公开(公告)号：US06402950B1

公开(公告)日：2002-06-11

申请号：US09665860

申请日：2000-09-20

申请人： Paul T. Nix ,  Dennis R. Stocker ,  Michael J. Byers

发明人： Paul T. Nix ,  Dennis R. Stocker ,  Michael J. Byers

IPC分类号： B01D3500

CPC分类号： B01L3/50255 ,  B01L2400/0409 ,  Y10T436/25375 ,  Y10T436/255

摘要： A device which can be used for filtering samples is described. The device is comprised of a plurality of filter wells, each having a wide region and a narrow region. The device is fitted with a filter element and then filled with a gel filtration matrix which is selected so that molecules of a particular molecular size will diffuse into the matrix. The device is centrifuged to remove water from the interstitial regions of the gel filtration matrix, and a sample is then added to the device containing the dried gel filtration matrix. The device is again centrifuged, causing smaller molecules to diffuse into the matrix and allowing larger molecules to exit the device, wherein they are collected and analyzed. The device is capable of filtering sample sizes as low as 1 &mgr;L and can be used in a variety of existing laboratory equipment, including bench-top centrifuges and microcentrifuges. A process for using the device is also disclosed.

摘要翻译： 描述可用于过滤样品的装置。 该装置由多个过滤器孔组成，每个过滤器孔具有宽的区域和窄的区域。 该装置装有过滤元件，然后填充凝胶过滤基质，其被选择使得特定分子尺寸的分子将扩散到基质中。 将装置离心以从凝胶过滤基质的间隙区域除去水，然后将样品加入到含有干燥的凝胶过滤基质的装置中。 该装置再次离心，导致较小的分子扩散到基质中并允许较大的分子离开该装置，其中它们被收集和分析。 该设备能够过滤低至1μL的样品量，可用于各种现有的实验室设备，包括台式离心机和微量离心机。 还公开了一种使用该装置的方法。

公开(公告)号：US4952506A

公开(公告)日：1990-08-28

申请号：US854982

申请日：1986-04-28

申请人： Michael J. Byers ,  Eric G. Isacoff ,  John O. Naples

发明人： Michael J. Byers ,  Eric G. Isacoff ,  John O. Naples

IPC分类号： C12N5/00 ,  C12N1/00 ,  C12N1/16 ,  C12N11/04 ,  C12N11/08

CPC分类号： C12N11/04 ,  Y10S435/942

摘要： Nonanchorage-dependent cells are immobilized for cell culturing or the production of cell products, by contracting an aqueous suspension of the cells with a floc formed by the combination of positively and negatively charged ion exchange resins having particle sizes ranging from about 0.01 to about 1.5 microns. The flocs may be formed either prior to contract with the cells or may be formed in situ in the cell suspension upon separate addition of the resins, preferably by first adding the positively charged resin followed by the negatively charged resin. The resin floc operates as a microcarrier for the cells.

摘要翻译： 固定非依赖性依赖性细胞用于细胞培养或细胞产物的生成，通过用具有约0.01至约1.5微米的粒度范围的带正电荷和带负电荷的离子交换树脂的组合形成的絮凝物收缩细胞的水性悬浮液 。 絮状物可以在与细胞收缩之前形成，或者可以在单独添加树脂时在细胞悬浮液中原位形成，优选首先加入带正电的树脂，然后加入带负电荷的树脂。 树脂絮凝物用作细胞的微载体。