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    Assay methods using chemiluminescent detection of peroxidase
    1.
    发明授权
    Assay methods using chemiluminescent detection of peroxidase 有权
    使用化学发光检测过氧化物酶的测定方法

    公开(公告)号：US08106203B2

    公开(公告)日：2012-01-31

    申请号：US12476668

    申请日：2009-06-02

    申请人： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Wenhua Xie

    发明人： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Wenhua Xie

    IPC分类号： C07D219/00 ,  C12Q1/28

    CPC分类号： C07D219/02 ,  C07D219/04 ,  C07D219/06 ,  C07D409/04 ,  C07D409/06 ,  C07D411/04 ,  C07D411/06 ,  C07F9/64 ,  C07F9/65586 ,  C12Q1/28 ,  G01N33/581 ,  G01N2333/908

    摘要： Methods for generating chemiluminescence rapidly by reaction of at least one compound comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups with a peroxidase enzyme and a peroxide are disclosed. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs. Use of the methods provides rapid signal generation, achieving a plateau intensity in under one minute. The substrate can be provided in a composition which demonstrates unexpectedly long storage stability.

    摘要翻译： 公开了通过至少一种包含在一个碳上取代的C-C双键的化合物与两个含硫原子的基团与过氧化物酶和过氧化物反应快速产生化学发光的方法。 由此产生的化学发光可用作过氧化物酶或产生过氧化物酶的测定中的可检测信号，并且在使用酶标记的特异性结合对的测定中可以使用。 该方法的使用提供了快速的信号发生，在一分钟内达到了高原强度。 基材可以提供在显示出意想不到的长的储存稳定性的组合物中。

    Nonseparation assay methods
    2.
    发明授权
    Nonseparation assay methods 有权

    公开(公告)号：US08012705B2

    公开(公告)日：2011-09-06

    申请号：US13083195

    申请日：2011-04-08

    申请人： Hashem Akhavan-Tafti

    发明人： Hashem Akhavan-Tafti

    IPC分类号： G01N33/53

    CPC分类号： G01N33/54306

    摘要： Assay methods are disclosed involving specific binding reactions which are simplified compared to known methods. A compound capable of producing chemiluminescence is immobilized on a solid support as is a member of a specific binding pair for capturing an analyte from a sample. An activator compound that activates the chemiluminescent compound and is conjugated to a specific binding pair member is added in excess along with the sample to the solid support. Addition of a trigger solution causes a chemiluminescent reaction at the sites where the activator conjugate has been specifically bound. The assay methods are termed non-separation assays because they do not require removal or separation of excess detection label (activator conjugate) prior to the detection step. The methods are applicable to various types of assays including immunoassays, receptor-ligand assays and nucleic acid hybridization assays.

    Signalling compounds for use in methods of detecting hydrogen peroxide
    4.
    发明授权
    Signalling compounds for use in methods of detecting hydrogen peroxide 有权
    用于检测过氧化氢的方法中的信号化合物

    公开(公告)号：US07704752B2

    公开(公告)日：2010-04-27

    申请号：US12144277

    申请日：2008-06-23

    申请人： Hashem Akhavan-Tafti ,  Robert A. Eickholt ,  Kenneth S. Lauwers ,  Richard S. Handley

    发明人： Hashem Akhavan-Tafti ,  Robert A. Eickholt ,  Kenneth S. Lauwers ,  Richard S. Handley

    IPC分类号： G01N21/76 ,  G01N33/533 ,  C12Q1/28 ,  C07F5/02 ,  C07D321/00

    CPC分类号： C07F5/025 ,  G01N33/533 ,  Y10S436/904 ,  Y10T436/141111 ,  Y10T436/142222 ,  Y10T436/206664

    摘要： Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig-OH or Sig-O− is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.

    摘要翻译： 公开了用于检测过氧化氢源的方法和化合物，其中下式的信号化合物与过氧化物反应。 Sig是非聚合有机基团，B是硼原子，并且每个R独立地选自氢，烷基和芳基，并且可以作为形成环或芳环的直链或支链亚烷基链连接在一起。 通过测量颜色，吸光度，荧光，化学发光或生物发光产生和检测式Sig-OH​​或Sig-O-的可检测产物化合物。 信号化合物本身不具有可检测的性质，或仅仅是非常弱的程度。 该方法可用作过氧化物或产生过氧化物的酶的测定中的可检测信号，以及使用酶标记的特异性结合对的测定中。

    Methods of extracting RNA
    6.
    发明申请
    Methods of extracting RNA 审中-公开
    提取RNA的方法

    公开(公告)号：US20070185322A1

    公开(公告)日：2007-08-09

    申请号：US11703459

    申请日：2007-02-07

    申请人： Hashem Akhavan-Tafti

    发明人： Hashem Akhavan-Tafti

    IPC分类号： C07H21/02

    CPC分类号： C12N15/1006

    摘要： Methods and materials are disclosed for rapid and simple extraction and isolation of RNA from a biological sample involving the use of an acidic solution and a solid phase binding material that has the ability to liberate nucleic acids from biological samples, including whole blood, without first performing any preliminary lysis to disrupt cells or viruses. No detergents or chaotropic substances for lysing cells or viruses are needed or used. Viral, bacterial and mammalian genomic RNA can be isolated using the method of the invention. RNA isolated by the present method is suitable for use in downstream processes such as RT-PCR.

