pre-selected immobilized oligonucleotide probes to capture target nucleic acid molecules from a sample containing denatured, fragmented (genomic) nucleic acids for reducing the genetic complexity of the original population of nucleic acid molecules.
摘要翻译： 本发明提供了用于降低优选用于进一步分析的基因组样品的复杂性的新方法，例如直接DNA测序，再测序或SNP呼叫。 该方法使用预先选择的固定化寡核苷酸探针从含有变性，片段（基因组）核酸的样品中捕获靶核酸分子，以降低核酸分子的原始群体的遗传复杂性。
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公开(公告)号：US20110184161A1
公开(公告)日：2011-07-28
申请号：US13084259
申请日：2011-04-11
申请人： THOMAS J. ALBERT
发明人： THOMAS J. ALBERT
IPC分类号： C07H21/04
CPC分类号： C12N15/1093 , C12Q1/6806 , C12Q2565/501
摘要： The present invention provides novel methods for reducing the complexity of a genomic sample for further analysis such as direct DNA sequencing, resequencing or SNP calling. The methods use pre-selected immobilized oligonucleotide probes to capture target nucleic acid molecules from a sample containing denatured, fragmented genomic nucleic acid. The disclosed method provides for cost-effective, flexible and rapid enrichment of target nucleic acid from complex biological samples.
摘要翻译： 本发明提供了用于降低用于进一步分析的基因组样品的复杂性的新方法，例如直接DNA测序，再测序或SNP呼叫。 该方法使用预选的固定化寡核苷酸探针从含有变性，片段化的基因组核酸的样品中捕获靶核酸分子。 所公开的方法提供来自复杂生物样品的靶核酸的成本效益，灵活和快速富集。
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公开(公告)号：US20080194414A1
公开(公告)日：2008-08-14
申请号：US11970949
申请日：2008-01-08
申请人： Thomas J. Albert , Roland Green , Todd Richmond , Michael Molla , Jeffrey Jeddeloh , Jason Patrick Affourtit , Mathreyan Srinivasan , Brian Christopher Godwin , Matthew Rodesch
发明人： Thomas J. Albert , Roland Green , Todd Richmond , Michael Molla , Jeffrey Jeddeloh , Jason Patrick Affourtit , Mathreyan Srinivasan , Brian Christopher Godwin , Matthew Rodesch
CPC分类号： C12N15/1093 , C12Q1/6834
摘要： The present invention provides novel methods for reducing the complexity of preferably a genomic sample for further analysis such as direct DNA sequencing, resequencing or SNP calling. The methods use pre-selected immobilized oligonucleotide probes to capture target nucleic acid molecules from a sample containing denatured, fragmented (genomic) nucleic acids for reducing the genetic complexity of the original population of nucleic acid molecules.
摘要翻译： 本发明提供了用于降低优选用于进一步分析的基因组样品的复杂性的新方法，例如直接DNA测序，再测序或SNP呼叫。 该方法使用预先选择的固定化寡核苷酸探针从含有变性，片段（基因组）核酸的样品中捕获靶核酸分子，以降低核酸分子的原始群体的遗传复杂性。
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4.发明申请System and method for improved processing of nucleic acids for production of sequencable libraries 审中-公开用于生产可序列文库的核酸改进处理系统和方法公开(公告)号：US20110003701A1
公开(公告)日：2011-01-06
申请号：US12380139
申请日：2009-02-23
申请人： Gianni Calogero Ferreri , Jan F. Simons , Michael Todd Ronan , Michael Egholm , Brian C. Godwin , David Roderick Riches , Stephen Kyle Hutchison , Michael S. Braverman , Melinda D. Palmer , Jeffrey Jeddeloh , Jacob Kitzman , Thomas J. Albert
发明人： Gianni Calogero Ferreri , Jan F. Simons , Michael Todd Ronan , Michael Egholm , Brian C. Godwin , David Roderick Riches , Stephen Kyle Hutchison , Michael S. Braverman , Melinda D. Palmer , Jeffrey Jeddeloh , Jacob Kitzman , Thomas J. Albert
CPC分类号： C12N15/66
摘要： An embodiment of an adaptor element for efficient target processing is described that comprises a semi-complementary double stranded nucleic acid adaptor comprising a non-complementary region and a complementary region, where the non-complementary region comprises a first amplification primer site and a second amplification primer site and the complementary region comprises a sequencing primer site and one or more inosine species.
摘要翻译： 描述了用于有效靶处理的衔接子元件的实施方案，其包含半互补双链核酸衔接子，其包含非互补区域和互补区域，其中所述非互补区域包含第一扩增引物位点和第二扩增 引物位点和互补区包含测序引物位点和一种或多种肌苷物质。
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公开(公告)号：US07892732B2
公开(公告)日：2011-02-22
申请号：US11034374
申请日：2005-01-12
申请人： Roland Green , Thomas J. Albert
发明人： Roland Green , Thomas J. Albert
CPC分类号： C12Q1/6837 , C12Q1/686 , C12Q2565/543 , C12Q2525/155 , C12Q2565/501 , C12Q2537/143
摘要： The present invention provides a method of amplifying target DNA by PCR or multiplex PCR on a microarray using array-immobilized DNA probes synthesized in a common area on the microarray by a maskless array synthesizer (MAS). The MAS constructed array-immobilized DNA probes include a universal primer linked to a sequence-specific probe, and optionally a calibrated probe for use in quantifying amplified target DNA.
摘要翻译： 本发明提供一种通过PCR或多重PCR在微阵列上使用通过无掩模阵列合成仪（MAS）在微阵列上的公共区域中合成的阵列固定的DNA探针扩增靶DNA的方法。 MAS构建的阵列固定的DNA探针包括与序列特异性探针连接的通用引物，以及可选地用于定量扩增的靶DNA的校准探针。
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公开(公告)号：US20080194413A1
公开(公告)日：2008-08-14
申请号：US11789135
申请日：2007-04-24
申请人： Thomas J. Albert
发明人： Thomas J. Albert
IPC分类号： C40B10/00
CPC分类号： C12N15/1093 , C12Q1/6806 , C12Q2565/501
摘要： The present invention provides novel methods for reducing the complexity of a genomic sample for further analysis such as direct DNA sequencing, resequencing or SNP calling. The methods use pre-selected immobilized oligonucleotide probes to capture target nucleic acid molecules from a sample containing denatured, fragmented genomic nucleic acid. The disclosed method provides for cost-effective, flexible and rapid enrichment of target nucleic acid from complex biological samples.
摘要翻译： 本发明提供了用于降低用于进一步分析的基因组样品的复杂性的新方法，例如直接DNA测序，再测序或SNP呼叫。 该方法使用预选的固定化寡核苷酸探针从含有变性，片段化的基因组核酸的样品中捕获靶核酸分子。 所公开的方法提供来自复杂生物样品的靶核酸的成本效益，灵活和快速富集。
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