公开(公告)号：US20120005788A1

公开(公告)日：2012-01-05

申请号：US13256559

申请日：2010-03-17

申请人： Peter Richard ,  Marilyn Wiebe ,  Mervi Toivari ,  Dominik Mojzita ,  Laura Ruohonen ,  Merja Penttilä

发明人： Peter Richard ,  Marilyn Wiebe ,  Mervi Toivari ,  Dominik Mojzita ,  Laura Ruohonen ,  Merja Penttilä

IPC分类号： A01H15/00 ,  C12N1/15 ,  C04B18/18 ,  C12P7/40

CPC分类号： C12P7/58 ,  C12N9/0006

摘要： Provided is a method for producing xylonic acid from xylose with a recombinant fungal strain that is genetically modified to express a xylose dehydrogenase gene, which is able to convert xylose to xylonolactone, which is spontaneously or enzymatically hydrolysed to xylonic acid. The xylonic acid is excreted outside the host cell. Xylonate production may be coupled with xylitol production. Alternatively, if xylitol production is not desired, its production is reduced by removing the aldose reductase (or specific xylose reductase) enzyme, which converts xylose to xylitol. Expression of a heterologous lactonase encoding gene may result in higher acid concentrations. The method is suitable for producing xylonic acid from a hemicellulose hydrolysate such as hydrolysed lignocellulosic plant biomass.

摘要翻译： 本发明提供了一种从木糖中生产木糖的方法，该重组真菌菌株被遗传修饰以表达木糖脱氢酶基因，其能够将木糖转化为己内酰胺，该木糖自发或酶促水解成木糖酸。 木糖酸在宿主细胞外排出。 木糖苷生产可能与木糖醇生产相结合。 或者，如果不需要木糖醇生产，则通过除去将木糖转化为木糖醇的醛糖还原酶（或特异性木糖还原酶）酶来降低其产生。 异源内含子编码基因的表达可能导致较高的酸浓度。 该方法适用于从半纤维素水解产物如水解木质纤维素植物生物质中生产木糖酸。

公开(公告)号：US09315834B2

公开(公告)日：2016-04-19

申请号：US13256559

申请日：2010-03-17

申请人： Peter Richard ,  Marilyn Wiebe ,  Mervi Toivari ,  Dominik Mojzita ,  Laura Ruohonen ,  Merja Penttilä

发明人： Peter Richard ,  Marilyn Wiebe ,  Mervi Toivari ,  Dominik Mojzita ,  Laura Ruohonen ,  Merja Penttilä

IPC分类号： C12P7/58 ,  C12N9/06 ,  C08B31/00 ,  C12N9/04

CPC分类号： C12P7/58 ,  C12N9/0006

摘要： Provided is a method for producing xylonic acid from xylose with a recombinant fungal strain that is genetically modified to express a xylose dehydrogenase gene, which is able to convert xylose to xylonolactone, which is spontaneously or enzymatically hydrolyzed to xylonic acid. The xylonic acid is excreted outside the host cell. Xylonate production may be coupled with xylitol production. Alternatively, if xylitol production is not desired, its production is reduced by removing the aldose reductase (or specific xylose reductase) enzyme, which converts xylose to xylitol. Expression of a heterologous lactonase encoding gene may result in higher acid concentrations. The method is suitable for producing xylonic acid from a hemicellulose hydrolysate such as hydrolyzed lignocellulosic plant biomass.

摘要翻译： 本发明提供了一种从木糖中生产木糖的方法，该重组真菌菌株被遗传修饰以表达木糖脱氢酶基因，其能够将木糖转化为己内酰胺，该木糖自发或酶促水解成木糖酸。 木糖酸在宿主细胞外排出。 木糖苷生产可能与木糖醇生产相结合。 或者，如果不需要木糖醇生产，则通过除去将木糖转化为木糖醇的醛糖还原酶（或特异性木糖还原酶）酶来降低其产生。 异源内含子编码基因的表达可能导致较高的酸浓度。 该方法适用于从半纤维素水解产物如水解木质纤维素植物生物质生产木糖酸。