    摘要翻译： 公开了用于从涉及使用酸性溶液和固相结合材料的生物样品中快速和简单地提取和分离RNA的方法和材料，其具有从生物样品（包括全血）中释放核酸的能力，而不首先进行 任何初步裂解破坏细胞或病毒。 不需要或使用用于裂解细胞或病毒的洗涤剂或离液序列物质。 可以使用本发明的方法分离病毒，细菌和哺乳动物基因组RNA。 通过本方法分离的RNA适用于下游过程如RT-PCR。

    Methods of enhancing isolation of RNA from biological samples
    7.
    发明申请
    Methods of enhancing isolation of RNA from biological samples 审中-公开
    增强生物样品中RNA分离的方法

    公开(公告)号：US20060234251A1

    公开(公告)日：2006-10-19

    申请号：US11109366

    申请日：2005-04-19

    申请人： Hashem Akhavan-Tafti

    发明人： Hashem Akhavan-Tafti

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6806 ,  C12Q2527/137 ,  C12Q2527/125

    摘要： A method of enhancing the isolation of ribonucleic acids from samples of biological or cellular material is disclosed which uses a solution of an Agent during binding of nucleic acids onto a solid phase binding material which, after elution, enhances the recovery of RNA. Washing the solid phase and eluting the nucleic acid produces RNA in enhanced yield and/or purity. The use of the new method allows RNA to be captured and released in a form suitable for downstream processing in under five minutes. Preferred Agents according to the invention comprise monomeric, oligomeric, dendrimeric and polymeric organic compounds having multiple positive charges.

    摘要翻译： 公开了一种增强从生物或细胞材料样品中分离核糖核酸的方法，其在核酸结合到固相结合材料上时使用试剂溶液，其在洗脱后增强RNA的回收。 洗涤固相并洗脱核酸以增加的产率和/或纯度产生RNA。 使用新方法可以在五分钟内以适合下游处理的形式捕获和释放RNA。 根据本发明的优选的试剂包含具有多个正电荷的单体，低聚物，树枝状聚合物和聚合物有机化合物。

    Cleavable solid phases for isolating nucleic acids
    8.
    发明申请
    Cleavable solid phases for isolating nucleic acids 审中-公开
    用于分离核酸的可切割固相

    公开(公告)号：US20050106577A1

    公开(公告)日：2005-05-19

    申请号：US10715284

    申请日：2003-11-17

    申请人： Hashem Akhavan-Tafti ,  Renuka Silva ,  Robert Eickholt ,  C. Gundlach ,  Richard Handley ,  Kenneth Lauwers ,  Mark Sandison ,  Wenhua Xie

    发明人： Hashem Akhavan-Tafti ,  Renuka Silva ,  Robert Eickholt ,  C. Gundlach ,  Richard Handley ,  Kenneth Lauwers ,  Mark Sandison ,  Wenhua Xie

    IPC分类号： C07H21/04 ,  C12M1/34 ,  C12Q1/68

    CPC分类号： C07H21/04

    摘要： Solid phase materials for binding nucleic acids and methods of their use are disclosed. The materials feature a cleavable linker portion which can be cleaved to release bound nucleic acids. The solid phase materials comprise a solid support portion comprising a matrix selected from silica, glass, insoluble synthetic polymers, and insoluble polysaccharides to which is attached a nucleic acid binding portion for attracting and binding nucleic acids, the nucleic acid binding portion (NAB) being linked by a cleavable linker portion to the solid support portion. Preferred nucleic acid binding portions comprise a ternary or quaternary onium group. The materials can be in the form of microparticles, fibers, beads, membranes, test tubes or microwells and can further comprise a magnetic core portion. Methods of binding nucleic acids using the cleavable solid supports are disclosed as are their use in methods of isolating or purifying nucleic acids.

