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1.发明授权Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same 有权表现出在细胞内环境中增强的稳定性的免疫球蛋白框架及鉴定其的方法公开(公告)号：US08853362B2
公开(公告)日：2014-10-07
申请号：US10515241
申请日：2003-05-21
IPC分类号： C07K16/00
CPC分类号： C07K16/00 , A61K48/00 , C07K2317/21 , C07K2317/55 , C07K2317/567 , C07K2317/622 , C07K2317/80 , C07K2317/82 , C07K2317/94 , G01N33/6857
摘要： Compositions are provided, which can be used as frameworks for the creation of very stable and soluble single-chain Fv antibody fragments. These frameworks have been selected for intracellular performance and are thus ideally suited for the creation of scFv antibody fragments or scFv antibody libraries for applications where stability and solubility are limiting factors for the performance of antibody fragments, such as in the reducing environment of a cell. Such frameworks can also be used to identify highly conserved residues and consensus sequences which demonstrate enhanced solubility and stability.
摘要翻译： 提供组合物，其可用作产生非常稳定和可溶性单链Fv抗体片段的框架。 这些框架已经被选择用于细胞内性能，因此理想地适用于产生scFv抗体片段或scFv抗体文库，用于其中稳定性和溶解度是抗体片段表现的限制因素，例如在细胞的还原环境中。 此类框架还可用于鉴定高度保守的残基和共有序列，这些序列表现出增强的溶解度和稳定性。
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2.发明授权Intrabodies with defined framework that is stable in a reducing environment and applications thereof 有权具有在还原环境中稳定的具有框架的体内抗体及其应用公开(公告)号：US07258986B2
公开(公告)日：2007-08-21
申请号：US10169179
申请日：2000-12-18
CPC分类号： C07K16/005 , A61K2039/505 , C07K16/00 , C07K16/14 , C07K2317/622 , C07K2317/80 , C07K2318/10 , C07K2319/00 , C12N15/1055 , C12N15/1093
摘要： A method for the isolation of CDRs in a defined framework that is stable and soluble in reducing environment is described as well as thus obtainable scFv. Starting from such scFv with defined framework a scFv library can be generated wherein the framework is conserved while at least one complementary determining region (CDR) is randomized. Such library, e.g. in yeast cells, is suitable for screening for antibody/CDR-interactions or for screening for antibodies.
摘要翻译： 描述了在限定的框架中分离CDR的方法，其稳定和可溶于还原环境，以及由此获得的scFv。 从具有定义框架的这种scFv开始，可以生成scFv文库，其中框架是保守的，而至少一个互补决定区（CDR）是随机的。 这样的文库，例如 在酵母细胞中，适用于筛选抗体/ CDR相互作用或筛选抗体。
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公开(公告)号：US08399624B1
公开(公告)日：2013-03-19
申请号：US12823488
申请日：2010-06-25
申请人： Dominik Escher
发明人： Dominik Escher
IPC分类号： C12P21/08
CPC分类号： C07K16/464 , C07H21/00 , C07K16/00 , C07K16/18 , C07K16/241 , C07K2317/14 , C07K2317/24 , C07K2317/55 , C07K2317/565 , C07K2317/567 , C07K2317/622 , C07K2317/92 , C07K2317/94
摘要： The present invention relates to an antibody acceptor framework and to methods for grafting non-human antibodies, e.g., rabbit antibodies, using a particularly well suited antibody acceptor framework. Antibodies generated by the methods of the invention are useful in a variety of diagnostic and therapeutic applications.
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4.发明申请Intrabodies with defined framework that is stable in a reducing environment and applications thereof 审中-公开具有在还原环境中稳定的具有框架的体内抗体及其应用公开(公告)号：US20080233110A1
公开(公告)日：2008-09-25
申请号：US11894097
申请日：2007-08-20
IPC分类号： A61K39/395 , C07K16/00 , C40B30/06 , C40B50/00 , C40B40/10
CPC分类号： C07K16/005 , A61K2039/505 , C07K16/00 , C07K16/14 , C07K2317/622 , C07K2317/80 , C07K2318/10 , C07K2319/00 , C12N15/1055 , C12N15/1093
摘要： A method for the isolation of CDRs in a defined framework that is stable and soluble in reducing environment is described as well as thus obtainable scFv. Starting from such scFv with defined framework a scFv library can be generated wherein the framework is conserved while at least one complementary determining region (CDR) is randomized. Such library, e.g. in yeast cells, is suitable for screening for antibody/CDR-interactions or for screening for antibodies.
摘要翻译： 描述了在限定的框架中分离CDR的方法，其稳定和可溶于还原环境，以及由此获得的scFv。 从具有定义框架的这种scFv开始，可以生成scFv文库，其中框架是保守的，而至少一个互补决定区（CDR）是随机的。 这样的文库，例如 在酵母细胞中，适用于筛选抗体/ CDR相互作用或筛选抗体。
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公开(公告)号：US06821728B1
公开(公告)日：2004-11-23
申请号：US09720106
申请日：2001-03-09
申请人： Dominik Escher , Alcide Barberis
发明人： Dominik Escher , Alcide Barberis
IPC分类号： C12Q168
CPC分类号： C07K14/52 , C07K14/4746 , C07K2319/02 , C12N15/1055 , C12N15/81 , C12Q1/6897
摘要： The present invention concerns a screening system for screening effector peptides that can act as agonists or antagonists, as well as means to produce such a screening system, in particular a specific DNA library encoding peptides. In order to perform the screening, host cells comprising a selection system are transformed with the DNA library and the thus obtained screening system is then cultivated under conditions selectively allowing survival of cells with desired interaction.
摘要翻译： 本发明涉及用于筛选可用作激动剂或拮抗剂的效应肽的筛选系统，以及产生这种筛选系统，特别是编码肽的特异性DNA文库的方法。 为了进行筛选，用DNA文库转化包含选择系统的宿主细胞，然后在选择性允许具有期望相互作用的细胞存活的条件下培养如此获得的筛选系统。
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公开(公告)号：US08399625B1
公开(公告)日：2013-03-19
申请号：US12823551
申请日：2010-06-25
申请人： Dominik Escher
发明人： Dominik Escher
IPC分类号： C12P21/08
CPC分类号： C07K16/464 , C07H21/00 , C07K16/00 , C07K16/18 , C07K16/241 , C07K2317/14 , C07K2317/24 , C07K2317/55 , C07K2317/565 , C07K2317/567 , C07K2317/622 , C07K2317/92 , C07K2317/94
摘要： The present invention relates to an antibody acceptor framework and to methods for grafting non-human antibodies, e.g., rabbit antibodies, using a particularly well suited antibody acceptor framework. Antibodies generated by the methods of the invention are useful in a variety of diagnostic and therapeutic applications.
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7.发明授权公开(公告)号：US07258985B2
公开(公告)日：2007-08-21
申请号：US09750424
申请日：2000-12-28
CPC分类号： C07K16/005 , A61K2039/505 , C07K16/00 , C07K16/14 , C07K2317/622 , C07K2317/80 , C07K2318/10 , C07K2319/00 , C12N15/1055 , C12N15/1093
摘要： A method for the isolation of CDRs in a defined framework that is stable and soluble in reducing environment is described as well as thus obtainable scFv. Starting from such scFv with defined framework a scFv library can be generated wherein the framework is conserved while at least one complementary determining region (CDR) is randomized. Such library, e.g. in yeast cells, is suitable for screening for antibody/CDR-interactions or for screening for antibodies.
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