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    Manufacture of xylonic acid
    1.
    发明授权
    Manufacture of xylonic acid 有权
    木糖酸的制造

    公开(公告)号：US09315834B2

    公开(公告)日：2016-04-19

    申请号：US13256559

    申请日：2010-03-17

    申请人： Peter Richard ,  Marilyn Wiebe ,  Mervi Toivari ,  Dominik Mojzita ,  Laura Ruohonen ,  Merja Penttilä

    发明人： Peter Richard ,  Marilyn Wiebe ,  Mervi Toivari ,  Dominik Mojzita ,  Laura Ruohonen ,  Merja Penttilä

    IPC分类号： C12P7/58 ,  C12N9/06 ,  C08B31/00 ,  C12N9/04

    CPC分类号： C12P7/58 ,  C12N9/0006

    摘要： Provided is a method for producing xylonic acid from xylose with a recombinant fungal strain that is genetically modified to express a xylose dehydrogenase gene, which is able to convert xylose to xylonolactone, which is spontaneously or enzymatically hydrolyzed to xylonic acid. The xylonic acid is excreted outside the host cell. Xylonate production may be coupled with xylitol production. Alternatively, if xylitol production is not desired, its production is reduced by removing the aldose reductase (or specific xylose reductase) enzyme, which converts xylose to xylitol. Expression of a heterologous lactonase encoding gene may result in higher acid concentrations. The method is suitable for producing xylonic acid from a hemicellulose hydrolysate such as hydrolyzed lignocellulosic plant biomass.

    摘要翻译： 本发明提供了一种从木糖中生产木糖的方法，该重组真菌菌株被遗传修饰以表达木糖脱氢酶基因，其能够将木糖转化为己内酰胺，该木糖自发或酶促水解成木糖酸。 木糖酸在宿主细胞外排出。 木糖苷生产可能与木糖醇生产相结合。 或者，如果不需要木糖醇生产，则通过除去将木糖转化为木糖醇的醛糖还原酶（或特异性木糖还原酶）酶来降低其产生。 异源内含子编码基因的表达可能导致较高的酸浓度。 该方法适用于从半纤维素水解产物如水解木质纤维素植物生物质生产木糖酸。

    MANUFACTURE OF XYLONIC ACID
    2.
    发明申请
    MANUFACTURE OF XYLONIC ACID 有权
    制备方法

    公开(公告)号：US20120005788A1

    公开(公告)日：2012-01-05

    申请号：US13256559

    申请日：2010-03-17

    申请人： Peter Richard ,  Marilyn Wiebe ,  Mervi Toivari ,  Dominik Mojzita ,  Laura Ruohonen ,  Merja Penttilä

    发明人： Peter Richard ,  Marilyn Wiebe ,  Mervi Toivari ,  Dominik Mojzita ,  Laura Ruohonen ,  Merja Penttilä

    IPC分类号： A01H15/00 ,  C12N1/15 ,  C04B18/18 ,  C12P7/40

    CPC分类号： C12P7/58 ,  C12N9/0006

    摘要： Provided is a method for producing xylonic acid from xylose with a recombinant fungal strain that is genetically modified to express a xylose dehydrogenase gene, which is able to convert xylose to xylonolactone, which is spontaneously or enzymatically hydrolysed to xylonic acid. The xylonic acid is excreted outside the host cell. Xylonate production may be coupled with xylitol production. Alternatively, if xylitol production is not desired, its production is reduced by removing the aldose reductase (or specific xylose reductase) enzyme, which converts xylose to xylitol. Expression of a heterologous lactonase encoding gene may result in higher acid concentrations. The method is suitable for producing xylonic acid from a hemicellulose hydrolysate such as hydrolysed lignocellulosic plant biomass.

    摘要翻译： 本发明提供了一种从木糖中生产木糖的方法，该重组真菌菌株被遗传修饰以表达木糖脱氢酶基因，其能够将木糖转化为己内酰胺，该木糖自发或酶促水解成木糖酸。 木糖酸在宿主细胞外排出。 木糖苷生产可能与木糖醇生产相结合。 或者，如果不需要木糖醇生产，则通过除去将木糖转化为木糖醇的醛糖还原酶（或特异性木糖还原酶）酶来降低其产生。 异源内含子编码基因的表达可能导致较高的酸浓度。 该方法适用于从半纤维素水解产物如水解木质纤维素植物生物质中生产木糖酸。