    摘要翻译： 公开了用于结合核酸的固相材料及其使用方法。 这些材料具有可切割的接头部分，其可以被切割以释放结合的核酸。 固相材料包括固体支持部分，其包含选自二氧化硅，玻璃，不溶性合成聚合物的基质和与核酸结合部分连接以吸附和结合核酸的不溶性多糖，所述核酸结合部分（NAB）为 通过可切割的连接部分连接到固体支持部分。 优选的核酸结合部分包含三元或四元鎓基团。 材料可以是微粒，纤维，珠，膜，试管或微孔的形式，并且还可以包括磁芯部分。 在分离或纯化核酸的方法中公开了使用可切割固体支持物结合核酸的方法。

    Methods of using cleavable solid phases for isolating nucleic acids
    9.
    发明申请
    Methods of using cleavable solid phases for isolating nucleic acids 审中-公开
    使用可切割固相分离核酸的方法

    公开(公告)号：US20050106576A1

    公开(公告)日：2005-05-19

    申请号：US10714763

    申请日：2003-11-17

    申请人： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Nicole Cilli ,  William Cripps ,  Richard Handley ,  Elizabeth O'Connor ,  Lekkala Reddy ,  Sarada Siripurapu

    发明人： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Nicole Cilli ,  William Cripps ,  Richard Handley ,  Elizabeth O'Connor ,  Lekkala Reddy ,  Sarada Siripurapu

    IPC分类号： C07H21/04 ,  C12Q1/68

    CPC分类号： C07H21/04 ,  C07B2200/11

    摘要： Solid phase materials for binding nucleic acids and methods of their use are disclosed. The materials feature a cleavable linker portion which can be cleaved to release bound nucleic acids. The solid phase materials comprise a solid support portion comprising a matrix selected from silica, glass, insoluble synthetic polymers, and insoluble polysaccharides to which is attached a nucleic acid binding portion for attracting and binding nucleic acids, the nucleic acid binding portion (NAB) being linked by a cleavable linker portion to the solid support portion. Preferred nucleic acid binding portions comprise a ternary or quaternary onium group. The materials can be in the form of microparticles, fibers, beads, membranes, test tubes or microwells and can further comprise a magnetic core portion. Methods of binding nucleic acids using the cleavable solid supports are disclosed as are their use in methods of isolating or purifying nucleic acids.

    摘要翻译： 公开了用于结合核酸的固相材料及其使用方法。 这些材料具有可切割的接头部分，其可以被切割以释放结合的核酸。 固相材料包括固体支持部分，其包含选自二氧化硅，玻璃，不溶性合成聚合物的基质和与核酸结合部分连接以吸附和结合核酸的不溶性多糖，所述核酸结合部分（NAB）为 通过可切割的连接部分连接到固体支持部分。 优选的核酸结合部分包含三元或季铵基团。 材料可以是微粒，纤维，珠，膜，试管或微孔的形式，并且还可以包括磁芯部分。 在分离或纯化核酸的方法中公开了使用可切割固体支持物结合核酸的方法。

    Chemiluminescent dialkyl-substituted 1,2-dioxetane compounds, methods of synthesis and use
    10.
    发明授权
    Chemiluminescent dialkyl-substituted 1,2-dioxetane compounds, methods of synthesis and use 失效
    化学发光二烷基取代的1,2-二氧杂环丁烷化合物，合成和使用方法

    公开(公告)号：US06284899B1

    公开(公告)日：2001-09-04

    申请号：US08999930

    申请日：1997-11-28

    申请人： Arthur Paul Schaap ,  Hashem Akhavan-Tafti

    发明人： Arthur Paul Schaap ,  Hashem Akhavan-Tafti

    IPC分类号： C07D30514

    CPC分类号： G01N33/582 ,  C07C43/1782 ,  C07C43/1787 ,  C07C69/017 ,  C07C2601/14 ,  C07D321/00 ,  C07F7/1804 ,  C07F9/12 ,  C07F9/6551 ,  C07F9/65512 ,  C12Q1/34 ,  G01N33/533

    摘要： A chemiluminescent assay method and compositions are described which use a dialkyl-substituted dioxetane which is deprotected to trigger a chemiluminescent reaction. Chemiluminescent 1,2-dioxetane compounds substituted on the dioxetane ring with two nonspirofused alkyl groups which can be triggered by a reagent to generate light are disclosed. Dialkyl-substituted dioxetanes are useful for the detection of triggering agents including enzymes. The enzyme may be present alone or linked to a member of a specific binding pair in an immunoassay, DNA probe assay or other assay where the enzyme is bound to a reporter molecule.

    摘要翻译： 描述了化学发光测定方法和组合物，其使用被去保护以触发化学发光反应的二烷基取代的二氧杂环丁烷。 公开了在二氧环乙烷环上取代的化学发光的1,2-二氧杂环丁烷化合物，其具有可由试剂触发以产生光的两个非标记烷基。 二烷基取代的二氧杂环丁烷可用于检测包括酶在内的引发剂。 该酶可以在免疫测定，DNA探针测定或其它酶结合报告分子的其它测定中单独存在或连接到特异性结合对的成员。