公开(公告)号：US06399337B1

公开(公告)日：2002-06-04

申请号：US09092315

申请日：1998-06-05

申请人： Diane E. Taylor ,  Zhongming Ge

发明人： Diane E. Taylor ,  Zhongming Ge

IPC分类号： C12P1918

CPC分类号： C12N9/1051 ,  C07K2319/00 ,  C12Q1/6886

摘要： A bacterial &agr;1,3-fucosyltransferase gene and deduced amino acid sequence is provided. The gene is useful for preparing &agr;1,3-fucosyltransferase polypeptide, and active fragment thereof, which can be used in the production of oligosaccharides such as Lewis X, Lewis Y, and siayl Lewis X, which are structurally similar to certain tumor-associated carbohydrate antigens found in mammals. These product glycoconjugates also have research and diagnostic utility in the development of assays to detect mammalian tumors. In addition the polypeptide of the invention can be used to develop diagnostic and research assays to determine the presence of H. pylori in human specimens.

摘要翻译： 提供细菌α1,3-岩藻糖基转移酶基因和推导的氨基酸序列。 该基因可用于制备α1,3-岩藻糖基转移酶多肽及其活性片段，其可用于生产低聚糖例如Lewis X，Lewis Y和siayl Lewis X，其结构上类似于某些肿瘤相关碳水化合物 在哺乳动物中发现的抗原。 这些产品糖缀合物在开发检测哺乳动物肿瘤的检测中也具有研究和诊断效用。 此外，本发明的多肽可用于开发诊断和研究测定以确定幽门螺杆菌在人标本中的存在。

公开(公告)号：US07029891B2

公开(公告)日：2006-04-18

申请号：US10392098

申请日：2003-03-17

申请人： Diane E. Taylor ,  Zhongming Ge

发明人： Diane E. Taylor ,  Zhongming Ge

IPC分类号： C12N9/10 ,  C12N15/54

CPC分类号： C12N9/1051 ,  C07K2319/00 ,  C12Q1/6886

摘要： A bacterial α1,3-fucosyltransferase gene and deduced amino acid sequence is provided. The gene is useful for preparing α1,3-fucosyltransferase polypeptide, and active fragment thereof, which can be used in the production of oligosaccharides such as Lewis X, Lewis Y, and siayl Lewis X, which are structurally similar to certain tumor-associated carbohydrate antigens found in mammals. These product glycoconjugates also have research and diagnostic utility in the development of assays to detect mammalian tumors. In addition the polypeptide of the invention can be used to develop diagnostic and research assays to determine the presence of H. pylori in human specimens.

公开(公告)号：US06962806B2

公开(公告)日：2005-11-08

申请号：US10189977

申请日：2002-07-03

申请人： Diane E. Taylor ,  Zhongming Ge

发明人： Diane E. Taylor ,  Zhongming Ge

IPC分类号： C12N1/21 ,  C12N9/10 ,  C12N15/54 ,  C12Q1/68

CPC分类号： C12N9/1051 ,  C07K2319/00 ,  C12Q1/6886

摘要： A bacterial α1,3-fucosyltransferase gene and deduced amino acid sequence is provided. The gene is useful for preparing α1,3-fucosyltransferase polypeptide, and active fragment thereof, which can be used in the production of oligosaccharides such as Lewis X, Lewis Y, and siayl Lewis X, which are structurally similar to certain tumor-associated carbohydrate antigens found in mammals. These product glycoconjugates also have research and diagnostic utility in the development of assays to detect mammalian tumors. In addition the polypeptide of the invention can be used to develop diagnostic and research assays to determine the presence of H. pylori in human specimens.