    Engineering fungi for the utilisation of L-arabinose
    6.
    发明授权
    Engineering fungi for the utilisation of L-arabinose 失效
    工程真菌利用L-阿拉伯糖

    公开(公告)号：US07527951B2

    公开(公告)日：2009-05-05

    申请号：US10257821

    申请日：2002-02-15

    申请人： John Londesborough ,  Merja Penttilä ,  Peter Richard

    发明人： John Londesborough ,  Merja Penttilä ,  Peter Richard

    IPC分类号： C12P7/06 ,  C12N1/18 ,  C12N1/16 ,  C12N15/74

    CPC分类号： C12N9/0006 ,  C12Y101/0101 ,  C12Y101/01012 ,  Y02E50/17

    摘要： A fungal microorganism can be engineered by means of genetic engineering to utilise L-arabinose. The genes of the L-arabinose pathway, which were unknown, i.e. L-arabinitol 4-dehydrogenase and L-xylulose reductase, were identified. These genes, together with the known genes of the L-arabinose pathway, form a functional pathway. This pathway can be introduced to a fungus, which is completely or partially lacking this pathway.

    摘要翻译： 真菌微生物可以通过遗传工程进行工程化以利用L-阿拉伯糖。 鉴定了未知的L-阿拉伯糖途径的基因，即L-阿拉伯糖醇4-脱氢酶和L-木酮糖还原酶。 这些基因与已知的L-阿拉伯糖途径的基因一起形成功能途径。 该途径可以被引入真菌，其完全或部分缺乏该途径。

    Fungal micro-organism having an increased ability to carry out biotechnological process(es)
    7.
    发明申请
    Fungal micro-organism having an increased ability to carry out biotechnological process(es) 审中-公开
    具有提高生物技术过程能力的真菌微生物

    公开(公告)号：US20050106734A1

    公开(公告)日：2005-05-19

    申请号：US10494030

    申请日：2002-10-29

    申请人： Peter Richard ,  John Londesborough ,  Merja Penttila ,  Ritva Verho

    发明人： Peter Richard ,  John Londesborough ,  Merja Penttila ,  Ritva Verho

    IPC分类号： C12N15/09 ,  C12N1/14 ,  C12N1/19 ,  C12N9/02 ,  C12P7/06 ,  C12P7/62 ,  C12N1/18 ,  C12N15/74

    CPC分类号： C12N9/0008 ,  Y02E50/17

    摘要： The present invention relates to fungal microorganism having an increased ability to carry out biotechnological process(es). In particular, the invention relates to improving the regeneration of redox cofactors in biotechnological processes where useful products are produced from biomass containing pentoses. According to the invention, the microorganism is transformed with a DNA sequence encoding an NADP linked glyceraldehyde 3-phosphate dehydrogenase. The invention can be used to provide useful products for mankind from biological materials, including e.g. agricultural and forestry products, municipal waste. Examples of such useful products are ethanol, lactic acid, polyhydroxyalkanoates, amino acids, fats, vitamins, nucleotides and a wide variety of enzymes and pharmaceuticals.

    摘要翻译： 本发明涉及具有提高生物技术过程能力的真菌微生物。 特别地，本发明涉及在生物技术过程中改进氧化还原辅因子的再生，其中有用产物由含有戊糖的生物质产生。 根据本发明，用编码NADP连接的甘油醛-3-磷酸脱氢酶的DNA序列转化微生物。 本发明可用于从生物材料为人类提供有用的产品，包括例如， 农林产品，城市垃圾。 这些有用产品的实例是乙醇，乳酸，聚羟基链烷酸酯，氨基酸，脂肪，维生素，核苷酸和多种酶和药物。

    CONVERSION OF HEXURONIC ACID TO HEXARIC ACID
    8.
    发明申请
    CONVERSION OF HEXURONIC ACID TO HEXARIC ACID 有权
    十六烷酸转化为十六烷酸

    公开(公告)号：US20120045804A1

    公开(公告)日：2012-02-23

    申请号：US13141736

    申请日：2009-12-22

    申请人： Harry Boer ,  Satu Hildich ,  Peter Richard ,  Merja Penttila

    发明人： Harry Boer ,  Satu Hildich ,  Peter Richard ,  Merja Penttila

    IPC分类号： C12P7/44 ,  C12N1/15

    CPC分类号： C12P7/44 ,  C12N9/0006 ,  C12P7/58

    摘要： The present invention relates to a method and microbial host strain for converting a hexuronic acid to a hexaric acid. In particular, the invention relates to the con-version of D-galacturonic acid to meso-galactaric acid (mucic acid). The invention also concerns an isolated nucleotide sequence. According to the present method a microbial host strain genetically modified to express uronate dehydrogenase enzyme (EC 1.1.1.203) is contacted with a biomaterial comprising hexuronic acid and the con-version products are recovered. By using the recombinant microorganisms of the present invention it is possible to treat biomaterials comprising hexuronic acids and thereby decrease the amount of hexuronic acids released to the environment.

