公开(公告)号：US20100267071A1

公开(公告)日：2010-10-21

申请号：US12714346

申请日：2010-02-26

申请人： Hashem AKHAVAN-TAFTI ,  Dean G. BINGER ,  Ying Chen ,  Renuka DE SILVA ,  Terri MCLERNON ,  James MENDOZA ,  Bruce H. ODEGAARD ,  Michael SALVATI ,  Nir SHAPIR ,  Wenhua XIE

发明人： Hashem AKHAVAN-TAFTI ,  Dean G. BINGER ,  Ying Chen ,  Renuka DE SILVA ,  Terri MCLERNON ,  James MENDOZA ,  Bruce H. ODEGAARD ,  Michael SALVATI ,  Nir SHAPIR ,  Wenhua XIE

IPC分类号： C12Q1/28 ,  G01N33/53 ,  G01N30/00

CPC分类号： G01N21/76 ,  C09B15/00 ,  C12Q1/28 ,  G01N21/763 ,  G01N33/542 ,  G01N33/582

摘要： Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.

摘要翻译： 公开了用于确定疑似含有分析物的样品中的分析物的方法，试剂，试剂盒和系统，其中所有试剂都可溶于水溶液。 一种测定方法包括在如下条件下处理怀疑含有分析物的样品，使得如果存在分析物，则使活化剂与化学发光化合物反应构型以使其活化。 样品也用试剂处理以减少与分析物无关的信号。 最后，用触发溶液处理样品，从而产生来自活化化学发光化合物的光。 没有试剂与表面或其他固相相关。

公开(公告)号：US20100273189A1

公开(公告)日：2010-10-28

申请号：US12714294

申请日：2010-02-26

申请人： Hashem AKHAVAN-TAFTI ,  Renuka DE SILVA ,  Terri MCLERNON ,  James MENDOZA ,  Michael SALVATI ,  Nir SHAPIR ,  Wenhua XIE

发明人： Hashem AKHAVAN-TAFTI ,  Renuka DE SILVA ,  Terri MCLERNON ,  James MENDOZA ,  Michael SALVATI ,  Nir SHAPIR ,  Wenhua XIE

IPC分类号： G01N33/53

CPC分类号： G01N33/542 ,  C09B15/00 ,  C09B21/00 ,  C09B57/02 ,  C09B67/0033 ,  C12Q1/28 ,  G01N33/582

摘要： Methods, reagents, kits and systems are disclosed for determining an analyte in a sample, the assay method comprising forming a reaction mixture in an aqueous solution, by adding a chemiluminescent-labeled immobilized specific binding member, an activator-labeled specific binding member, a selective signal inhibiting agent, and a sample, wherein the chemiluminescent-labeled immobilized specific binding member and activator-labeled specific binding member bind to analyte present in the sample to form a binding complex, and adding to the reaction mixture a trigger solution to release a detectable chemiluminescent signal correlated to the amount of the analyte-bound binding complex present in the reaction mixture.

摘要翻译： 公开了用于测定样品中分析物的方法，试剂，试剂盒和系统，所述测定方法包括通过添加化学发光标记的固定化特异性结合成员，活化剂标记的特异性结合成员， 选择性信号抑制剂和样品，其中化学发光标记的固定化特异性结合成员和活化剂标记的特异性结合成员结合存在于样品中的分析物以形成结合复合物，并向反应混合物中加入释放 可检测的化学发光信号与反应混合物中存在的分析物结合的结合复合物的量相关。