摘要翻译： 提供细菌α1,3-岩藻糖基转移酶基因和推导的氨基酸序列。 该基因可用于制备α1,3-岩藻糖基转移酶多肽及其活性片段，其可用于生产低聚糖例如Lewis X，Lewis Y和siayl Lewis X，其结构上类似于某些肿瘤相关碳水化合物 在哺乳动物中发现的抗原。 这些产品糖缀合物在开发检测哺乳动物肿瘤的检测中也具有研究和诊断效用。 此外，本发明的多肽可用于开发诊断和研究测定以确定幽门螺杆菌在人标本中的存在。

公开(公告)号：US07524655B2

公开(公告)日：2009-04-28

申请号：US10521138

申请日：2005-12-06

申请人： Karl F. Johnson ,  Daniel James Bezila ,  Diane E. Taylor ,  Joanne Simala-Grant ,  David Rasko

发明人： Karl F. Johnson ,  Daniel James Bezila ,  Diane E. Taylor ,  Joanne Simala-Grant ,  David Rasko

IPC分类号： C12P19/18 ,  C12N9/10 ,  C12N9/52

CPC分类号： C12P21/005 ,  C12N9/1051

摘要： This invention provides nucleic acid and amino acid sequences of fucosyltransferases from Helicobactor pylori. The invention also provides methods to use the fucosyltransferases to synthesize oligosaccharides, glycoproteins, and glycolipids.

摘要翻译： 本发明提供了幽门螺旋杆菌的岩藻糖基转移酶的核酸和氨基酸序列。 本发明还提供了使用岩藻糖基转移酶合成低聚糖，糖蛋白和糖脂的方法。

公开(公告)号：US06534298B2

公开(公告)日：2003-03-18

申请号：US09733524

申请日：2000-12-07

申请人： Diane E. Taylor ,  Zhongming Ge

发明人： Diane E. Taylor ,  Zhongming Ge

IPC分类号： C12N910

CPC分类号： C12N9/1051 ,  C07K2319/00 ,  C12Q1/6886

摘要： A bacterial &agr;1,3-fucosyltransferase gene and deduced amino acid sequence is provided. The gene is useful for preparing &agr;1,3-fucosyltransferase polypeptide, and active fragment thereof, which can be used in the production of oligosaccharides such as Lewis X, Lewis Y, and siayl Lewis X, which are structurally similar to certain tumor-associated carbohydrate antigens found in mammals. These product glycoconjugates also have research and diagnostic utility in the development of assays to detect mammalian tumors. In addition the polypeptide of the invention can be used to develop diagnostic and research assays to determine the presence of H. pylori in human specimens.

公开(公告)号：US07166449B2

公开(公告)日：2007-01-23

申请号：US10120319

申请日：2002-04-09

申请人： Diane E. Taylor ,  Zhongming Ge

发明人： Diane E. Taylor ,  Zhongming Ge

IPC分类号： C12P19/18 ,  C12N9/10 ,  C12N15/54

CPC分类号： C12N9/1051 ,  C07K2319/00 ,  C12Q1/6886

摘要： A bacterial α1,3-fucosyltransferase gene and deduced amino acid sequence is provided. The gene is useful for preparing α1,3-fucosyltransferase polypeptide, and active fragment thereof, which can be used in the production of oligosaccharides such as Lewis X, Lewis Y, and siayl Lewis X, which are structurally similar to certain tumor-associated carbohy-drate antigens found in mammals. These product glycoconjugates also have research and diagnostic utility in the development of assays to detect mammalian tumors. In addition the polypeptide of the invention can be used to develop diagnostic and research assays to determine the presence of H. pylori in human specimens.

摘要翻译： 提供细菌α1,3-岩藻糖基转移酶基因和推导的氨基酸序列。 该基因可用于制备α1,3-岩藻糖基转移酶多肽及其活性片段，其可用于生产低聚糖例如Lewis X，Lewis Y和siayl Lewis X，其结构上类似于某些肿瘤相关性碳水化合物 在哺乳动物中发现的抗原。 这些产品糖缀合物在开发检测哺乳动物肿瘤的检测中也具有研究和诊断效用。 此外，本发明的多肽可用于开发诊断和研究测定以确定幽门螺杆菌在人标本中的存在。