    摘要翻译： 本发明涉及一种将己糖醛酸转化为六酸的方法和微生物宿主菌株。 特别地，本发明涉及D-半乳糖醛酸与内消旋半乳糖酸（粘酸）的对接。 本发明还涉及分离的核苷酸序列。 根据本方法，将遗传修饰以表达糖醛酸脱氢酶（EC 1.1.1.203）的微生物宿主菌与包含己糖醛酸的生物材料接触，并回收相关产物。 通过使用本发明的重组微生物，可以处理包含己糖醛酸的生物材料，从而减少释放到环境中的己糖醛酸的量。

    Engineering Fungi for the Utilisation of L-Arabinose
    9.
    发明申请
    Engineering Fungi for the Utilisation of L-Arabinose 审中-公开
    工程真菌利用L-阿拉伯糖

    公开(公告)号：US20090209016A1

    公开(公告)日：2009-08-20

    申请号：US12368509

    申请日：2009-02-10

    申请人： John Londesborough ,  Merja Penttila ,  Peter Richard

    发明人： John Londesborough ,  Merja Penttila ,  Peter Richard

    IPC分类号： C12P7/56 ,  C12P7/06 ,  C12P7/18 ,  C12P1/02

    CPC分类号： C12N9/0006 ,  C12Y101/0101 ,  C12Y101/01012 ,  Y02E50/17

    摘要： A fungal microorganism can be engineered by means of genetic engineering to utilise L-arabinose. The genes of the L-arabinose pathway, which were unknown, i.e. L-arabinitol 4-dehydrogenase and L-xylulose reductase, were identified. These genes, together with the known genes of the L-arabinose pathway, form a functional pathway. This pathway can be introduced to a fungus, which is completely or partially lacking this pathway.

    摘要翻译： 真菌微生物可以通过遗传工程进行工程化以利用L-阿拉伯糖。 鉴定了未知的L-阿拉伯糖途径的基因，即L-阿拉伯糖醇4-脱氢酶和L-木酮糖还原酶。 这些基因与已知的L-阿拉伯糖途径的基因一起形成功能途径。 该途径可以被引入真菌，其完全或部分缺乏该途径。

    Enzyme for an in Vivo and in Vitro Utilisation of Carbohydrates
    10.
    发明申请
    Enzyme for an in Vivo and in Vitro Utilisation of Carbohydrates 审中-公开
    体内和体外利用碳水化合物的酶

    公开(公告)号：US20070259407A1

    公开(公告)日：2007-11-08

    申请号：US10571597

    申请日：2004-09-13

    申请人： Ritva Verho ,  Peter Richard ,  Merja Pentillae

    发明人： Ritva Verho ,  Peter Richard ,  Merja Pentillae

    IPC分类号： C12P19/00 ,  C07H21/04 ,  C12N1/00 ,  C12N1/21 ,  C12N15/00 ,  C12N9/00 ,  C12P1/00

    CPC分类号： C12N9/0006

    摘要： The invention is directed to an isolated DNA molecule which includes a gene encoding an enzyme protein which has an NADH dependent L-xylulose reductase activity. The DNA sequence encoding the enzyme protein was identified. The invention is further directed to a microorganism transformed with said DNA molecule of the invention, as well as to the NADH dependent L-xylulose reductase. The invention can be utilised for the conversion of biomaterial, e.g. industrial waste material, containing carbohydrates to useful end products

    摘要翻译： 本发明涉及一种分离的DNA分子，其包括编码具有NADH依赖性L-木酮糖还原酶活性的酶蛋白的基因。 鉴定出编码酶蛋白的DNA序列。 本发明还涉及用本发明的所述DNA分子以及NADH依赖性L-木酮糖还原酶转化的微生物。 本发明可用于生物材料的转化，例如， 工业废料，含有碳水化合物用于有用的最终产品