公开(公告)号：US20100267071A1

公开(公告)日：2010-10-21

申请号：US12714346

申请日：2010-02-26

申请人： Hashem AKHAVAN-TAFTI ,  Dean G. BINGER ,  Ying Chen ,  Renuka DE SILVA ,  Terri MCLERNON ,  James MENDOZA ,  Bruce H. ODEGAARD ,  Michael SALVATI ,  Nir SHAPIR ,  Wenhua XIE

发明人： Hashem AKHAVAN-TAFTI ,  Dean G. BINGER ,  Ying Chen ,  Renuka DE SILVA ,  Terri MCLERNON ,  James MENDOZA ,  Bruce H. ODEGAARD ,  Michael SALVATI ,  Nir SHAPIR ,  Wenhua XIE

IPC分类号： C12Q1/28 ,  G01N33/53 ,  G01N30/00

CPC分类号： G01N21/76 ,  C09B15/00 ,  C12Q1/28 ,  G01N21/763 ,  G01N33/542 ,  G01N33/582

摘要： Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.

摘要翻译： 公开了用于确定疑似含有分析物的样品中的分析物的方法，试剂，试剂盒和系统，其中所有试剂都可溶于水溶液。 一种测定方法包括在如下条件下处理怀疑含有分析物的样品，使得如果存在分析物，则使活化剂与化学发光化合物反应构型以使其活化。 样品也用试剂处理以减少与分析物无关的信号。 最后，用触发溶液处理样品，从而产生来自活化化学发光化合物的光。 没有试剂与表面或其他固相相关。

公开(公告)号：US09029092B2

公开(公告)日：2015-05-12

申请号：US12714346

申请日：2010-02-26

申请人： Hashem Akhavan-Tafti ,  Dean G. Binger ,  Ying Chen ,  Renuka De Silva ,  Terri McLernon ,  James Mendoza ,  Bruce H. Odegaard ,  Michael Salvati ,  Nir Shapir ,  Wenhua Xie

发明人： Hashem Akhavan-Tafti ,  Dean G. Binger ,  Ying Chen ,  Renuka De Silva ,  Terri McLernon ,  James Mendoza ,  Bruce H. Odegaard ,  Michael Salvati ,  Nir Shapir ,  Wenhua Xie

IPC分类号： G01N33/53 ,  G01N30/00 ,  C12Q1/28 ,  G01N33/542 ,  G01N33/58

CPC分类号： G01N21/76 ,  C09B15/00 ,  C12Q1/28 ,  G01N21/763 ,  G01N33/542 ,  G01N33/582

摘要： Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.

摘要翻译： 公开了用于确定疑似含有分析物的样品中的分析物的方法，试剂，试剂盒和系统，其中所有试剂都可溶于水溶液。 一种测定方法包括在如下条件下处理怀疑含有分析物的样品，使得如果存在分析物，则使活化剂与化学发光化合物反应构型以使其活化。 样品也用试剂处理以减少与分析物无关的信号。 最后，用触发溶液处理样品，从而产生来自活化化学发光化合物的光。 没有试剂与表面或其他固相相关。